© 1995 American Heart Association, Inc.
Articles |
Selective Increase in Cholesterol at Atherosclerosis-Susceptible Aortic Sites After Short-term Cholesterol Feeding
From the Department of Pathology, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, NC.
Correspondence to Dawn C. Schwenke, PhD, Department of Pathology, Bowman Gray School of Medicine, Medical Center Blvd, Winston-Salem, NC 27157-1072. E-mail dschwenke@cpm.bgsm.wfu.edu.
Abstract In rabbits, the aortic arch and branch sites of the
descending thoracic and abdominal aortas are susceptible to
atherosclerosis. This study investigated the hypothesis
that the reported focal increase in LDL concentration and mean
residence time at susceptible aortic sites after feeding
cholesterol for 4 to 8 days precede atherosclerotic change
as indicated by increased aortic cholesterol concentration.
Cholesterol concentrations for all aortic sites of normal
rabbits were similar (
2.8 µmol/g). No change in aortic
cholesterol concentration could be detected after feeding
cholesterol for 8 days. However, after feeding
cholesterol for 12 and 16 days, cholesterol
concentrations for abdominal branch sites were increased compared with
abdominal branch sites of normal rabbits (4.47±0.50, n=8, and
4.85±0.33, n=11, µmol/g, respectively, versus 2.87±0.27, n=12,
µmol/g; P<.025 and P<.005, respectively). In
contrast, the cholesterol concentration of
atherosclerosis-resistant nonbranch
abdominal aorta was unchanged after feeding cholesterol for
16 days and was much less than that of the branch sites (2.72±0.12
versus 4.85±0.33 µmol/g, n=11; P<.001).
Cholesterol concentrations for other susceptible sites were
also increased after feeding cholesterol for 12 and 16
days. Cholesterol concentrations for susceptible sites were
linearly related to a combined measure of duration and extent of
hypercholesterolemia (P<.001 to
P<.0001), whereas no such relationship could be detected
for resistant sites. Most (59% to 93%) of the
cholesterol accumulating in susceptible aortic sites after
feeding cholesterol for 12 and 16 days was nonesterified,
suggesting that the increased cholesterol concentration did
not reflect development of foam cells or the insudation of plasma
lipoproteins. This study suggests that the reported focal increases in
LDL concentration and mean residence time at susceptible aortic sites
during cholesterol feeding precede
atherosclerosis.
Key Words: atherosclerosis • cholesterol • aorta • susceptibility • rabbit
This article has been cited by other articles:
 |
|
 |
|
  J. Jongstra-Bilen, M. Haidari, S.-N. Zhu, M. Chen, D. Guha, and M. I. Cybulsky Low-grade chronic inflammation in regions of the normal mouse arterial intima predisposed to atherosclerosis J. Exp. Med., September 4, 2006; 203(9): 2073 - 2083. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. Ruberti, C. A. Curcio, C. L. Millican, B. P. M. Menco, J.-D. Huang, and M. Johnson Quick-Freeze/Deep-Etch Visualization of Age-Related Lipid Accumulation in Bruch's Membrane Invest. Ophthalmol. Vis. Sci., April 1, 2003; 44(4): 1753 - 1759. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. von der Thusen, T. J.C. van Berkel, and E. A.L. Biessen Induction of Rapid Atherogenesis by Perivascular Carotid Collar Placement in Apolipoprotein E-Deficient and Low-Density Lipoprotein Receptor-Deficient Mice Circulation, February 27, 2001; 103(8): 1164 - 1170. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Schwenke Metabolic evidence for sequestration of low-density lipoprotein in abdominal aorta of normal rabbits Am J Physiol Heart Circ Physiol, September 1, 2000; 279(3): H1128 - H1140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Hajra, A. I. Evans, M. Chen, S. J. Hyduk, T. Collins, and M. I. Cybulsky The NF-kappa B signal transduction pathway in aortic endothelial cells is primed for activation in regions predisposed to atherosclerotic lesion formation PNAS, August 1, 2000; 97(16): 9052 - 9057. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. A. Truskey, R. A. Herrmann, J. Kait, and K. M. Barber Focal Increases in Vascular Cell Adhesion Molecule-1 and Intimal Macrophages at Atherosclerosis-Susceptible Sites in the Rabbit Aorta After Short-Term Cholesterol Feeding Arterioscler Thromb Vasc Biol, February 1, 1999; 19(2): 393 - 401. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Schwenke and S. R. Behr Vitamin E Combined With Selenium Inhibits Atherosclerosis in Hypercholesterolemic Rabbits Independently of Effects on Plasma Cholesterol Concentrations Circ. Res., August 24, 1998; 83(4): 366 - 377. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. C. Schwenke Gender Differences in Intima-Media Permeability to Low-Density Lipoprotein at Atherosclerosis-Prone Aortic Sites in Rabbits : Lack of Effect of 17ß-Estradiol Arterioscler Thromb Vasc Biol, October 1, 1997; 17(10): 2150 - 2157. [Abstract] [Full Text]
|
|
|
|
|
|
D. C. Schwenke Comparison of Aorta and Pulmonary Artery : I. Early Cholesterol Accumulation and Relative Susceptibility to Atheromatous Lesions Circ. Res., September 19, 1997; 81(3): 338 - 345. [Abstract] [Full Text]
|
|
|
|
|
|
D. C. Schwenke Comparison of Aorta and Pulmonary Artery : II. LDL Transport and Metabolism Correlate With Susceptibility to Atherosclerosis Circ. Res., September 19, 1997; 81(3): 346 - 354. [Abstract] [Full Text]
|
|
|
|
|
|
E. Hurt-Camejo, U. Olsson, O. Wiklund, G. Bondjers, and G. Camejo Cellular Consequences of the Association of ApoB Lipoproteins With Proteoglycans: Potential Contribution to Atherogenesis Arterioscler Thromb Vasc Biol, June 1, 1997; 17(6): 1011 - 1017. [Abstract] [Full Text]
|
|
|
|
|
|
E. Hurt-Camejo, S. Andersen, R. Standal, B. Rosengren, P. Sartipy, E. Stadberg, and B. Johansen Localization of Nonpancreatic Secretory Phospholipase A2 in Normal and Atherosclerotic Arteries: Activity of the Isolated Enzyme on Low-Density Lipoproteins Arterioscler Thromb Vasc Biol, February 1, 1997; 17(2): 300 - 309. [Abstract] [Full Text]
|
|