© 1999 American Heart Association, Inc.
Atherosclerosis and Lipoproteins |
Chlamydia pneumoniae in Abdominal Aortic Aneurysms
Abundance of Membrane Components in the Absence of Heat Shock Protein 60 and DNA
From the Research Laboratory for Infectious Diseases (A.M., A.v.d.V., J.M.O.) and the Laboratory for Pathology and Immunobiology (P.J.M.R., S.K.G.-P.), National Institute of Public Health and the Environment, Bilthoven; and the Department of Surgery, St. Radboud University Hospital (J.A.v.d.V.), Nijmegen, The Netherlands.
Abstract—In this article, we describe the results of a comparative study for the detection of Chlamydia pneumoniae in abdominal aortic aneurysm specimens of 19 patients through the use of immunocytochemistry (ICC), in situ hybridization (ISH), and polymerase chain reaction (PCR), along with the detection of cytomegalovirus (CMV) and herpes simplex virus (HSV) by ICC and PCR. C pneumoniae–specific membrane protein was detected in specimens of all 19 (100%; 95% confidence interval [CI] 82% to 100%) and of 15 (79%; 95% CI 54% to 94%) patients with monoclonal antibodies RR-402 and TT-401, respectively. Chlamydial lipopolysaccharide was detected in specimens of 15 (79%; 95% CI 54% to 94%) patients when the results of 4 different monoclonal antibodies were combined. Surprisingly, chlamydial heat shock protein 60 was not detected in any of the specimens by ICC. Furthermore, C pneumoniae DNA was not detected by ISH when a C pneumoniae major outer membrane protein gene fragment was used as probe, nor was it reproducibly detected by PCR on extracted DNA. These results may be explained either by different kinetics of degradation of the different components of C pneumoniae after infection of the vessel wall or by the involvement of other Chlamydia-like microorganisms. Coexistence of C pneumoniae antigens and HSV antigens but not CMV antigens was observed in specimens from 10 of 18 (56%; 95% CI 31% to 78%) patients by ICC. CMV and HSV DNAs were not detected by PCR. In conclusion, we have demonstrated the presence of antigens of C pneumoniae in the absence of specific DNA in abdominal aortic aneurysms, suggesting persistence of the antigens rather than a persistent infection.
Key Words: Chlamydia pneumoniae • herpes simplex virus • cytomegalovirus • abdominal aortic aneurysms • atherosclerosis
This article has been cited by other articles:
 |
|
 |
|
  J. Golledge, J. Muller, A. Daugherty, and P. Norman Abdominal Aortic Aneurysm: Pathogenesis and Implications for Management Arterioscler Thromb Vasc Biol, December 1, 2006; 26(12): 2605 - 2613. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Meijer, P. J. M. Roholl, J. M. Ossewaarde, B. Jones, and B. F. Nowak Molecular Evidence for Association of Chlamydiales Bacteria with Epitheliocystis in Leafy Seadragon (Phycodurus eques), Silver Perch (Bidyanus bidyanus), and Barramundi (Lates calcarifer) Appl. Envir. Microbiol., January 1, 2006; 72(1): 284 - 290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Duftner, R. Seiler, P. Klein-Weigel, H. Gobel, C. Goldberger, C. Ihling, G. Fraedrich, and M. Schirmer High Prevalence of Circulating CD4+CD28- T-Cells in Patients With Small Abdominal Aortic Aneurysms Arterioscler Thromb Vasc Biol, July 1, 2005; 25(7): 1347 - 1352. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. F. Berg, B. Maraha, A. van der Zee, S. K. Gielis, P. J. M. Roholl, G.-J. Scheffer, M. F. Peeters, and J. A. J. W. Kluytmans Effect of Clarithromycin Treatment on Chlamydia pneumoniae in Vascular Tissue of Patients with Coronary Artery Disease: a Randomized, Double-Blind, Placebo-Controlled Trial J. Clin. Microbiol., March 1, 2005; 43(3): 1325 - 1329. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Ieven and V. Y. Hoymans Involvement of Chlamydia pneumoniae in Atherosclerosis: More Evidence for Lack of Evidence J. Clin. Microbiol., January 1, 2005; 43(1): 19 - 24. [Full Text] [PDF]
|
|
|
|
|
|
B. Maraha, H. Berg, M. Kerver, S. Kranendonk, J. Hamming, J. Kluytmans, M. Peeters, and A. van der Zee Is the Perceived Association between Chlamydia pneumoniae and Vascular Diseases Biased by Methodology? J. Clin. Microbiol., September 1, 2004; 42(9): 3937 - 3941. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Netea, B. J. Kullberg, L. E. H. Jacobs, T. J. G. Verver-Jansen, J. van der Ven-Jongekrijg, J. M. D. Galama, A. F. H. Stalenhoef, C. A. Dinarello, and J. W. M. Van der Meer Chlamydia pneumoniae Stimulates IFN-{gamma} Synthesis through MyD88-Dependent, TLR2- and TLR4-Independent Induction of IL-18 Release J. Immunol., July 15, 2004; 173(2): 1477 - 1482. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Y. Hoymans, J. M. Bosmans, D. Ursi, W. Martinet, F. L. Wuyts, E. Van Marck, M. Altwegg, C. J. Vrints, and M. M. Ieven Immunohistostaining Assays for Detection of Chlamydia pneumoniae in Atherosclerotic Arteries Indicate Cross-Reactions with Nonchlamydial Plaque Constituents J. Clin. Microbiol., July 1, 2004; 42(7): 3219 - 3224. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Li, Z. Pan, Y. Ji, M. Sheppard, D. J. Jeffries, L. C. Archard, and H. Zhang Herpes Simplex Virus Type 1 Infection Associated with Atrial Myxoma Am. J. Pathol., December 1, 2003; 163(6): 2407 - 2412. [Abstract] [Full Text]
|
|
|
|
 |
|
 S Cagli, N Oktar, T Dalbasti, S Erensoy, N Ozdamar, S Goksel, A Sayiner, and A Bilgic Failure to detect Chlamydia pneumoniae DNA in cerebral aneursymal sac tissue with two different polymerase chain reaction methods J. Neurol. Neurosurg. Psychiatry, June 1, 2003; 74(6): 756 - 759. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. V. Kalayoglu, P. Libby, and G. I. Byrne Chlamydia pneumoniae as an Emerging Risk Factor in Cardiovascular Disease JAMA, December 4, 2002; 288(21): 2724 - 2731. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Apfalter, O. Assadian, F. Blasi, J. Boman, C. A. Gaydos, M. Kundi, A. Makristathis, M. Nehr, M. L. Rotter, and A. M. Hirschl Reliability of Nested PCR for Detection of Chlamydia pneumoniae DNA in Atheromas: Results from a Multicenter Study Applying Standardized Protocols J. Clin. Microbiol., December 1, 2002; 40(12): 4428 - 4434. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Vink, A. H. Schoneveld, J. J. van der Meer, B. J. van Middelaar, J. P.G. Sluijter, M. B. Smeets, P. H.A. Quax, S. K. Lim, C. Borst, G. Pasterkamp, et al. In Vivo Evidence for a Role of Toll-Like Receptor 4 in the Development of Intimal Lesions Circulation, October 8, 2002; 106(15): 1985 - 1990. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. M. Roholl, A. Herrewegh, D. van Soolingen, L. A. Sechi, M. Mura, S. Zanetti, F. Tanda, A. Lissia, and G. Fadda Positive IS900 In Situ Hybridization Signals as Evidence for Role of Mycobacterium avium subsp. paratuberculosis in Etiology of Crohn's Disease J. Clin. Microbiol., August 1, 2002; 40(8): 3112 - 3113. [Full Text] [PDF]
|
|
|
|
 |
|
 M. VALASSINA, L. MIGLIORINI, A. SANSONI, G. SANI, D. CORSARO, M. G. CUSI, P. E. VALENSIN, and C. CELLESI Search for Chlamydia pneumoniae genes and their expression in atherosclerotic plaques of carotid arteries J. Med. Microbiol., March 1, 2001; 50(3): 228 - 232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Apfalter, F. Blasi, J. Boman, C. A. Gaydos, M. Kundi, M. Maass, A. Makristathis, A. Meijer, R. Nadrchal, K. Persson, et al. Multicenter Comparison Trial of DNA Extraction Methods and PCR Assays for Detection of Chlamydia pneumoniae in Endarterectomy Specimens J. Clin. Microbiol., February 1, 2001; 39(2): 519 - 524. [Abstract] [Full Text]
|
|
|
|
 |
|
 A Meijer, P J M Roholl, S K Gielis-Proper, Y F Meulenberg, and J M Ossewaarde Chlamydia pneumoniae in vitro and in vivo: a critical evaluation of in situ detection methods J. Clin. Pathol., December 1, 2000; 53(12): 904 - 910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A Meijer, P J M Roholl, S K Gielis-Proper, and J M Ossewaarde Chlamydia pneumoniae antigens, rather than viable bacteria, persist in atherosclerotic lesions J. Clin. Pathol., December 1, 2000; 53(12): 911 - 916. [Abstract] [Full Text] [PDF]
|
|