This Article Full Text Full Text (PDF) Data Supplement Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Asset, G. Articles by Dallongeville, J. Search for Related Content PubMed PubMed Citation Articles by Asset, G. Articles by Dallongeville, J. Related Collections Animal models of human disease Pathophysiology Lipid and lipoprotein metabolism

(Arteriosclerosis, Thrombosis, and Vascular Biology. 2001;21:401.)
© 2001 American Heart Association, Inc.

Atherosclerosis and Lipoproteins

Lack of Triglyceride-Lowering Properties of Fish Oil in Apolipoprotein E–Deficient Mice

G. Asset; E. Baugé; J. C. Fruchart; J. Dallongeville

From INSERM U-325 (G.A., E.B., J.C.F.) and INSERM U-508 (J.D.), Lille, France; and Département d’Athérosclérose (G.A., E.B., J.C.F., J.D.), Institut Pasteur de Lille, Lille, France.

Correspondence to Dr Jean Dallongeville, Département d’Athérosclérose, Institut Pasteur de Lille, 1 rue du Professeur Calmette, 59019 Lille Cedex, France. E-mail jean.dallongeville{at}pasteur-lille.fr

Abstract—Fish oil is a potent triglyceride (TG)-lowering agent in humans. The goal of the present study was to assess the contribution of decreased triglyceride synthesis and of apoE in mediation of the triglyceride-lowering effect of fish oil. To this end, apoE-deficient mice and wild-type control mice were supplemented with either coconut oil, sunflower oil, or fish oil (20% wt/wt) for 2 weeks. Compared with coconut oil and sunflower oil, fish oil reduced the concentrations of cholesterol and triglycerides in the wild-type mice, whereas it had no effect on cholesterol concentration and it had a triglyceride–raising effect in apoE-deficient mice. The latter was due to increased triglyceride concentrations in the d<1.019 g/mL plasma density fraction. In apoE-deficient mice, but not in wild-type mice, the postprandial triglyceride area under the curve was higher after an intragastric load of fish oil than after a sunflower oil load. These data indicate an impairment of triglyceride metabolism in the fish oil–fed apoE-deficient mice. Compared with coconut oil and sunflower oil, fish oil lowered triglyceride production rates measured with the Triton method in both wild-type (P<0.0001) and apoE-deficient mice (P<0.0001). Similarly, in vitro lipoprotein lipase–mediated lipolysis of VLDL was lowered in the fish oil–fed wild-type and apoE-deficient mice, suggesting an alteration in VLDL lipolysis independent of the mice genotype. In conclusion, fish oil does not decrease triglyceride concentrations in apoE-deficient mice despite reducing triglyceride production rates, suggesting that decreased triglyceride synthesis is not sufficient to lower triglyceride concentrations in mice. ApoE appears to be necessary for fish oil to lower plasma triglyceride concentrations, indicating a critical role of apoE in this process.

Key Words: apolipoprotein E • triglycerides • fish oil • diet • lipoprotein • VLDL • lipoprotein lipase • atherosclerosis • nutrition

This article has been cited by other articles:

				M. J Caslake, E. A Miles, B. M Kofler, G. Lietz, P. Curtis, C. K Armah, A. C Kimber, J. P Grew, L. Farrell, J. Stannard, et al. Effect of sex and genotype on cardiovascular biomarker response to fish oils: the FINGEN Study Am. J. Clinical Nutrition, September 1, 2008; 88(3): 618 - 629. [Abstract] [Full Text] [PDF]

				H.-H. Wang, T.-M. Hung, J. Wei, and A.-N. Chiang Fish oil increases antioxidant enzyme activities in macrophages and reduces atherosclerotic lesions in apoE-knockout mice Cardiovasc Res, January 1, 2004; 61(1): 169 - 176. [Abstract] [Full Text] [PDF]

				C. Vasandani, A. I. Kafrouni, A. Caronna, Y. Bashmakov, M. Gotthardt, J. D. Horton, and D. K. Spady Upregulation of hepatic LDL transport by n-3 fatty acids in LDL receptor knockout mice J. Lipid Res., May 1, 2002; 43(5): 772 - 784. [Abstract] [Full Text] [PDF]