Arteriosclerosis, Thrombosis, and Vascular Biology. 1996;16:600-605
© 1996 American Heart Association, Inc.
Articles |
Lysophosphatidylcholine Potentiates the Mitogenic Activity of Modified LDL for Human Monocyte–Derived Macrophages
From the Departments of Biochemistry (A.M., H.H., S.H.) and Metabolic Medicine (M. Sakai, Y.S., T.M., S.K., M. Shichiri), Kumamoto University School of Medicine, Kumamoto, Japan.
Correspondence to Seikoh Horiuchi, MD, PhD, Department of Biochemistry, Kumamoto University School of Medicine, Honjo 2-2-1, Kumamoto 860, Japan.
Abstract The growth of murine peritoneal macrophages is induced by oxidized LDL (Ox-LDL), and lysophosphatidylcholine (lysoPC) plays an important role in its mitogenic activity. In the present study, Ox-LDL–induced macrophage growth was examined with human monocyte–derived macrophages. The cell growth of human macrophages was significantly induced by Ox-LDL but not by acetylated LDL (acetyl-LDL). The treatment of acetyl-LDL with phospholipase A2, however, led to a marked increase in its mitogenic activity, with a concomitant conversion of 75% of its phospholipids to lysoPC. The growth-stimulating activity became positive only when both acetyl-LDL and lysoPC were coincubated, although neither of them exhibited cell growth–promoting activity. These results suggest that Ox-LDL could stimulate the growth of human monocyte–derived macrophages, and lysoPC may play an essential role in the mitogenic activity of Ox-LDL.
Key Words: human monocyte–derived macrophage • foam cell • oxidized LDL • lysophosphatidylcholine • scavenger receptor
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