© 1999 American Heart Association, Inc.
Original Contributions |
Autoantibodies Against Oxidized Low Density Lipoprotein in Patients With Angiographically Verified Coronary Artery Disease
From the Department of Medical Biochemistry, University of Tampere Medical School (T.L., S.L., O.J., T.N.), Department of Clinical Chemistry, Tampere University Hospital (T.L., S.L., T.S., H.J., T.K.), Department of Internal Medicine, Tampere City Hospital (M.N.), Tampere; and the A. I. Virtanen Institute (J.S.L., S.Y.-H.) and Department of Medicine (S.Y., J.S.L.), University of Kuopio, Kuopio, Finland.
Correspondence to Terho Lehtimäki, MD, PhD, Tampere University Hospital, Department of Clinical Chemistry, Laboratory of Atherosclerosis Genetics, PO Box 2000, FIN-33101 Tampere, Finland. E-mail terho.lehtimaki{at}tays.fi
Abstract—Oxidation of low
density lipoproteins (LDL) obviously plays an important role in the
pathogenesis of atherosclerosis. The purpose of the
study was to determine whether antibodies against oxidized LDL are
associated with coronary artery disease (CAD). We determined
the serum levels of antibodies against copper-oxidized LDL by
enzyme-linked immunosorbent assay in 58 patients with angiographically
verified CAD and 34 controls without CAD. The mean antibody level,
expressed in optical density units, was significantly higher in
patients than in controls (0.150±0.088 versus 0.094±0.054,
respectively; P=0.00089). In logistic regression
analysis, high antibody level against oxidized LDL was
associated significantly with CAD (P=0.0114),
independent of age (P=0.00137), gender
(P=0.0021), body mass index (P=0.5947),
triglyceride concentration (P=0.9813), and
total cholesterol–high density lipoprotein (HDL)
cholesterol (P=0.0080) group. Similar
analysis in nondiabetic subjects (n=79) and in men only (n=75)
showed analogous results, with only minor changes in P
values. The antibody level against oxidized LDL differed significantly
between nonsmokers and smokers in CAD patients
(P<0.00197) but not in controls (P=NS).
In addition, the antibody level against oxidized LDL differed
significantly between nonsmokers and smokers in subjects with low HDL
cholesterol (
0.9 mmol/L) but not in subjects with
high HDL cholesterol (>0.9 mmol/L). In conclusion,
elevated levels of antibodies against oxidized LDL were associated with
CAD. The data suggest that oxidized LDL plays a role in the
pathogenesis of atherosclerosis and suggest a
protective function for HDL against LDL oxidation.
Key Words: autoantibodies • coronary artery disease • low density lipoproteins • oxidized lipoproteins
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