Arteriosclerosis, Thrombosis, and Vascular Biology. 1997;17:898-904
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
© 1997 American Heart Association, Inc.
Articles |
Adenovirus-Mediated Transfer of a Dominant-Negative H-ras Suppresses Neointimal Formation in Balloon-Injured Arteries In Vivo
From the Molecular Cardiology Unit, Department of Cardiology, Kyushu University School of Medicine (H.U., H.Y., J.-J.L., A.T.), and the Department of Dental Anesthesiology, Kyushu University School of Dentistry (S-i.I.), Fukuoka, Japan.
Correspondence to Hikaru Ueno, MD, PhD, Department of Cardiology, Kyushu University School of Medicine, Fukuoka, 812-82 Japan. E-mail ueno{at}cardiol.med.kyushu-u.ac.jp
Abstract Abnormal migration and proliferation of arterial smooth muscle cells may be a central event in inflammatory proliferative arterial diseases such as atherosclerosis and restenosis after angioplasty. The proto-oncogene c-H-ras is considered to be a key transducer in various growth-signaling events. We constructed an adenoviral vector (AdexCAHRasY57) expressing a potent dominant-negative mutated form of c-H-ras in which tyrosine replaces aspartic acid at residue 57. Infection of smooth muscle cells with AdexCAHRasY57 produced a large quantity of H-ras-p21, completely inhibited serum-stimulated activation of mitogen-activated protein kinase, and abolished the DNA synthesis in response to serum mitogens. However, a surge of intracellular Ca2+ concentration in response to platelet-derived growth factor was not affected, suggesting that some cellular functions were preserved. When we applied AdexCAHRasY57 into balloon-injured rat carotid arteries from inside the lumen, neointimal formation was significantly reduced (neointima/media ratio: 0.28) compared with that (1.50) in arteries treated with either injury alone or injury and infection with a control adenovirus, AdexCALacZ, expressing bacterial ß-galactosidase. Our results suggest that adenovirus-mediated arterial transfer of dominant-negative H-ras may be a practical form of effective molecular intervention for proliferative arterial diseases.
Key Words: adenoviral transfer • neointima • balloon injury
This article has been cited by other articles:
 |
|
 |
|
  K. S. Ramos H-RAS Controls Phenotypic Profiles of Vascular Smooth Muscle Cells and the Pathogenesis of Vascular Proliferative Disorders Circ. Res., May 22, 2009; 104(10): 1139 - 1141. [Full Text] [PDF]
|
|
|
|
|
|
D. G. Sedding, M. Trobs, F. Reich, G. Walker, L. Fink, W. Haberbosch, W. Rau, H. Tillmanns, K. T. Preissner, R. M. Bohle, et al. 3-Deazaadenosine Prevents Smooth Muscle Cell Proliferation and Neointima Formation by Interfering With Ras Signaling Circ. Res., May 22, 2009; 104(10): 1192 - 1200. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Lasater, W. K. Bessler, L. E. Mead, W. E. Horn, D. W. Clapp, S. J. Conway, D. A. Ingram, and F. Li Nf1+/- mice have increased neointima formation via hyperactivation of a Gleevec sensitive molecular pathway Hum. Mol. Genet., August 1, 2008; 17(15): 2336 - 2344. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sugita, H. Sugita, and M. Kaneki Farnesyltransferase Inhibitor, Manumycin A, Prevents Atherosclerosis Development and Reduces Oxidative Stress in Apolipoprotein E-Deficient Mice Arterioscler Thromb Vasc Biol, June 1, 2007; 27(6): 1390 - 1395. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Li, A. M. Munchhof, H. A. White, L. E. Mead, T. R. Krier, A. Fenoglio, S. Chen, X. Wu, S. Cai, F.-C. Yang, et al. Neurofibromin is a novel regulator of RAS-induced signals in primary vascular smooth muscle cells Hum. Mol. Genet., June 1, 2006; 15(11): 1921 - 1930. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Tamaoki, K. Isono, K. Takeyama, E. Tagaya, J. Nakata, and A. Nagai Ultrafine carbon black particles stimulate proliferation of human airway epithelium via EGF receptor-mediated signaling pathway Am J Physiol Lung Cell Mol Physiol, December 1, 2004; 287(6): L1127 - L1133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. George, J. Sack, I. Barshack, P. Keren, I. Goldberg, R. Haklai, G. Elad-Sfadia, Y. Kloog, and G. Keren Inhibition of Intimal Thickening in the Rat Carotid Artery Injury Model by a Nontoxic Ras Inhibitor Arterioscler Thromb Vasc Biol, February 1, 2004; 24(2): 363 - 368. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. I. Willis, D. Pierre-Paul, B. E. Sumpio, and V. Gahtan Vascular Smooth Muscle Cell Migration: Current Research and Clinical Implications Vascular and Endovascular Surgery, January 1, 2004; 38(1): 11 - 23. [Abstract] [PDF]
|
|
|
|
|
|
S. Zhang, J. Ren, M. F. Khan, A. M. Cheng, D. Abendschein, and A. J. Muslin Grb2 Is Required for the Development of Neointima in Response to Vascular Injury Arterioscler Thromb Vasc Biol, October 1, 2003; 23(10): 1788 - 1793. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. George, A. Afek, P. Keren, I. Herz, I. Goldberg, R. Haklai, Y. Kloog, and G. Keren Functional Inhibition of Ras by S-trans,trans-Farnesyl Thiosalicylic Acid Attenuates Atherosclerosis in Apolipoprotein E Knockout Mice Circulation, May 21, 2002; 105(20): 2416 - 2422. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. A. Franch, X. Wang, S. Sooparb, N. S. Brown, and J. Du Phosphatidylinositol 3-Kinase Activity Is Required for Epidermal Growth Factor to Suppress Proteolysis J. Am. Soc. Nephrol., April 1, 2002; 13(4): 903 - 909. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Work, A. R. McPhaden, N. J. Pyne, S. Pyne, R. M. Wadsworth, and C. L. Wainwright Short-Term Local Delivery of an Inhibitor of Ras Farnesyltransferase Prevents Neointima Formation In Vivo After Porcine Coronary Balloon Angioplasty Circulation, September 25, 2001; 104(13): 1538 - 1543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Che, J.-i. Abe, M. Yoshizumi, Q. Huang, M. Glassman, S. Ohta, M. G. Melaragno, V. Poppa, C. Yan, N. Lerner-Marmarosh, et al. p160 Bcr Mediates Platelet-Derived Growth Factor Activation of Extracellular Signal-Regulated Kinase in Vascular Smooth Muscle Cells Circulation, September 18, 2001; 104(12): 1399 - 1406. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Iida, K. Namikawa, H. Kiyama, H. Ueno, S. Nakamura, and S. Hattori Requirement of Ras for the Activation of Mitogen-Activated Protein Kinase by Calcium Influx, cAMP, and Neurotrophin in Hippocampal Neurons J. Neurosci., September 1, 2001; 21(17): 6459 - 6466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Atsuchi, T. Nishida, K. Marutsuka, Y. Asada, Y. Kamikubo, A. Takeshita, and H. Ueno Combination of a Brief Irrigation With Tissue Factor Pathway Inhibitor (TFPI) and Adenovirus-Mediated Local TFPI Gene Transfer Additively Reduces Neointima Formation in Balloon-Injured Rabbit Carotid Arteries Circulation, January 30, 2001; 103(4): 570 - 575. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. K. Kerzee and K. S. Ramos Activation of c-Ha-ras by Benzo(a)pyrene in Vascular Smooth Muscle Cells Involves Redox Stress and Aryl Hydrocarbon Receptor Mol. Pharmacol., July 1, 2000; 58(1): 152 - 158. [Abstract] [Full Text]
|
|
|
|
 |
|
 Y. Eto, H. Shimokawa, J. Hiroki, K. Morishige, T. Kandabashi, Y. Matsumoto, M. Amano, M. Hoshijima, K. Kaibuchi, and A. Takeshita Gene transfer of dominant negative Rho kinase suppresses neointimal formation after balloon injury in pigs Am J Physiol Heart Circ Physiol, June 1, 2000; 278(6): H1744 - H1750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Kibbe, T. R. Billiar, and E. Tzeng Gene Therapy for Restenosis Circ. Res., April 28, 2000; 86(8): 829 - 833. [Full Text] [PDF]
|
|
|
|
|
|
R. Baetta, M. Soma, C. De-Fraja, C. Comparato, C. Teruzzi, L. Magrassi, and E. Cattaneo Upregulation and Activation of Stat6 Precede Vascular Smooth Muscle Cell Proliferation in Carotid Artery Injury Model Arterioscler Thromb Vasc Biol, April 1, 2000; 20(4): 931 - 939. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Morishige, H. Shimokawa, T. Yamawaki, K. Miyata, Y. Eto, T. Kandabashi, K. Yogo, T. Higo, K. Egashira, H. Ueno, et al. Local adenovirus-mediated transfer of C-type natriuretic peptide suppresses vascular remodeling in porcine coronary arteries in vivo J. Am. Coll. Cardiol., March 15, 2000; 35(4): 1040 - 1047. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Iwasaki, S. Eguchi, H. Ueno, F. Marumo, and Y. Hirata Mechanical stretch stimulates growth of vascular smooth muscle cells via epidermal growth factor receptor Am J Physiol Heart Circ Physiol, February 1, 2000; 278(2): H521 - H529. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Indolfi, A. Cioppa, E. Stabile, E. Di Lorenzo, G. Esposito, A. Pisani, A. Leccia, L. Cavuto, A. M. Stingone, A. Chieffo, et al. Effects of hydroxymethylglutaryl coenzyme A reductase inhibitor simvastatin on smooth muscle cell proliferation in vitro and neointimal formation in vivo after vascular injury J. Am. Coll. Cardiol., January 1, 2000; 35(1): 214 - 221. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Eguchi, H. Iwasaki, H. Ueno, G. D. Frank, E. D. Motley, K. Eguchi, F. Marumo, Y. Hirata, and T. Inagami Intracellular Signaling of Angiotensin II-induced p70 S6 Kinase Phosphorylation at Ser411 in Vascular Smooth Muscle Cells. POSSIBLE REQUIREMENT OF EPIDERMAL GROWTH FACTOR RECEPTOR, RAS, EXTRACELLULAR SIGNAL-REGULATED KINASE, AND AKT J. Biol. Chem., December 24, 1999; 274(52): 36843 - 36851. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Iwasaki, S. Eguchi, H. Ueno, F. Marumo, and Y. Hirata Endothelin-Mediated Vascular Growth Requires p42/p44 Mitogen-Activated Protein Kinase and p70 S6 Kinase Cascades via Transactivation of Epidermal Growth Factor Receptor Endocrinology, October 1, 1999; 140(10): 4659 - 4668. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Kusumoto, T. Ogawa, K. Mizumoto, H. Ueno, H. Niiyama, N. Sato, M. Nakamura, and M. Tanaka Adenovirus-mediated p53 Gene Transduction Inhibits Telomerase Activity Independent of Its Effects on Cell Cycle Arrest and Apoptosis in Human Pancreatic Cancer Cells Clin. Cancer Res., August 1, 1999; 5(8): 2140 - 2147. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nishida, H. Ueno, N. Atsuchi, R. Kawano, Y. Asada, Y. Nakahara, Y.-i. Kamikubo, A. Takeshita, and H. Yasui Adenovirus-Mediated Local Expression of Human Tissue Factor Pathway Inhibitor Eliminates Shear Stress–Induced Recurrent Thrombosis in the Injured Carotid Artery of the Rabbit Circ. Res., June 25, 1999; 84(12): 1446 - 1452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Matsumoto, K. Yokote, K. Tamura, M. Takemoto, H. Ueno, Y. Saito, and S. Mori Platelet-derived Growth Factor Activates p38 Mitogen-activated Protein Kinase through a Ras-dependent Pathway That Is Important for Actin Reorganization and Cell Migration J. Biol. Chem., May 14, 1999; 274(20): 13954 - 13960. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. J. Kullo, R. D. Simari, and R. S. Schwartz Vascular Gene Transfer : From Bench to Bedside Arterioscler Thromb Vasc Biol, February 1, 1999; 19(2): 196 - 207. [Full Text] [PDF]
|
|
|
|
 |
|
 J. Heckenkamp and G. M. Lamuraglia Intimal Hyperplasia, Arterial Remodeling, and Restenosis: An Overview Perspectives in Vascular Surgery and Endovascular Therapy, January 1, 1999; 11(2): 71 - 94. [Abstract] [PDF]
|
|
|
|
|
|
S. Baek and K. L. March Gene Therapy for Restenosis : Getting Nearer the Heart of the Matter Circ. Res., February 23, 1998; 82(3): 295 - 305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ueno, A. Haruno, N. Morisaki, M. Furuya, K. Kangawa, A. Takeshita, and Y. Saito Local Expression of C-Type Natriuretic Peptide Markedly Suppresses Neointimal Formation in Rat Injured Arteries Through an Autocrine/Paracrine Loop Circulation, October 7, 1997; 96(7): 2272 - 2279. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Sakaue, W. Ogawa, M. Takata, S. Kuroda, K. Kotani, M. Matsumoto, M. Sakaue, S. Nishio, H. Ueno, and M. Kasuga Phosphoinositide 3-Kinase Is Required for Insulin-Induced but Not for Growth Hormone- or Hyperosmolarity-Induced Glucose Uptake in 3T3-L1 Adipocytes Mol. Endocrinol., September 1, 1997; 11(10): 1552 - 1562. [Abstract] [Full Text]
|
|