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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2002;22:329.)
© 2002 American Heart Association, Inc.

Thrombosis

Platelet Shape Changes and Adhesion Under High Shear Flow

Mitsuhiro Kuwahara; Mitsuhiko Sugimoto; Shizuko Tsuji; Hideto Matsui; Tomohiro Mizuno; Shigeki Miyata; Akira Yoshioka

From the Department of Pediatrics, Nara Medical University, Kashihara, Nara, Japan.

Reprint requests to Mitsuhiko Sugimoto, MD, Department of Pediatrics, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8522, Japan. E-mail sugi-ped{at}naramed-u.ac.jp

Recent studies have revealed that the platelet adhesive process under flow is tightly regulated by multiple ligand-receptor interactions. However, platelet morphological changes during this process, particularly its physiological relevance, remain unknown under blood flow conditions. Using epifluorescence and scanning electron microscopy, we evaluated the real-time changes in platelet morphology during a platelet adhesive process on a von Willebrand factor-coated surface under physiological high shear flow in a perfusion chamber. Here, we show that dynamic platelet shape changes occurring during distinct phases of the adhesive process are precisely regulated by "inside-out" and "outside-in" integrin signals and are also a key regulatory element in successful platelet thrombogenesis opposing rapid blood flow in vivo.

Key Words: platelet adhesion • von Willebrand factor • glycoprotein Ib • shape changes • high shear rate

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