© 1995 American Heart Association, Inc.
Articles |
Evidence for Nonesterified Fatty Acids as Modulators of Neutral Lipid Transfers in Normolipidemic Human Plasma
Presented in part at the 65th Scientific Sessions of the American Heart Association, New Orleans, La, and published in abstract form (Circulation. 1992;86[suppl I]:I-71.)
From the Laboratoire de Biochimie des Lipoprotéines, INSERM CJF 93-10, Faculté de Médecine, Dijon, France.
Correspondence to Laurent Lagrost, Laboratoire de Biochimie Médicale, Hôpital du Bocage, 21034 Dijon, France.
Abstract The relations between the level of plasma
nonesterified fatty acid (NEFA) and both the mass concentration and
activity of the cholesteryl ester transfer protein (CETP) were studied
in fasted normolipidemic subjects. Plasma NEFA correlated positively
with both CETP mass concentration (r=.50; P<.01)
and the transfer of cholesteryl ester from HDL toward plasma VLDL+LDL
(CETHDL
VLDL+LDL activity) (r=.46;
P<.05) but not with the transfer of cholesteryl ester from
LDL toward plasma HDL (CETLDLHDL activity)
(r=-.05; NS). The high binding capacity of
albumin for NEFA was used to investigate whether
lipoprotein-bound NEFAs were implicated in the modulation of the
cholesteryl ester transfer reaction. As compared with nonsupplemented
controls, the addition of an excess of fatty acid–free
albumin (8 g/L) to total normolipidemic plasmas reduced
CETHDLVLDL+LDL activity (18.3±5.5% versus 9.8±3.1%;
P<.0001) but not CETLDLHDL activity
(22.3±4.5% versus 23.3±5.1%; NS). Moreover,
CETHDLVLDL+LDL and CETLDLHDL activities
correlated negatively when measured in native plasma
(r=-.45; P<.05) but positively when measured in
albumin-supplemented plasma (r=.40;
P<.05). In long-term incubation experiments,
lipoprotein-bound NEFA increased the net mass transfer of
cholesteryl esters from HDL toward VLDL+LDL but reduced the net mass
transfer of triglycerides in the opposite direction, from
VLDL+LDL toward HDL. Taken together, data of the present study
brought strong and concordant arguments in favor of a dual effect of
plasma NEFA in modulating both the mass and the activity of CETP in
vivo.
Key Words: plasma • lipoprotein • cholesteryl ester • triglyceride • cholesteryl ester transfer protein
This article has been cited by other articles:
 |
|
 |
|
  R. E. Morton and D. J. Greene CETP and lipid transfer inhibitor protein are uniquely affected by the negative charge density of the lipid and protein domains of LDL J. Lipid Res., December 1, 2003; 44(12): 2287 - 2296. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. J. A. M. Jonkers, A. H. M. Smelt, H. Hattori, L. M. Scheek, T. van Gent, F. H. A. F. de Man, A. van der Laarse, and A. van Tol Decreased PLTP mass but elevated PLTP activity linked to insulin resistance in HTG: effects of bezafibrate therapy J. Lipid Res., August 1, 2003; 44(8): 1462 - 1469. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. V. Sekhar, F. Jahoor, A. C. White, H. J. Pownall, F. Visnegarwala, M. C. Rodriguez-Barradas, M. Sharma, P. J. Reeds, and A. Balasubramanyam Metabolic basis of HIV-lipodystrophy syndrome Am J Physiol Endocrinol Metab, August 1, 2002; 283(2): E332 - E337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Gander, P. Eller, S. Kaser, I. Theurl, D. Walter, T. Sauper, A. Ritsch, J. R. Patsch, and B. Foger Molecular characterization of rabbit phospholipid transfer protein: choroid plexus and ependyma synthesize high levels of phospholipid transfer protein J. Lipid Res., April 1, 2002; 43(4): 636 - 645. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Guerin, W. Le Goff, T. S. Lassel, A. Van Tol, G. Steiner, and M. J. Chapman Proatherogenic Role of Elevated CE Transfer From HDL to VLDL1 and Dense LDL in Type 2 Diabetes : Impact of the Degree of Triglyceridemia Arterioscler Thromb Vasc Biol, February 1, 2001; 21(2): 282 - 288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Stamler, D. Breznan, T. A-M. Neville, F. J. Viau, E. Camlioglu, and D. L. Sparks Phosphatidylinositol promotes cholesterol transport in vivo J. Lipid Res., August 1, 2000; 41(8): 1214 - 1221. [Abstract] [Full Text]
|
|
|
|
|
|
V. Pruneta, T. Pulcini, F. Lalanne, C. Marcais, F. Berthezene, G. Ponsin, and P. Moulin VLDL-bound lipoprotein lipase facilitates the cholesteryl ester transfer protein-mediated transfer of cholesteryl esters from HDL to VLDL J. Lipid Res., December 1, 1999; 40(12): 2333 - 2339. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. J. Pownall, C. M. Ballantyne, K. T. Kimball, S. L. Simpson, D. Yeshurun, and A. M. Gotto Jr Effect of Moderate Alcohol Consumption on Hypertriglyceridemia: A Study in the Fasting State Arch Intern Med, May 10, 1999; 159(9): 981 - 987. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. D. Sniderman, K. Cianflone, P. Arner, L. K. M. Summers, and K. N. Frayn The Adipocyte, Fatty Acid Trapping, and Atherogenesis Arterioscler Thromb Vasc Biol, February 1, 1998; 18(2): 147 - 151. [Full Text] [PDF]
|
|
|
|
|
|
S. Braschi, D. Masson, G. Rostoker, E. Florentin, A. Athias, C. Martin, B. Jacotot, P. Gambert, C. Lallemant, and L. Lagrost Role of Lipoprotein-Bound NEFAs in Enhancing the Specific Activity of Plasma CETP in the Nephrotic Syndrome Arterioscler Thromb Vasc Biol, November 1, 1997; 17(11): 2559 - 2567. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. Lagrost, A. Athias, B. Herbeth, V. Guyard-Dangremont, Y. Artur, F. Paille, P. Gambert, and C. Lallemant Opposite Effects of Cholesteryl Ester Transfer Protein and Phospholipid Transfer Protein on the Size Distribution of Plasma High Density Lipoproteins. PHYSIOLOGICAL RELEVANCE IN ALCOHOLIC PATIENTS J. Biol. Chem., August 9, 1996; 271(32): 19058 - 19065. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Braschi, L. Lagrost, E. Florentin, C. Martin, A. Athias, P. Gambert, M. Krempf, C. Lallemant, and B. Jacotot Increased Cholesteryl Ester Transfer Activity in Plasma From Analbuminemic Patients Arterioscler Thromb Vasc Biol, March 1, 1996; 16(3): 441 - 449. [Abstract] [Full Text]
|
|