This Article Full Text Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Liinamaa, M. J. Articles by Savolainen, M. J. Search for Related Content PubMed PubMed Citation Articles by Liinamaa, M. J. Articles by Savolainen, M. J.

(Arteriosclerosis, Thrombosis, and Vascular Biology. 1997;17:2940-2947.)
© 1997 American Heart Association, Inc.

Articles

Altered Transfer of Cholesteryl Esters and Phospholipids in Plasma From Alcohol Abusers

M. Johanna Liinamaa; Minna L. Hannuksela; Y. Antero Kesäniemi; ; Markku J. Savolainen

From the Department of Internal Medicine and Biocenter Oulu, University of Oulu, FIN-90220 Oulu, Finland.

Correspondence to Markku Savolainen, MD, PhD, Department of Internal Medicine, University of Oulu, Kajaanintie 50, FIN-90220 Oulu, Finland. E-mail markku.savolainen{at}oulu.fi

Abstract The net mass transfer (NMT) of cholesteryl esters (CEs), triglycerides (TGs), and phospholipids (PLs) between lipoproteins was measured after incubation of fresh plasma for up to 2 hours from 18 male alcohol abusers and 17 male volunteer control subjects. In alcohol abusers the mean value of CE NMT was 3.7 nmol·mL^-1·h^-1 from apolipoprotein B–containing lipoproteins (apoB-containing lipoproteins) to HDL and in control subjects 8.7 nmol · mL^-1 · h^-1 from HDL to apoB-containing lipoproteins. The NMT of PL was higher in alcohol abusers than in control subjects (35.0 vs 11.6 nmol·mL^-1·h^-1 from apoB-containing lipoproteins to HDL, respectively), and plasma PL transfer protein (TP) activity was 33% higher (P<.05) in alcohol abusers than in control subjects. The lack of correlation between the NMTs and CETP and PLTP activities suggests that the NMT could more closely reflect the role of lipoprotein properties in reverse cholesterol transport in vivo, whereas in vitro activities reflect the total capacity of transfer but not its direction. The rate of CE NMT from HDL to apoB-containing lipoproteins was dependent on the VLDL TG concentration. Moreover, at low VLDL TG levels, the increased HDL cholesterol concentration in alcohol abusers reversed the direction of CE NMT. This situation could be reconstructed in the plasma of control subjects by adding autologous HDL or VLDL to mimic the lipoprotein profiles of the alcohol abusers. Addition of VLDL enhanced the CE NMT from HDL to apoB-containing lipoproteins, whereas addition of HDL had an opposite effect, and at higher HDL levels, even reversed the direction of CE NMT. In conclusion, the NMT of CE and PL in alcohol abusers differs from that in control subjects. The concentrations of HDL and VLDL seem to be the major determinants of the direction of CE NMT in alcohol abusers.

Key Words: cholesteryl ester transfer protein • reverse cholesterol transport • phospholipid transfer protein • lipoproteins • lipids

This article has been cited by other articles:

				C. Schafer, A. Parlesak, J. Eckoldt, C. Bode, J. C. Bode, W. Marz, and K. Winkler Beyond HDL-cholesterol increase: phospholipid enrichment and shift from HDL3 to HDL2 in alcohol consumers J. Lipid Res., July 1, 2007; 48(7): 1550 - 1558. [Abstract] [Full Text] [PDF]

				M. J. LIINAMAA, M. L. HANNUKSELA, M. E. RAMET, and M. J. SAVOLAINEN DEFECTIVE GLYCOSYLATION OF CHOLESTERYL ESTER TRANSFER PROTEIN IN PLASMA FROM ALCOHOL ABUSERS Alcohol Alcohol., January 1, 2006; 41(1): 18 - 23. [Abstract] [Full Text] [PDF]

				J. T Salonen Liver damage and protective effect of high density lipoprotein cholesterol BMJ, November 8, 2003; 327(7423): 1082 - 1083. [Full Text] [PDF]

				S. J. Murdoch, S. E. Kahn, J. J. Albers, J. D. Brunzell, and J. Q. Purnell PLTP activity decreases with weight loss: changes in PLTP are associated with changes in subcutaneous fat and FFA but not IAF or insulin sensitivity J. Lipid Res., September 1, 2003; 44(9): 1705 - 1712. [Abstract] [Full Text] [PDF]

				D. Corella, K. Tucker, C. Lahoz, O. Coltell, L A. Cupples, P. W. Wilson, E. J Schaefer, and J. M Ordovas Alcohol drinking determines the effect of the APOE locus on LDL-cholesterol concentrations in men: the Framingham Offspring Study Am. J. Clinical Nutrition, April 1, 2001; 73(4): 736 - 745. [Abstract] [Full Text] [PDF]

				T. Oka, T. Kujiraoka, M. Ito, T. Egashira, S. Takahashi, M. N. Nanjee, N. E. Miller, J. Metso, V. M. Olkkonen, C. Ehnholm, et al. Distribution of phospholipid transfer protein in human plasma: presence of two forms of phospholipid transfer protein, one catalytically active and the other inactive J. Lipid Res., October 1, 2000; 41(10): 1651 - 1657. [Abstract] [Full Text]

				T. Oka, T. Kujiraoka, M. Ito, M. Nagano, M. Ishihara, T. Iwasaki, T. Egashira, N. E. Miller, and H. Hattori Measurement of Human Plasma Phospholipid Transfer Protein by Sandwich ELISA Clin. Chem., September 1, 2000; 46(9): 1357 - 1364. [Abstract] [Full Text] [PDF]