Arteriosclerosis, Thrombosis, and Vascular Biology. 2000;20:1443-1451
© 2000 American Heart Association, Inc.
Vascular Biology |
Development of Endothelial Cell Lines From Embryonic Stem Cells
A Tool for Studying Genetically Manipulated Endothelial Cells In Vitro
From the Istituto di Ricerche Farmacologiche Mario Negri (G.B., R.S., E.D.), Milan, Italy, and Universita’ degli Studi dell’Insubria (E.D.), Dipartimento di Scienze Cliniche e Biologiche, Facolta’ di Medicina e Chirurgia, Varese, Italy.
Correspondence to Dr Giovanna Balconi, Istituto di Ricerche Farmacologiche "Mario Negri," Via Eritrea 62, 20157 Milan, Italy. E-mail balconi{at}irfmn.mnegri.it
Abstract—Totipotent embryonic stem cells can be induced to differentiate to endothelium in vitro. This may be a useful tool for obtaining cultures of genetically manipulated endothelial cells because embryonic stem cells are relatively easy to transfect and are commonly used for gene inactivation experiments in mice. However, embryonic stem cell–derived endothelial cells could not be easily separated from embryoid bodies and maintained in culture. In this study, we describe the isolation and characterization of immortalized endothelial cell lines obtained from embryonic stem cells differentiated in vitro. The cell lines were analyzed for expression of endothelial cell markers, including growth factor receptors and adhesion molecules, and compared with endothelial cells obtained from the yolk sac, the embryo proper, or the heart microcirculation of the adult. We propose that this approach may be useful for obtaining endothelial cells carrying gene mutations that are lethal at very early stages of development.
Key Words: endothelium • embryonic stem cells • vasculogenesis
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