doi: 10.1161/hq0801.093653
© 2001 American Heart Association, Inc.
Vascular Biology |
Involvement of Rho-Kinase and the Actin Filament Network in Angiotensin II–Induced Contraction and Extracellular Signal–Regulated Kinase Activity in Intact Rat Mesenteric Resistance Arteries
From Institut National de la Santé et de la Recherche Médicale (INSERM) U 541 (K.M., L.L., D.G., A.T., D.H.), IFR 6, Université Paris VII, Paris, France; the Department of Physiology (B.I.L.), AP-HP-Hôpital Lariboisiére, Paris, France; and the Department of Pharmacology (L.B.T.), University of Aarhus, Aarhus, Denmark.
Correspondence to K. Matrougui, PhD, INSERM U 141, Hôpital Lariboisiére, 41 Bd de la Chapelle, 75475 Paris, cedex 10, France. E-mail matrougui9{at}hotmail.com
Abstract—— We have previously shown that angiotensin II (Ang II) and pressure increase extracellular signal-regulated kinase (ERK) 1/2 activity synergistically in intact, pressurized resistance arteries in vitro. However, the mechanisms by which pressure and Ang II activate ERK1/2 in intact resistance arteries remain to be determined. The purpose of the present study was to investigate the involvement of Rho-kinase and the actin filament network in Ang II- and pressure-induced ERK1/2 activation, as well as in the contractile response induced by Ang II. Mesenteric resistance arteries (200 to 300 µm) were isolated, mounted in an arteriograph, and stimulated by pressure, Ang II, or both. Activation of ERK1/2 was then measured by an in-gel assay. In mesenteric resistance arteries maintained at 70 mm Hg, Ang II (0.1 µmol/L) induced contraction (29±1.4% of phenylephrine, 10 µmol/L-induced contraction) and significantly increased ERK1/2 activity. Selective inhibition of Rho-kinase by Y-27632 (10 µmol/L) and selective disruption of the actin filament network by cytochalasin B (10 µmol/L) both decreased the Ang II-induced contraction by 78±1.2% and 87±1.9%, respectively, and significantly diminished ERK1/2 activity. In the absence of Ang II, increasing intraluminal pressure from 0 to 70 or 120 mm Hg increased ERK1/2 activity. ERK1/2 activity at 120 mm Hg was similar to that observed at 70 mm Hg in the presence of Ang II. Pressure-induced ERK1/2 activation was markedly attenuated by cytochalasin B but not by Y-27632. These results indicate that whereas pressure-induced ERK1/2 activation requires an intact actin filament network, but not Rho-kinase, the activation of ERK1/2 and the contraction induced by Ang II require both Rho-kinase and an intact actin filament network in isolated, intact mesenteric resistance arteries.
Key Words: Ang II • MAP-kinase • Rho-kinase • actin filament network • resistance arteries
This article has been cited by other articles:
 |
|
 |
|
  Z. Ying, F. R.C. Giachini, R. C. Tostes, and R. C. Webb PYK2/PDZ-RhoGEF Links Ca2+ Signaling to RhoA Arterioscler Thromb Vasc Biol, October 1, 2009; 29(10): 1657 - 1663. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Belmadani, M. Zerfaoui, H. A. Boulares, D. I. Palen, and K. Matrougui Microvessel vascular smooth muscle cells contribute to collagen type I deposition through ERK1/2 MAP kinase, {alpha}v{beta}3-integrin, and TGF-{beta}1 in response to ANG II and high glucose Am J Physiol Heart Circ Physiol, July 1, 2008; 295(1): H69 - H76. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Albinsson and P. Hellstrand Integration of signal pathways for stretch-dependent growth and differentiation in vascular smooth muscle Am J Physiol Cell Physiol, August 1, 2007; 293(2): C772 - C782. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Khan, C. Bianchi, M. Ruel, J. Feng, and F. W. Sellke Differential effects on the mesenteric microcirculatory response to vasopressin and phenylephrine after cardiopulmonary bypass J. Thorac. Cardiovasc. Surg., March 1, 2007; 133(3): 682 - 688. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ohtsu, H. Suzuki, H. Nakashima, S. Dhobale, G. D. Frank, E. D. Motley, and S. Eguchi Angiotensin II Signal Transduction Through Small GTP-Binding Proteins: Mechanism and Significance in Vascular Smooth Muscle Cells Hypertension, October 1, 2006; 48(4): 534 - 540. [Full Text] [PDF]
|
|
|
|
 |
|
 K. Budzyn, M. Paull, P. D. Marley, and C. G. Sobey Segmental Differences in the Roles of Rho-Kinase and Protein Kinase C in Mediating Vasoconstriction J. Pharmacol. Exp. Ther., May 1, 2006; 317(2): 791 - 796. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. I. Palen, S. Belmadani, P. A. Lucchesi, and K. Matrougui Role of SHP-1, Kv.1.2, and cGMP in nitric oxide-induced ERK1/2 MAP kinase dephosphorylation in rat vascular smooth muscle cells Cardiovasc Res, November 1, 2005; 68(2): 268 - 277. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Yu, K. Mizumoto, Y. Tokinaga, K. Ogawa, and Y. Hatano The Inhibitory Effects of Sevoflurane on Angiotensin II- Induced, p44/42 Mitogen-Activated Protein Kinase-Mediated Contraction of Rat Aortic Smooth Muscle Anesth. Analg., August 1, 2005; 101(2): 315 - 321. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Xiao, L. D. Longo, and L. Zhang {alpha}1-Adrenoceptor-mediated phosphorylation of MYPT-1 and CPI-17 in the uterine artery: role of ERK/PKC Am J Physiol Heart Circ Physiol, June 1, 2005; 288(6): H2828 - H2835. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. G. Barnes, E. Reiter, J. D. Violin, X.-R. Ren, G. Milligan, and R. J. Lefkowitz {beta}-Arrestin 1 and G{alpha}q/11 Coordinately Activate RhoA and Stress Fiber Formation following Receptor Stimulation J. Biol. Chem., March 4, 2005; 280(9): 8041 - 8050. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Flavahan, S. R. Bailey, W. A. Flavahan, S. Mitra, and S. Flavahan Imaging remodeling of the actin cytoskeleton in vascular smooth muscle cells after mechanosensitive arteriolar constriction Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H660 - H669. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Lucchesi, A. Sabri, S. Belmadani, and K. Matrougui Involvement of Metalloproteinases 2/9 in Epidermal Growth Factor Receptor Transactivation in Pressure-Induced Myogenic Tone in Mouse Mesenteric Resistance Arteries Circulation, December 7, 2004; 110(23): 3587 - 3593. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Roberts The Role of Rho Kinase and Extracellular Regulated Kinase-Mitogen-Activated Protein Kinase in {alpha}2-Adrenoceptor-Mediated Vasoconstriction in the Porcine Palmar Lateral Vein J. Pharmacol. Exp. Ther., November 1, 2004; 311(2): 742 - 747. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Jin, Z. Ying, and R. C. Webb Activation of Rho/Rho kinase signaling pathway by reactive oxygen species in rat aorta Am J Physiol Heart Circ Physiol, October 1, 2004; 287(4): H1495 - H1500. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Fagan, M. Oka, N. R. Bauer, S. A. Gebb, D. D. Ivy, K. G. Morris, and I. F. McMurtry Attenuation of acute hypoxic pulmonary vasoconstriction and hypoxic pulmonary hypertension in mice by inhibition of Rho-kinase Am J Physiol Lung Cell Mol Physiol, October 1, 2004; 287(4): L656 - L664. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Cohen and J. M. Friedman Leptin and the Control of Metabolism: Role for Stearoyl-CoA Desaturase-1 (SCD-1) J. Nutr., September 1, 2004; 134(9): 2455S - 2463S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Herrera, E. C. Martinez, and C. Y. Seow Electron microscopic study of actin polymerization in airway smooth muscle Am J Physiol Lung Cell Mol Physiol, June 1, 2004; 286(6): L1161 - L1168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. E. Spurrell, T. V. Murphy, and M. A. Hill Intraluminal pressure stimulates MAPK phosphorylation in arterioles: temporal dissociation from myogenic contractile response Am J Physiol Heart Circ Physiol, October 1, 2003; 285(4): H1764 - H1773. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Cavarape, J. Bauer, E. Bartoli, K. Endlich, and N. Parekh Effects of angiotensin II, arginine vasopressin and tromboxane A2 in renal vascular bed: role of rho-kinase Nephrol. Dial. Transplant., September 1, 2003; 18(9): 1764 - 1769. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Galle, A. Mameghani, S.-S. Bolz, S. Gambaryan, M. Gorg, T. Quaschning, U. Raff, H. Barth, S. Seibold, C. Wanner, et al. Oxidized LDL and its Compound Lysophosphatidylcholine Potentiate AngII-Induced Vasoconstriction by Stimulation of RhoA J. Am. Soc. Nephrol., June 1, 2003; 14(6): 1471 - 1479. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zeidan, I. Nordstrom, S. Albinsson, U. Malmqvist, K. Sward, and P. Hellstrand Stretch-induced contractile differentiation of vascular smooth muscle: sensitivity to actin polymerization inhibitors Am J Physiol Cell Physiol, June 1, 2003; 284(6): C1387 - C1396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Cavarape, N. Endlich, R. Assaloni, E. Bartoli, M. Steinhausen, N. Parekh, and K. Endlich Rho-Kinase Inhibition Blunts Renal Vasoconstriction Induced by Distinct Signaling Pathways In Vivo J. Am. Soc. Nephrol., January 1, 2003; 14(1): 37 - 45. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Yan, D. Kim, T. Aizawa, and B. C. Berk Functional Interplay Between Angiotensin II and Nitric Oxide: Cyclic GMP as a Key Mediator Arterioscler Thromb Vasc Biol, January 1, 2003; 23(1): 26 - 36. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Loufrani, Z. Li, B. I. Levy, D. Paulin, and D. Henrion Excessive Microvascular Adaptation to Changes in Blood Flow in Mice Lacking Gene Encoding for Desmin Arterioscler Thromb Vasc Biol, October 1, 2002; 22(10): 1579 - 1584. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. W. Carter, M. Begaye, and N. L. Kanagy Acute and chronic NOS inhibition enhances alpha 2- adrenoreceptor-stimulated RhoA and Rho kinase in rat aorta Am J Physiol Heart Circ Physiol, October 1, 2002; 283(4): H1361 - H1369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kobayashi, S. Nakano, S.-i. Mita, T. Kobayashi, T. Honda, Y. Tsubokou, and H. Matsuoka Involvement of Rho-Kinase Pathway for Angiotensin II-Induced Plasminogen Activator Inhibitor-1 Gene Expression and Cardiovascular Remodeling in Hypertensive Rats J. Pharmacol. Exp. Ther., May 1, 2002; 301(2): 459 - 466. [Abstract] [Full Text] [PDF]
|
|