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(Arteriosclerosis, Thrombosis, and Vascular Biology. 1996;16:230-235.)
© 1996 American Heart Association, Inc.

Articles

Association of Localized Ca²⁺ Gradients With Redistribution of Glycoprotein IIb-IIIa and F-actin in Activated Human Blood Platelets

Hideo Ariyoshi; Edwin W. Salzman

From the Department of Surgery, Beth Israel Hospital, Harvard Medical School, Boston, Mass.

Correspondence to Edwin W. Salzman, MD, Beth Israel Hospital, 330 Brookline Ave, Boston, MA 02215.

Abstract We monitored the intracellular distribution of ionized free Ca²⁺ concentration ([Ca²⁺]_i) in individual human platelets by digital imaging fluorescence microscopy with fura 2 during platelet activation induced by surface contact or a soluble platelet agonist (thrombin). Contact of platelets with glass resulted in pseudopod formation and spreading, accompanied by a nonuniform rise in [Ca²⁺]_i. The rise in [Ca²⁺]_i was maximal during pseudopod formation. Locally elevated [Ca²⁺]_i was frequently found in pseudopodia and at the edge and core of spread platelets. This pattern was faithfully duplicated by the local pattern of distribution of the cytoskeletal components F-actin, gelsolin, and surface glycoproteins (GP) IIb-IIIa but not by calmodulin. Platelets stimulated by thrombin also showed an inhomogeneous rise in [Ca²⁺]_i, which was well correlated with the staining of F-actin and GPIIb-IIIa. Cytochalasin D, an inhibitor of actin polymerization, inhibited the inhomogeneous increase or redistribution of F-actin and GPIIb-IIIa but did not inhibit the rise in mean [Ca²⁺]_i. These observations suggest that a localized change in [Ca²⁺]_i may be associated with cytoskeletal reorganization and redistribution of GPIIb-IIIa in activated platelets.

Key Words: fluorescence microscopy • calcium • F-actin • integrins

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