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(Arteriosclerosis, Thrombosis, and Vascular Biology. 1997;17:3064-3070.)
© 1997 American Heart Association, Inc.

Articles

Cafestol, the Cholesterol-Raising Factor in Boiled Coffee, Suppresses Bile Acid Synthesis by Downregulation of Cholesterol 7-Hydroxylase and Sterol 27-Hydroxylase in Rat Hepatocytes

Sabine M. Post; Elly C. M. de Wit; ; Hans M. G. Princen.

From the Gaubius Laboratory, TNO-PG, Leiden, The Netherlands (S.M.P., E.C.M.deW., H.M.G.P.)

Correspondence to Dr. Hans M.G. Princen, Gaubius Laboratory, TNO-PG, Zernikedreef 9, 2333 CK, Leiden, The Netherlands, POBox 2215, 2301 CE, Leiden, The Netherlands. E-mail jmg.princen{at}pg.tno.nl

Abstract Consumption of boiled coffee raises serum cholesterol levels in humans. The diterpenes cafestol and kahweol in boiled coffee have been found to be responsible for the increase. To investigate the biochemical background of this effect, we studied the effects of cafestol and a mixture of cafestol/kahweol/isokahweol (48:47:5 w/w) on bile acid synthesis and cholesterol 7-hydroxylase and sterol 27-hydroxylase in cultured rat hepatocytes.

Dose-dependent decreases of bile acid mass production and cholesterol 7-hydroxylase and sterol 27-hydroxylase activity were found, showing a maximal reduction of -91%, -79%, and -49% respectively, at a concentration of 20 µg/mL cafestol. The decrease in 7-hydroxylase and 27-hydroxylase activity paralleled well the suppression of the respective mRNAs, being -79% and -77%, and -49% and -46%, respectively, at 20 µg/mL cafestol. Run-on data showed a reduction in 7-hydroxylase and 27-hydroxylase gene transcriptional activity after incubation with cafestol. The mixture of cafestol/kahweol/isokahweol was less potent in suppression of bile acid synthesis and cholesterol 7-hydroxylase. Cafestol (20 µg/mL) had no effect on lithocholic acid 6ß-hydroxylase mRNA, another enzyme involved in bile acid synthesis. LDL-receptor, HMG-CoA reductase, and HMG-CoA synthase mRNAs were significantly decreased by cafestol (-18%, -20%, and -43%, respectively).

We conclude that cafestol suppresses bile acid synthesis by downregulation of cholesterol 7-hydroxylase and of, to a lesser extent, sterol 27-hydroxylase in cultured rat hepatocytes, whereas kahweol and isokahweol are less active. We suggest that suppression of bile acid synthesis may provide an explanation for the cholesterol-raising effect of cafestol in humans.

Key Words: bile acid synthesis • cholesterol 7-hydroxylase • sterol 27-hydroxylase • rat hepatocytes • cafestol

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