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Published Online
on September 14, 2006

Arteriosclerosis, Thrombosis, and Vascular Biology. 2006
Published online before print September 14, 2006, doi: 10.1161/01.ATV.0000245797.76062.2e
A more recent version of this article appeared on November 1, 2006
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Submitted on April 4, 2006
Accepted on August 22, 2006

Phospholipase A2-Modified Low-Density Lipoprotein Activates the Phosphatidylinositol 3-Kinase-Akt Pathway and Increases Cell Survival in Monocytic Cells

Dmitry Namgaladze and Bernhard Brüne *

From Faculty of Medicine, Institute of Biochemistry I, Johann Wolfgang Goethe-University, Frankfurt, Germany.

* To whom correspondence should be addressed. E-mail: bruene{at}zbc.kgu.de.

Objective--Monocyte survival is an important determinant in the development of atherosclerotic lesions. We investigated the influence of phospholipase A2-modified LDL (PLA-LDL), a pro-atherogenic factor, on activation of the pro-survival kinase Akt and cell death in monocytic cells.

Methods and Results--PLA-LDL induced robust phosphorylation and activation of Akt in THP1 cells. It also attenuated oxidative stress-induced cell death, an effect abolished by phosphatidylinositol 3-kinase (PI3K) inhibition. In addition, PLA-LDL increased survival of human monocytes. We noticed that lipid products derived from LDL phospholipolysis are mediators of PLA-LDL-induced Akt activation. Arachidonic acid, which is released on phospholipase treatment of LDL, induced Akt phosphorylation and increased cell survival, whereas lysophosphatidylcholine, another compound generated by LDL phospholipolysis, induced only transient Akt phosphorylation and was cytotoxic.

Conclusions--Our data indicate that PLA-LDL induces activation of the PI3K-Akt pathway and promotes monocytic cell survival, which may contribute to the pro-atherogenic effects of phospholipase A2-modified LDL.
Key words: apoptosis • atherosclerosis • low-density lipoprotein • monocytes • phospholipase A2




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