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(Arteriosclerosis, Thrombosis, and Vascular Biology. 1995;15:956-962.)
© 1995 American Heart Association, Inc.

Articles

Different Effects of Subclasses of HDL Containing ApoA-I but Not ApoA-II (LpA-I) on Cholesterol Esterification in Plasma and Net Cholesterol Efflux From Foam Cells

Takao Ohta; Keijiro Saku; Kouki Takata; Rie Nakamura; Yoichiro Ikeda; Ichiro Matsuda

From the Department of Pediatrics (T.O., R.N., Y.I., I.M.), Kumamoto University School of Medicine, Kumamoto, the Department of Internal Medicine (K.S.), Fukuoka University, Fukuoka, and the Hiroshima Railway Hospital (K.T.), Hiroshima, Japan.

Correspondence to Dr Takao Ohta, Department of Pediatrics, Kumamoto University School of Medicine, 1-1-1 Honjo, Kumamoto-City Kumamoto 860, Japan. E-mail ohta@gpo.kumamotou.ac.jp.

Abstract We investigated the effects of subclasses of plasma LpA-I (HDL containing apoA-I but not apoA-II) on cholesterol esterification in plasma and net cholesterol efflux from foam cells. LpA-I was composed of particles of three diameters: large (11.1 nm; Lg–LpA-I), medium (8.8 nm; Md–LpA-I), and small (7.7 nm; Sm–LpA-I). Plasma concentrations of LpA-I were positively correlated only with the level of Lg–LpA-I. Plasma concentrations of Lg–LpA-I were inversely correlated with the rate of cholesterol esterification in plasma and VLDL- and LDL-depleted plasma. Plasma concentrations of Md–LpA-I and Sm–LpA-I did not correlate with the rate of cholesterol esterification in plasma or VLDL- and LDL-depleted plasma. When macrophage foam cells were incubated with Md– and Sm–LpA-I, cellular cholesterol mass was reduced by approximately 70%. In contrast, the cellular cholesterol–reducing capacity of Lg–LpA-I was negligible. Lg–LpA-I inhibited net cholesterol removal from foam cells that was mediated by Md– and Sm–LpA-I and cholesteryl ester production with these particles. These results suggest that Md– and Sm–LpA-I may actively participate in cellular cholesterol removal and cholesterol esterification in plasma and HDL, while Lg–LpA-I may regulate these functions of Md– and Sm–LpA-I.

Key Words: apoA-I–containing lipoproteins • LCAT • LpA-I • LpA-I/A-II • reverse cholesterol transport

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