This Article Full Text Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ilmonen, M. Articles by Tikkanen, M. J. Search for Related Content PubMed PubMed Citation Articles by Ilmonen, M. Articles by Tikkanen, M. J.

(Arteriosclerosis, Thrombosis, and Vascular Biology. 1995;15:1287-1293.)
© 1995 American Heart Association, Inc.

Articles

Two New Immunogenetic Polymorphisms of the ApoB Gene and Their Effect on Serum Lipid Levels and Responses to Changes in Dietary Fat Intake

Marja Ilmonen; Tiina Heliö; René Bütler; Aarno Palotie; Pirjo Pietinen; Jussi K. Huttunen; Matti J. Tikkanen

From the Department of Medicine, Division of Cardiology, Helsinki University Central Hospital (M.I., T.H., M.J.T.), the Department of Clinical Chemistry, University of Helsinki (A.P.), the National Public Health Institute (P.P., J.K.H.), Helsinki, Finland, and the Blood Transfusion Service of the Swiss Red Cross, Bern, Switzerland (R.B.).

Correspondence to Marja Ilmonen, MD, Helsinki University Central Hospital, Department of Medicine, Laboratory of Biochemistry L222, Haartmaninkatu 4, FIN-00290 Helsinki, Finland.

Abstract In previous studies, apoB polymorphisms have been shown to modify serum lipid responses to changes in dietary fat intake. The functionally important apoB DNA change or changes underlying these effects have, however, remained unknown. Using a single-strand conformation polymorphism analysis–based screening method, we identified two previously unreported apoB polymorphisms located close to each other in the 5' region of apoB gene exon 26. This DNA segment corresponds to the binding site of monoclonal anti-apoB antibody D7.2. The two AG changes at apoB cDNA nucleotides 5869 and 5896 produced an AsnSer change at amino acid 1887 and a HisArg change at amino acid 1896. In the Finnish population, allele frequencies of the rare alleles of the apoB 1887 (AsnSer) and apoB 1896 (HisArg) polymorphisms were .02 and .11, respectively. Both polymorphisms were shown to have an independent effect on the binding affinity of LDL with monoclonal antibody D7.2. The effect of these polymorphisms on serum lipid levels and responses to changes in dietary fat intake in 102 healthy free-living subjects was assessed. The apoB 1896 Arg allele was associated with a higher serum LDL cholesterol level during a low-fat, low-cholesterol diet in men.

Key Words: apoB gene polymorphisms • apoB antigenic polymorphisms • low-fat, low-cholesterol diet

This article has been cited by other articles:

				L. F Masson, G. McNeill, and A. Avenell Genetic variation and the lipid response to dietary intervention: a systematic review Am. J. Clinical Nutrition, May 1, 2003; 77(5): 1098 - 1111. [Abstract] [Full Text] [PDF]

				L. Beghin, N. Duhal, P. Poulain, P. Hauw, B. Lacroix, J.-M. Lecerf, J.-P. Bonte, J.-C. Fruchart, and G. Luc Measurement of apolipoprotein B concentration in plasma lipoproteins by combining selective precipitation and mass spectrometry J. Lipid Res., July 1, 2000; 41(7): 1172 - 1176. [Abstract] [Full Text]

				B. Vedie, X. Jeunemaitre, J. L. Megnien, I. Myara, H. Trebeden, A. Simon, and N. Moatti Charge Heterogeneity of LDL in Asymptomatic Hypercholesterolemic Men Is Related to Lipid Parameters and Variations in the ApoB and CIII Genes Arterioscler Thromb Vasc Biol, November 1, 1998; 18(11): 1780 - 1789. [Abstract] [Full Text] [PDF]