This Article Full Text Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ishii, I. Articles by Hirose, S. Search for Related Content PubMed PubMed Citation Articles by Ishii, I. Articles by Hirose, S. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB CHOLESTEROL

(Arteriosclerosis, Thrombosis, and Vascular Biology. 1995;15:1189-1194.)
© 1995 American Heart Association, Inc.

Articles

Genetic Differences of Lipid Metabolism in Macrophages From C57BL/6J and C3H/HeN Mice

Itsuko Ishii; Yasuhiko Ito; Nobuhiro Morisaki; Yasushi Saito; Seiyu Hirose

From the Department of Biochemistry, Faculty of Pharmaceutical Science (I.I., Y.I., S.H.), and the Second Department of Internal Medicine, School of Medicine (N.M.), Chiba University, and the Department of Laboratory Medicine, Yamagata University (Y.S.), Japan.

Correspondence to Itsuko Ishii, Department of Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Inage-ku 1-33, Yayoi-cho, Chiba, 263, Japan.

Abstract Cholesterol metabolism in macrophages from atherosclerosis-prone C57BL/6J mice was compared with that in macrophages from atherosclerosis-resistant C3H/HeN mice. Plasma total cholesterol levels of both types of mice were significantly increased, but HDL cholesterol level was increased only in C3H/HeN mice when a high-cholesterol diet (1% cholesterol) was fed for 5 weeks. After incubation of macrophages from male and female mice on the high-cholesterol diet with ß-VLDL for 24 hours, cholesterol content in macrophages from C57BL/6J was approximately 1.5- to 2.0-fold higher than in those from C3H/HeN mice. [³H]Cholesterol oleate–ß-VLDL incorporation into macrophages from C57BL/6J mice on the high-cholesterol diet was greater than incorporation into those from C3H/HeN mice. The release of [³H]cholesterol from macrophages from C57BL/6J mice on the high-cholesterol diet was one seventh that from macrophages from C57BL/6J mice on the basal diet or that from macrophages from C3H/HeN mice on the basal or high-cholesterol diet. Acid cholesterol esterase activity was almost the same in macrophages from any group. Acyl CoA:cholesterol acyltransferase activity in macrophages from C57BL/6J mice on the high-cholesterol diet increased compared with that from macrophages from C57BL/6J mice on the normal diet. Neutral cholesterol esterase activity in macrophages from C57BL/6J mice was about half of that in macrophages from C3H/HeN mice independent of the type of diet. There were no sex differences in these metabolisms. Considered with our previous data, these results suggested that a high-cholesterol diet may cause metabolic changes to accumulate cholesterol ester in macrophages from C57BL/6J mice in accordance with genetic abnormalities.

Key Words: high-cholesterol diet • neutral cholesterol esterase • C57BL/6J mouse • macrophage • acyl CoA:cholesterol acyltransferase

This article has been cited by other articles:

				S. Ghosh, R. W. St. Clair, and L. L. Rudel Mobilization of cytoplasmic CE droplets by overexpression of human macrophage cholesteryl ester hydrolase J. Lipid Res., October 1, 2003; 44(10): 1833 - 1840. [Abstract] [Full Text] [PDF]

				L. O’Rourke, S. J. Yeaman, and P. R. Shepherd Insulin and Leptin Acutely Regulate Cholesterol Ester Metabolism in Macrophages by Novel Signaling Pathways Diabetes, May 1, 2001; 50(5): 955 - 961. [Abstract] [Full Text]

				G. Friedman, A. Ben-Yehuda, Y. Dabach, G. Hollander, S. Babaey, M. Ben-Naim, O. Stein, and Y. Stein Macrophage Cholesterol Metabolism, Apolipoprotein E, and Scavenger Receptor AI/II mRNA in Atherosclerosis-Susceptible and -Resistant Mice Arterioscler Thromb Vasc Biol, November 1, 2000; 20(11): 2459 - 2464. [Abstract] [Full Text] [PDF]

				S. GHOSH Cholesteryl ester hydrolase in human monocyte/macrophage: cloning, sequencing, and expression of full-length cDNA Physiol Genomics, January 24, 2000; 2(1): 1 - 8. [Abstract] [Full Text] [PDF]

				I. Ishii, N. Yokoyama, M. Yanagimachi, N. Ashikawa, M. Hata, S. Murakami, Y. Asami, N. Morisaki, Y. Saito, S. Ohmori, et al. Stimulation of Cholesterol Release from Rabbit Foam Cells by the Action of a New Inhibitor for Acyl CoA:Cholesterol Acyltransferase (ACAT), HL-004 J. Pharmacol. Exp. Ther., October 1, 1998; 287(1): 115 - 121. [Abstract] [Full Text]

				J. S. Munday, K. G. Thompson, K. A. C. James, and B. W. Manktelow Dietary Antioxidants Do Not Reduce Fatty Streak Formation in the C57BL/6 Mouse Atherosclerosis Model Arterioscler Thromb Vasc Biol, January 1, 1998; 18(1): 114 - 119. [Abstract] [Full Text] [PDF]

				A. J. Brown, G. F. Watts, J. R. Burnett, R. T. Dean, and W. Jessup Sterol 27-Hydroxylase Acts on 7-Ketocholesterol in Human Atherosclerotic Lesions and Macrophages in Culture J. Biol. Chem., September 1, 2000; 275(36): 27627 - 27633. [Abstract] [Full Text] [PDF]