This Article Full Text Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fan, Y.-Y. Articles by Chapkin, R. S. Search for Related Content PubMed PubMed Citation Articles by Fan, Y.-Y. Articles by Chapkin, R. S.

(Arteriosclerosis, Thrombosis, and Vascular Biology. 1995;15:1397-1403.)
© 1995 American Heart Association, Inc.

Articles

Dietary -Linolenic Acid Modulates Macrophage–Vascular Smooth Muscle Cell Interactions

Evidence for a Macrophage-Derived Soluble Factor That Downregulates DNA Synthesis in Smooth Muscle Cells

Yang-Yi Fan; Kenneth S. Ramos; Robert S. Chapkin

From the Faculty of Nutrition and Molecular and Cell Biology Group (Y.-Y.F., R.S.C.) and the Department of Veterinary Physiology and Pharmacology (K.S.R.), Texas A&M University, College Station.

Correspondence to Dr Robert S. Chapkin, Faculty of Nutrition, 442 Kleberg Center, Texas A&M University, College Station, TX 77843-2471. E-mail chapkin@zeus.tamu.edu.

Abstract Macrophages and smooth muscle cells (SMCs) are two of the major reactive cell types in atherosclerosis, a disease characterized by uncontrolled proliferation of SMCs. The present study was designed to determine how dietary oils containing -linolenic acid (GLA) (primrose oil [PO]) and long-chain n-3 fatty acids (fish oil) influence the ability of macrophages to modulate SMC DNA synthesis in vitro. Mice were fed one of four diets containing 10% (wt/wt) corn oil (CO), PO, fish oil–CO mix (FC; 9:1, wt/wt), or fish oil–PO mix (FP; 1:3, wt/wt) for 2 weeks. Resident peritoneal macrophages were isolated from these mice and seeded on a semipermeable membrane with a 30-kDa cutoff. Macrophages were preincubated with or without 50 µmol/L indomethacin (a cyclooxygenase inhibitor) or 50 µmol/L L655,238 (a 5-lipoxygenase inhibitor) for 30 minutes and subsequently cocultured with naive murine aortic SMCs grown on culture dishes. DNA synthesis in SMCs and prostaglandin formation in coculture supernatants were measured at the end of a 39-hour incubation period. SMC DNA synthesis was inhibited by 28% and 60% in PO and FP diets containing 10.1% and 8.2% GLA, respectively, relative to the control CO diet containing no GLA or long-chain n-3 fatty acid. A fourfold increase in the levels of PGE₁, a potent antiproliferative eicosanoid derived from GLA, was observed in the PO and FP groups relative to the control CO group. Although PGE₁ levels were not different between the CO and FC dietary groups, 15% inhibition of SMC DNA synthesis, relative to that in mice fed the control CO diet, was observed in mice fed the FC diet containing 13.3% 20:5n-3 and 7.6% 22:6n-3 fatty acids. Macrophage inhibition of SMC DNA synthesis and proliferation in mice consuming GLA-enriched diets was blocked by indomethacin but not by L655,238. Addition of exogenous PGE₁ (100 nmol/L) reversed the effect of indomethacin. In experiments in which mice were fed increasing levels of GLA-containing triglycerides, the ability of macrophages to downregulate SMC proliferation was modulated in a dose-dependent fashion. These data indicate that macrophages isolated from animals consuming diets supplemented with dietary oils containing GLA reduce SMC DNA synthesis and proliferation in a cyclooxygenase-dependent manner and therefore may favorably modulate the atherogenic process.

Key Words: prostaglandin E₁ • primrose oil • fish oil • atherosclerosis • cyclooxygenase

This article has been cited by other articles:

				S. Sengupta, L. A. Sellers, T. Cindrova, J. Skepper, E. Gherardi, R. Sasisekharan, and T.-P. D. Fan Cyclooxygenase-2-selective Nonsteroidal Anti-Inflammatory Drugs Inhibit Hepatocyte Growth Factor/Scatter Factor-induced Angiogenesis Cancer Res., December 1, 2003; 63(23): 8351 - 8359. [Abstract] [Full Text] [PDF]

				Y.-Y. Fan, K. S. Ramos, and R. S. Chapkin Dietary {{gamma}}-Linolenic Acid Suppresses Aortic Smooth Muscle Cell Proliferation and Modifies Atherosclerotic Lesions in Apolipoprotein E Knockout Mice J. Nutr., June 1, 2001; 131(6): 1675 - 1681. [Abstract] [Full Text]

				Y.-Y. Fan and R. S. Chapkin Importance of Dietary gamma -Linolenic Acid in Human Health and Nutrition J. Nutr., September 1, 1998; 128(9): 1411 - 1414. [Abstract] [Full Text]

				Y.-Y. Fan, K. S. Ramos, and R. S. Chapkin Dietary gamma -Linolenic Acid Enhances Mouse Macrophage-Derived Prostaglandin E1 Which Inhibits Vascular Smooth Muscle Cell Proliferation J. Nutr., September 1, 1997; 127(9): 1765 - 1771. [Abstract] [Full Text]