This Article Full Text Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Retzinger, G. S. Search for Related Content PubMed PubMed Citation Articles by Retzinger, G. S.

(Arteriosclerosis, Thrombosis, and Vascular Biology. 1995;15:786-792.)
© 1995 American Heart Association, Inc.

Articles

Adsorption and Coagulability of Fibrinogen on Atheromatous Lipid Surfaces

Gregory S. Retzinger

From the Department of Pathology and Laboratory Medicine, University of Cincinnati Medical Center, Cincinnati, Ohio.

Correspondence to Gregory S. Retzinger, MD, PhD, Department of Pathology and Laboratory Medicine, University of Cincinnati Medical Center, 231 Bethesda Ave, Cincinnati, OH 45267-0529.

Abstract Fibrinogen, the precursor of the blood clot matrix and a major constituent of atherosclerotic lesions, is shown to adsorb with high affinity to hydrophobic beads coated with cholesteryl oleate, cholesterol, or loosely packed lecithin. The quantity of fibrinogen that binds to cholesterol- or lecithin-coated beads decreases as the surface concentration of the lipid increases; densely packed films of lecithin bind little, if any, of the protein. In sharp contrast, the appreciable quantity of fibrinogen that binds to cholesteryl oleate–coated beads is indifferent to the surface concentration of that lipid. Not unexpectedly, the quantity of fibrinogen that binds to beads coated with mixtures of cholesteryl oleate and lecithin increases with increasing concentration of the cholesteryl ester. When bound, fibrinogen can be converted by thrombin to fibrin and nucleate clot formation as manifested by the aggregation of stirred beads. These results indicate that hydrophobic, atheromatous lipid surfaces, particularly those rich in cholesteryl esters, may be predisposed to thrombosis by virtue of their inherent capacity to bind functional fibrinogen.

Key Words: fibrinogen • lipid surfaces • atherosclerosis • thrombosis

This article has been cited by other articles:

				B. Varadi, K. Kolev, K. Tenekedjiev, G. Meszaros, I. Kovalszky, C. Longstaff, and R. Machovich Phospholipid Barrier to Fibrinolysis: ROLE FOR THE ANIONIC POLAR HEAD CHARGE AND THE GEL PHASE CRYSTALLINE STRUCTURE J. Biol. Chem., September 17, 2004; 279(38): 39863 - 39871. [Abstract] [Full Text] [PDF]

				A. S. Jakate, C. M. Einhaus, A. P. DeAnglis, G. S. Retzinger, and P. B. Desai Preparation, Characterization, and Preliminary Application of Fibrinogen-Coated Olive Oil Droplets for the Targeted Delivery of Docetaxel to Solid Malignancies Cancer Res., November 1, 2003; 63(21): 7314 - 7320. [Abstract] [Full Text] [PDF]

				G. S. Retzinger, A. P. DeAnglis, and S. J. Patuto Adsorption of Fibrinogen to Droplets of Liquid Hydrophobic Phases : Functionality of the Bound Protein and Biological Implications Arterioscler Thromb Vasc Biol, December 1, 1998; 18(12): 1948 - 1957. [Abstract] [Full Text] [PDF]