Arteriosclerosis, Thrombosis, and Vascular Biology. 2000;20:1903-1911
© 2000 American Heart Association, Inc.
Vascular Biology |
Molecular Characterization and Localization of the NAD(P)H Oxidase Components gp91-phox and p22-phox in Endothelial Cells
From the Departments of Cardiology, GKT School of Medicine (U.B., A.M.S.), King’s College, London, and the University of Wales College of Medicine (L.B.), Cardiff, UK.
Correspondence to Professor Ajay M. Shah, Department of Cardiology, GKT School of Medicine, King’s College London, Bessemer Road, London SE5 9PJ, UK. E-mail ajay.shah{at}kcl.ac.uk
Abstract—The production of reactive oxygen species (ROS) within endothelial cells may have several effects, including alterations in the activity of paracrine factors, gene expression, apoptosis, and cellular injury. Recent studies indicate that a phagocyte-type NAD(P)H oxidase is a major source of endothelial ROS. In contrast to the high-output phagocytic oxidase, the endothelial enzyme has much lower biochemical activity and a different substrate specificity (NADH>NADPH). In the present study, we (1) cloned and characterized the cDNA and predicted amino acid structures of the 2 major subunits of rat coronary microvascular endothelial cell NAD(P)H oxidase, gp91-phox and p22-phox; (2) undertook a detailed comparison with phagocytic NADPH oxidase sequences; and (3) studied the subcellular location of these subunits in endothelial cells. Although these studies revealed an overall high degree of homology (>90%) between the endothelial and phagocytic oxidase subunits, the endothelial gp91-phox sequence has potentially important differences in a putative NADPH-binding domain and in putative glycosylation sites. In addition, the subcellular location of the endothelial gp91-phox and p22-phox subunits is significantly different from that reported for the neutrophil oxidase, in that they are predominantly intracellular and collocated in the vicinity of the endoplasmic reticulum. This first detailed characterization of gp91-phox and p22-phox structure and location in endothelial cells provides new data that may account, in part, for the differences in function between the phagocytic and endothelial NAD(P)H oxidases.
Key Words: endothelium • NADPH oxidase • reactive oxygen species • cDNA
This article has been cited by other articles:
 |
|
 |
|
  A. S. Leroyer, T. G. Ebrahimian, C. Cochain, A. Recalde, O. Blanc-Brude, B. Mees, J. Vilar, A. Tedgui, B. I. Levy, G. Chimini, et al. Microparticles From Ischemic Muscle Promotes Postnatal Vasculogenesis Circulation, June 2, 2009; 119(21): 2808 - 2817. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Zhang, P. Malik, D. Pandey, S. Gupta, D. Jagnandan, E. B. de Chantemele, B. Banfi, M. B. Marrero, R. D. Rudic, D. W. Stepp, et al. Paradoxical Activation of Endothelial Nitric Oxide Synthase by NADPH Oxidase Arterioscler Thromb Vasc Biol, September 1, 2008; 28(9): 1627 - 1633. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-S. Silvestre, Z. Mallat, A. Tedgui, and B. I. Levy Post-ischaemic neovascularization and inflammation Cardiovasc Res, May 1, 2008; 78(2): 242 - 249. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. Paravicini and R. M. Touyz NADPH Oxidases, Reactive Oxygen Species, and Hypertension: Clinical implications and therapeutic possibilities Diabetes Care, February 1, 2008; 31(Supplement_2): S170 - S180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Datla, H. Peshavariya, G. J. Dusting, K. Mahadev, B. J. Goldstein, and F. Jiang Important Role of Nox4 Type NADPH Oxidase in Angiogenic Responses in Human Microvascular Endothelial Cells In Vitro Arterioscler Thromb Vasc Biol, November 1, 2007; 27(11): 2319 - 2324. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-E. Chow, Y.-C. Hshu, J.-S. Wang, and J.-K. Chen Resveratrol attenuates oxLDL-stimulated NADPH oxidase activity and protects endothelial cells from oxidative functional damages J Appl Physiol, April 1, 2007; 102(4): 1520 - 1527. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Jagnandan, J. E. Church, B. Banfi, D. J. Stuehr, M. B. Marrero, and D. J. R. Fulton Novel Mechanism of Activation of NADPH Oxidase 5: CALCIUM SENSITIZATION VIA PHOSPHORYLATION J. Biol. Chem., March 2, 2007; 282(9): 6494 - 6507. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Szasz, K. Thakali, G. D. Fink, and S. W. Watts A Comparison of Arteries and Veins in Oxidative Stress: Producers, Destroyers, Function, and Disease Experimental Biology and Medicine, January 1, 2007; 232(1): 27 - 37. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Bedard and K.-H. Krause The NOX Family of ROS-Generating NADPH Oxidases: Physiology and Pathophysiology Physiol Rev, January 1, 2007; 87(1): 245 - 313. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Duerrschmidt, C. Stielow, G. Muller, P. J. Pagano, and H. Morawietz NO-mediated regulation of NAD(P)H oxidase by laminar shear stress in human endothelial cells J. Physiol., October 15, 2006; 576(2): 557 - 567. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. G Ebrahimian, C. Heymes, D. You, O. Blanc-Brude, B. Mees, L. Waeckel, M. Duriez, J. Vilar, R. P. Brandes, B. I. Levy, et al. NADPH Oxidase-Derived Overproduction of Reactive Oxygen Species Impairs Postischemic Neovascularization in Mice with Type 1 Diabetes Am. J. Pathol., August 1, 2006; 169(2): 719 - 728. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Moldovan, K. Mythreye, P. J. Goldschmidt-Clermont, and L. L. Satterwhite Reactive oxygen species in vascular endothelial cell motility. Roles of NAD(P)H oxidase and Rac1 Cardiovasc Res, July 15, 2006; 71(2): 236 - 246. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Paravicini and R. M. Touyz Redox signaling in hypertension Cardiovasc Res, July 15, 2006; 71(2): 247 - 258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Anrather, G. Racchumi, and C. Iadecola NF-{kappa}B Regulates Phagocytic NADPH Oxidase by Inducing the Expression of gp91phox J. Biol. Chem., March 3, 2006; 281(9): 5657 - 5667. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ardanaz and P. J. Pagano Hydrogen peroxide as a paracrine vascular mediator: regulation and signaling leading to dysfunction. Experimental Biology and Medicine, March 1, 2006; 231(3): 237 - 251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Guzik, J. Sadowski, B. Guzik, A. Jopek, B. Kapelak, P. Przybylowski, K. Wierzbicki, R. Korbut, D. G. Harrison, and K. M. Channon Coronary Artery Superoxide Production and Nox Isoform Expression in Human Coronary Artery Disease Arterioscler Thromb Vasc Biol, February 1, 2006; 26(2): 333 - 339. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Gulbins and P. L. Li Physiological and pathophysiological aspects of ceramide Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2006; 290(1): R11 - R26. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-S. Zhou, I. H. Schulman, P. J. Pagano, E. A. Jaimes, and L. Raij Reduced NAD(P)H Oxidase in Low Renin Hypertension: Link Among Angiotensin II, Atherogenesis, and Blood Pressure Hypertension, January 1, 2006; 47(1): 81 - 86. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-Y. Park, R. E. Ferrell, J.-J. Park, J. M. Hagberg, D. A. Phares, J. M. Jones, and M. D. Brown NADPH oxidase p22phox gene variants are associated with systemic oxidative stress biomarker responses to exercise training J Appl Physiol, November 1, 2005; 99(5): 1905 - 1911. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Cai Hydrogen peroxide regulation of endothelial function: Origins, mechanisms, and consequences Cardiovasc Res, October 1, 2005; 68(1): 26 - 36. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kinugawa, J. Zhang, E. Messina, E. Walsh, H. Huang, P. M. Kaminski, M. S. Wolin, and T. H. Hintze gp91phox-containing NAD(P)H oxidase mediates attenuation of nitric oxide-dependent control of myocardial oxygen consumption by ANG II Am J Physiol Heart Circ Physiol, August 1, 2005; 289(2): H862 - H867. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Cai NAD(P)H Oxidase-Dependent Self-Propagation of Hydrogen Peroxide and Vascular Disease Circ. Res., April 29, 2005; 96(8): 818 - 822. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-M. Li, L. M. Fan, M. R. Christie, and A. M. Shah Acute Tumor Necrosis Factor Alpha Signaling via NADPH Oxidase in Microvascular Endothelial Cells: Role of p47phox Phosphorylation and Binding to TRAF4 Mol. Cell. Biol., March 15, 2005; 25(6): 2320 - 2330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kazama, J. Anrather, P. Zhou, H. Girouard, K. Frys, T. A. Milner, and C. Iadecola Angiotensin II Impairs Neurovascular Coupling in Neocortex Through NADPH Oxidase-Derived Radicals Circ. Res., November 12, 2004; 95(10): 1019 - 1026. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-M. Li and A. M Shah Endothelial cell superoxide generation: regulation and relevance for cardiovascular pathophysiology Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2004; 287(5): R1014 - R1030. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. Ambasta, P. Kumar, K. K. Griendling, H. H. H. W. Schmidt, R. Busse, and R. P. Brandes Direct Interaction of the Novel Nox Proteins with p22phox Is Required for the Formation of a Functionally Active NADPH Oxidase J. Biol. Chem., October 29, 2004; 279(44): 45935 - 45941. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Guzik, J. Sadowski, B. Kapelak, A. Jopek, P. Rudzinski, R. Pillai, R. Korbut, and K. M. Channon Systemic Regulation of Vascular NAD(P)H Oxidase Activity and Nox Isoform Expression in Human Arteries and Veins Arterioscler Thromb Vasc Biol, September 1, 2004; 24(9): 1614 - 1620. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. D. Hodgkinson, B. A. Millward, and A. G. Demaine Association of the p22phox Component of NAD(P)H Oxidase With Susceptibility to Diabetic Nephropathy in Patients With Type 1 Diabetes Diabetes Care, November 1, 2003; 26(11): 3111 - 3115. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kitada, D. Koya, T. Sugimoto, M. Isono, S.-i. Araki, A. Kashiwagi, and M. Haneda Translocation of Glomerular p47phox and p67phox by Protein Kinase C-{beta} Activation Is Required for Oxidative Stress in Diabetic Nephropathy Diabetes, October 1, 2003; 52(10): 2603 - 2614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. d. C. P Franco, E. H. Akamine, G. S. Di Marco, D. E. Casarini, Z. B Fortes, R. C.A Tostes, M. H. C Carvalho, and D. Nigro NADPH oxidase and enhanced superoxide generation in intrauterine undernourished rats: involvement of the renin-angiotensin system Cardiovasc Res, September 1, 2003; 59(3): 767 - 775. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Lassegue and R. E. Clempus Vascular NAD(P)H oxidases: specific features, expression, and regulation Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2003; 285(2): R277 - R297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-M. Li and A. M. Shah ROS Generation by Nonphagocytic NADPH Oxidase: Potential Relevance in Diabetic Nephropathy J. Am. Soc. Nephrol., August 1, 2003; 14(90003): S221 - 226. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Jacobson, H. M. Dourron, J. Liu, O. A. Carretero, D. J. Reddy, T. Andrzejewski, and P. J. Pagano Novel NAD(P)H Oxidase Inhibitor Suppresses Angioplasty-Induced Superoxide and Neointimal Hyperplasia of Rat Carotid Artery Circ. Res., April 4, 2003; 92(6): 637 - 643. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Siflinger-Birnboim and A. Johnson Protein kinase C modulates pulmonary endothelial permeability: a paradigm for acute lung injury Am J Physiol Lung Cell Mol Physiol, March 1, 2003; 284(3): L435 - L451. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P A J Krijnen, C Meischl, C E Hack, C J L M Meijer, C A Visser, D Roos, and H W M Niessen Increased Nox2 expression in human cardiomyocytes after acute myocardial infarction J. Clin. Pathol., March 1, 2003; 56(3): 194 - 199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ulker, P. P. McKeown, and U. Bayraktutan Vitamins Reverse Endothelial Dysfunction Through Regulation of eNOS and NAD(P)H Oxidase Activities Hypertension, March 1, 2003; 41(3): 534 - 539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Hayaishi-Okano, Y. Yamasaki, Y. Kajimoto, K.'y. Sakamoto, K. Ohtoshi, N. Katakami, D. Kawamori, T. Miyatsuka, M. Hatazaki, Y. Hazama, et al. Association of NAD(P)H Oxidase p22 phox Gene Variation With Advanced Carotid Atherosclerosis in Japanese Type 2 Diabetes Diabetes Care, February 1, 2003; 26(2): 458 - 463. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. X. Zhang, A.-P. Zou, and P.-L. Li Ceramide-induced activation of NADPH oxidase and endothelial dysfunction in small coronary arteries Am J Physiol Heart Circ Physiol, February 1, 2003; 284(2): H605 - H612. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. L. Parinandi, M. A. Kleinberg, P. V. Usatyuk, R. J. Cummings, A. Pennathur, A. J. Cardounel, J. L. Zweier, J. G. N. Garcia, and V. Natarajan Hyperoxia-induced NAD(P)H oxidase activation and regulation by MAP kinases in human lung endothelial cells Am J Physiol Lung Cell Mol Physiol, January 1, 2003; 284(1): L26 - L38. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. J. Forman and M. Torres Reactive Oxygen Species and Cell Signaling: Respiratory Burst in Macrophage Signaling Am. J. Respir. Crit. Care Med., December 15, 2002; 166(12): S4 - 8. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-J. Cheng, Y.-J. Chao, and D. L. Wang Cyclic Strain Activates Redox-sensitive Proline-rich Tyrosine Kinase 2 (PYK2) in Endothelial Cells J. Biol. Chem., December 6, 2002; 277(50): 48152 - 48157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. E. Rey and P. J. Pagano The Reactive Adventitia: Fibroblast Oxidase in Vascular Function Arterioscler Thromb Vasc Biol, December 1, 2002; 22(12): 1962 - 1971. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. E. Rey, X.-C. Li, O. A. Carretero, J. L. Garvin, and P. J. Pagano Perivascular Superoxide Anion Contributes to Impairment of Endothelium-Dependent Relaxation: Role of gp91phox Circulation, November 5, 2002; 106(19): 2497 - 2502. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-M. Li, N. P. Gall, D. J. Grieve, M. Chen, and A. M. Shah Activation of NADPH Oxidase During Progression of Cardiac Hypertrophy to Failure Hypertension, October 1, 2002; 40(4): 477 - 484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Hu, Z.-X. Yu, V. J. Ferrans, K. Takeda, K. Irani, and R. C. Ziegelstein Critical Role of NADPH Oxidase-derived Reactive Oxygen Species in Generating Ca2+ Oscillations in Human Aortic Endothelial Cells Stimulated by Histamine J. Biol. Chem., August 30, 2002; 277(36): 32546 - 32551. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L Van Heerebeek, C Meischl, W Stooker, C J L M Meijer, H W M Niessen, and D Roos NADPH oxidase(s): new source(s) of reactive oxygen species in the vascular system? J. Clin. Pathol., August 1, 2002; 55(8): 561 - 568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. C. Xu, R. F. Wu, Y. Gu, Y.-S. Yang, M.-C. Yang, F. E. Nwariaku, and L. S. Terada Involvement of TRAF4 in Oxidative Activation of c-Jun N-terminal Kinase J. Biol. Chem., July 26, 2002; 277(31): 28051 - 28057. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Krotz, H. Y. Sohn, T. Gloe, S. Zahler, T. Riexinger, T. M. Schiele, B. F. Becker, K. Theisen, V. Klauss, and U. Pohl NAD(P)H oxidase-dependent platelet superoxide anion release increases platelet recruitment Blood, July 18, 2002; 100(3): 917 - 924. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Touyz, X. Chen, F. Tabet, G. Yao, G. He, M. T. Quinn, P. J. Pagano, and E. L. Schiffrin Expression of a Functionally Active gp91phox-Containing Neutrophil-Type NAD(P)H Oxidase in Smooth Muscle Cells From Human Resistance Arteries: Regulation by Angiotensin II Circ. Res., June 14, 2002; 90(11): 1205 - 1213. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Frey, A. Rahman, J. C. Kefer, R. D. Minshall, and A. B. Malik PKC{zeta} Regulates TNF-{alpha}-Induced Activation of NADPH Oxidase in Endothelial Cells Circ. Res., May 17, 2002; 90(9): 1012 - 1019. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Lassegue and K. K. Griendling Out Phoxing the Endothelium: What's Left Without p47? Circ. Res., February 8, 2002; 90(2): 123 - 124. [Full Text] [PDF]
|
|
|
|
|
|
J.-M. Li, A. M. Mullen, S. Yun, F. Wientjes, G. Y. Brouns, A. J. Thrasher, and A. M. Shah Essential Role of the NADPH Oxidase Subunit p47phox in Endothelial Cell Superoxide Production in Response to Phorbol Ester and Tumor Necrosis Factor-{alpha} Circ. Res., February 8, 2002; 90(2): 143 - 150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Bendall, A. C. Cave, C. Heymes, N. Gall, and A. M. Shah Pivotal Role of a gp91phox-Containing NADPH Oxidase in Angiotensin II-Induced Cardiac Hypertrophy in Mice Circulation, January 22, 2002; 105(3): 293 - 296. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. MacCarthy, D. J. Grieve, J.-M. Li, C. Dunster, F. J. Kelly, and A. M. Shah Impaired Endothelial Regulation of Ventricular Relaxation in Cardiac Hypertrophy: Role of Reactive Oxygen Species and NADPH Oxidase Circulation, December 11, 2001; 104(24): 2967 - 2974. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.S. Wung, J.J. Cheng, S.-K. Shyue, and D.L. Wang NO Modulates Monocyte Chemotactic Protein-1 Expression in Endothelial Cells Under Cyclic Strain Arterioscler Thromb Vasc Biol, December 1, 2001; 21(12): 1941 - 1947. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-M. Li and A. M. Shah Differential NADPH- versus NADH-dependent superoxide production by phagocyte-type endothelial cell NADPH oxidase Cardiovasc Res, December 1, 2001; 52(3): 477 - 486. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. H. WAGNER, M. R. SCHROETER, and M. HECKER 17{beta}-Estradiol inhibition of NADPH oxidase expression in human endothelial cells FASEB J, October 1, 2001; 15(12): 2121 - 2130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Coppey, J. S. Gellett, E. P. Davidson, J. A. Dunlap, D. D. Lund, and M. A. Yorek Effect of Antioxidant Treatment of Streptozotocin-Induced Diabetic Rats on Endoneurial Blood Flow, Motor Nerve Conduction Velocity, and Vascular Reactivity of Epineurial Arterioles of the Sciatic Nerve Diabetes, August 1, 2001; 50(8): 1927 - 1937. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. B. Campbell and D. R. Harder Prologue: EDHF-what is it? Am J Physiol Heart Circ Physiol, June 1, 2001; 280(6): H2413 - H2416. [Full Text] [PDF]
|
|
|
|
|
|
K. Y. Stokes, E. C. Clanton, J. M. Russell, C. R. Ross, and D. N. Granger NAD(P)H Oxidase-Derived Superoxide Mediates Hypercholesterolemia-Induced Leukocyte-Endothelial Cell Adhesion Circ. Res., March 16, 2001; 88(5): 499 - 505. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. Whitehead and G. A. FitzGerald Twenty-First Century Phox: Not Yet Ready for Widespread Screening Circulation, January 2, 2001; 103(1): 7 - 9. [Full Text] [PDF]
|
|
|
|
|
|
M. P. Merker, R. D. Bongard, N. J. Kettenhofen, Y. Okamoto, and C. A. Dawson Intracellular redox status affects transplasma membrane electron transport in pulmonary arterial endothelial cells Am J Physiol Lung Cell Mol Physiol, January 1, 2002; 282(1): L36 - L43. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Didion and F. M. Faraci Effects of NADH and NADPH on superoxide levels and cerebral vascular tone Am J Physiol Heart Circ Physiol, February 1, 2002; 282(2): H688 - H695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-M. Li, A. M. Mullen, S. Yun, F. Wientjes, G. Y. Brouns, A. J. Thrasher, and A. M. Shah Essential Role of the NADPH Oxidase Subunit p47phox in Endothelial Cell Superoxide Production in Response to Phorbol Ester and Tumor Necrosis Factor-{alpha} Circ. Res., February 8, 2002; 90(2): 143 - 150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. D. Wang, S. Xu, D. G. Johns, Y. Du, M. T. Quinn, A. J. Cayatte, and R. A. Cohen Role of NADPH Oxidase in the Vascular Hypertrophic and Oxidative Stress Response to Angiotensin II in Mice Circ. Res., May 9, 2001; 88(9): 947 - 953. [Abstract] [Full Text] [PDF]
|
|