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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2000;20:2329-a.)
© 2000 American Heart Association, Inc.

Letters to the Editor

Oxidized LDL Can Promote Human Monocyte Survival

John A. Hamilton; Genevieve Whitty

Arthritis and Inflammation Research Centre, University of Melbourne, Department of Medicine, The Royal Melbourne Hospital, Parkville, Victoria, Australia, 3050

Wendy Jessup

Heart Research Institute, Camperdown, New South Wales, Australia

To the Editor:

It is likely that in the early stages of atherosclerosis, circulating monocytes migrate into the subendothelial space, where they can mature into foam cells.^{1 2 3 4 5} There is in vivo and in vitro evidence for both foam cell death but also enhanced survival and growth.^{6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22}

Human peripheral blood monocytes (95% pure) were obtained by countercurrent elutriation and usually cultured in minimal essential medium, -modification (-MEM)/1% pooled normal human serum (HS).^{23 24} The number of viable cells was measured by scraping the tissue culture surface and counting them in a hemocytometer with trypan blue exclusion or by propidium iodide staining (flow cytometry). Oxidized LDL (ox-LDL) was prepared as before.¹⁴

The number of viable monocytes declined when they were left untreated or treated with native LDL; this loss was reduced by both ox-LDL and acetylated LDL (ac-LDL; see the Table). A dose response for the ox-LDL effect is provided in the online Figure (please see http://atvb.ahajournals.org) and, as we found before with murine macrophages,¹⁴ doses of ox-LDL 50 µg/mL generally promoted survival; at these survival-inducing doses, the cells spread on the tissue culture surface and remained attached. In contrast, at higher concentrations, viable cell numbers again declined. With different ox-LDL preparations, the effective survival dose response varied to some extent. The ability of ox-LDL to enhance human monocyte survival was confirmed with monocytes from 30 donors. We previously found that prior adherence of the monocytes for a short period under serum-free conditions, followed by culture in 1% HS, improved . . . [Full Text of this Article]

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