Arteriosclerosis and Thrombosis, Vol 14, 115-124, Copyright © 1994 by American Heart Association

ARTICLES

Glycosaminoglycan fractions from human arteries presenting diverse susceptibilities to atherosclerosis have different binding affinities to plasma LDL

LE Cardoso and PA Mourao
Departamento de Bioquimica, Universidade Federal do Rio de Janeiro, Brazil.

The topographic distribution of atherosclerotic lesions is influenced by biochemical factors intrinsic to the arterial wall. In the present work we have investigated whether the composition/chemical structure of glycosaminoglycans constitutes one of these factors. Normal human arteries were obtained at necropsy, and in order of decreasing susceptibility to atherosclerosis, consisted of the abdominal and thoracic aortas and the iliac and pulmonary arteries. The results showed similar concentrations of total glycosaminoglycan and collagen. Of the glycosaminoglycans known to interact with low-density lipoprotein (LDL), dermatan sulfate was present in all arteries in comparable concentrations, but the aortas had a 30% higher content of chondroitin 4/6-sulfate, which in turn was slightly enriched in 6- sulfated disaccharide units. LDL-affinity chromatography with dermatan sulfate+chondroitin 4/6-sulfate fractions demonstrated that increasing affinity to LDL matched an increasing susceptibility to atherosclerosis. Analysis of glycosaminoglycans in the eluates indicated a positive correlation between affinity to LDL and increasing molecular weight and the existence of a fraction of glycosaminoglycans of high affinity to LDL in the aortas only. These results suggest that arterial glycosaminoglycans participate in the multifactorial mechanisms that modulate the differential localization of atherosclerotic lesions.

This article has been cited by other articles:

				I. Tabas, K. J. Williams, and J. Boren Subendothelial Lipoprotein Retention as the Initiating Process in Atherosclerosis: Update and Therapeutic Implications Circulation, October 16, 2007; 116(16): 1832 - 1844. [Abstract] [Full Text] [PDF]

				M. Y. Chang, C.-Y. Han, T. N. Wight, and A. Chait Antioxidants Inhibit the Ability of Lysophosphatidylcholine to Regulate Proteoglycan Synthesis Arterioscler. Thromb. Vasc. Biol., March 1, 2006; 26(3): 494 - 500. [Abstract] [Full Text] [PDF]

				M. Rodriguez-Lee, G. Ostergren-Lunden, B. Wallin, J. Moses, G. Bondjers, and G. Camejo Fatty Acids Cause Alterations of Human Arterial Smooth Muscle Cell Proteoglycans That Increase the Affinity for Low-Density Lipoprotein Arterioscler. Thromb. Vasc. Biol., January 1, 2006; 26(1): 130 - 135. [Abstract] [Full Text] [PDF]

				D. O. Pinto, P. L. Ferreira, L. R. Andrade, H. Petrs-Silva, R. Linden, E. Abdelhay, H. M. M. Araujo, C.-E. V. Alonso, and M. S.G. Pavao Biosynthesis and metabolism of sulfated glycosaminoglycans during Drosophila melanogaster development Glycobiology, June 1, 2004; 14(6): 529 - 536. [Abstract] [Full Text] [PDF]

				T. N. Wight and M. J. Merrilees Proteoglycans in Atherosclerosis and Restenosis: Key Roles for Versican Circ. Res., May 14, 2004; 94(9): 1158 - 1167. [Abstract] [Full Text] [PDF]

				M. Y. Chang, C. Tsoi, T. N. Wight, and A. Chait Lysophosphatidylcholine Regulates Synthesis of Biglycan and the Proteoglycan Form of Macrophage Colony Stimulating Factor Arterioscler. Thromb. Vasc. Biol., May 1, 2003; 23(5): 809 - 815. [Abstract] [Full Text] [PDF]

				P. J. Little, L. Tannock, K. L. Olin, A. Chait, and T. N. Wight Proteoglycans Synthesized by Arterial Smooth Muscle Cells in the Presence of Transforming Growth Factor-{beta}1 Exhibit Increased Binding to LDLs Arterioscler. Thromb. Vasc. Biol., January 1, 2002; 22(1): 55 - 60. [Abstract] [Full Text] [PDF]

				A.-C. E.S. Vilela-Silva, C. C. Werneck, A. P. Valente, V. D. Vacquier, and P. A.S. Mourao Embryos of the sea urchin Strongylocentrotus purpuratus synthesize a dermatan sulfate enriched in 4-O- and 6-O-disulfated galactosamine units Glycobiology, June 1, 2001; 11(6): 433 - 440. [Abstract] [Full Text] [PDF]

				M. Gandra, M. C.M. Cavalcante, and M. S.G. Pavao Anticoagulant sulfated glycosaminoglycans in the tissues of the primitive chordate Styela plicata (Tunicata) Glycobiology, December 1, 2000; 10(12): 1333 - 1340. [Abstract] [Full Text] [PDF]

				D. C. Schwenke Metabolic evidence for sequestration of low-density lipoprotein in abdominal aorta of normal rabbits Am J Physiol Heart Circ Physiol, September 1, 2000; 279(3): H1128 - H1140. [Abstract] [Full Text] [PDF]

				M. Y. Chang, S. Potter-Perigo, C. Tsoi, A. Chait, and T. N. Wight Oxidized Low Density Lipoproteins Regulate Synthesis of Monkey Aortic Smooth Muscle Cell Proteoglycans That Have Enhanced Native Low Density Lipoprotein Binding Properties J. Biol. Chem., February 18, 2000; 275(7): 4766 - 4773. [Abstract] [Full Text] [PDF]

				U. Lundstam, E. Hurt-Camejo, G. Olsson, P. Sartipy, G. Camejo, and O. Wiklund Proteoglycans Contribution to Association of Lp(a) and LDL With Smooth Muscle Cell Extracellular Matrix Arterioscler. Thromb. Vasc. Biol., May 1, 1999; 19(5): 1162 - 1167. [Abstract] [Full Text] [PDF]

				M. S. G. Pavao, K. R. M. Aiello, C. C. Werneck, L. C. F. Silva, A.-P. Valente, B. Mulloy, N. S. Colwell, D. M. Tollefsen, and P. A. S. Mourao Highly Sulfated Dermatan Sulfates from Ascidians. STRUCTURE VERSUS ANTICOAGULANT ACTIVITY OF THESE GLYCOSAMINOGLYCANS J. Biol. Chem., October 23, 1998; 273(43): 27848 - 27857. [Abstract] [Full Text] [PDF]

				A. M. F. Tovar, D. C. F. Cesar, G. C. Leta, and P. A. S. Mourao Age-Related Changes in Populations of Aortic Glycosaminoglycans : Species With Low Affinity for Plasma Low-Density Lipoproteins, and Not Species With High Affinity, Are Preferentially Affected Arterioscler. Thromb. Vasc. Biol., April 1, 1998; 18(4): 604 - 614. [Abstract] [Full Text] [PDF]

				E. Hurt-Camejo, U. Olsson, O. Wiklund, G. Bondjers, and G. Camejo Cellular Consequences of the Association of ApoB Lipoproteins With Proteoglycans: Potential Contribution to Atherogenesis Arterioscler. Thromb. Vasc. Biol., June 1, 1997; 17(6): 1011 - 1017. [Abstract] [Full Text]

				P. Sartipy, B. Johansen, G. Camejo, B. Rosengren, G. Bondjers, and E. Hurt-Camejo Binding of Human Phospholipase A2 Type II to Proteoglycans. DIFFERENTIAL EFFECT OF GLYCOSAMINOGLYCANS ON ENZYME ACTIVITY J. Biol. Chem., October 18, 1996; 271(42): 26307 - 26314. [Abstract] [Full Text] [PDF]

				P. A.S. Mourao, M. S. Pereira, M. S.G. Pavao, B. Mulloy, D. M. Tollefsen, M.-C. Mowinckel, and U. Abildgaard Structure and Anticoagulant Activity of a Fucosylated Chondroitin Sulfate from Echinoderm. SULFATED FUCOSE BRANCHES ON THE POLYSACCHARIDE ACCOUNT FOR ITS HIGH ANTICOAGULANT ACTION J. Biol. Chem., September 27, 1996; 271(39): 23973 - 23984. [Abstract] [Full Text] [PDF]

				K. J. Williams and I. Tabas The Response-to-Retention Hypothesis of Early Atherogenesis Arterioscler. Thromb. Vasc. Biol., May 1, 1995; 15(5): 551 - 561. [Full Text]