on February 23, 2006
Published online before print February 23, 2006, doi: 10.1161/01.ATV.0000215001.92941.6c
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on August 18, 2005
Accepted on January 4, 2006
Intercellular Adhesion Molecule-1 Is Upregulated in Ischemic Muscle, Which Mediates Trafficking of Endothelial Progenitor Cells
Chang-Hwan Yoon ;
From the Cardiovascular Laboratory (C.-H.Y., J.H., I.O., K.-W.P., T.-Y.K., J.-H.K., C.-S.L., Y.-B.P., H.-S.K.), Clinical Research Institute, Seoul National University Hospital; Department of Nuclear Medicine (J.-H.S., J.-K.C.), Seoul National University College of Medicine; Department of Internal Medicine (Y.-B.P., H.-S.K.), Seoul National University College of Medicine, Seoul, Korea.
* To whom correspondence should be addressed. E-mail: hyosoo{at}snu.ac.kr.
Background--Trafficking of transplanted endothelial progenitor cells (EPCs) to an ischemic organ is a critical step in neovascularization. This study was performed to elucidate the molecular mechanism of EPC trafficking in terms of adhesion molecules.
Methods and Results--Using murine hindlimb ischemia model, we examined expressions of E-selectin, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and PECAM-1 in ischemic muscle by immunofluorescence. ICAM-1 was overexpressed in ischemic muscle compared with nonischemic muscle, whereas expressions of E-selectin, VCAM-1, and PECAM-1 did not show that much difference. ICAM-1 was also upregulated by hypoxia in murine endothelial cells (ECs) as assessed by immunoblot and flow cytometry. EPCs were attached to ECs specifically through ICAM-1/
-2 integrin interaction in vitro. When EPCs were labeled with fluorescent dye or radioisotope (Tc-99m-HMPAO) and systemically administrated in vivo, EPCs preferentially homed to ischemic muscle. By blocking ICAM-1, EPCs entrapment to ischemic limb in vivo was significantly reduced and neovascularization induced by EPC transplantation was attenuated.
Conclusions--ICAM-1 is upregulated by ischemia, and this is closely associated with EPCs entrapment to ischemic limb. Our findings suggest that ICAM-1 expression might be important in regulating the process of neovascularization through its ability to recruit EPCs.
Key words: angiogenesis • adhesion molecules • endothelium • endothelial progenitor cells • ischemia
This article has been cited by other articles:
 |
|
 |
|
  A. D. Bhatwadekar, J. V. Glenn, T. M. Curtis, M. B. Grant, A. W. Stitt, and T. A. Gardiner Retinal Endothelial Cell Apoptosis Stimulates Recruitment of Endothelial Progenitor Cells Invest. Ophthalmol. Vis. Sci., October 1, 2009; 50(10): 4967 - 4973. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. P Sieveking and M. K. Ng Cell therapies for therapeutic angiogenesis: back to the bench Vascular Medicine, May 1, 2009; 14(2): 153 - 166. [Abstract] [PDF]
|
|
|
|
 |
|
 K. K. Hirschi, D. A. Ingram, and M. C. Yoder Assessing Identity, Phenotype, and Fate of Endothelial Progenitor Cells Arterioscler Thromb Vasc Biol, September 1, 2008; 28(9): 1584 - 1595. [Full Text] [PDF]
|
|
|
|
 |
|
 C. Z. Behm, B. A. Kaufmann, C. Carr, M. Lankford, J. M. Sanders, C. E. Rose, S. Kaul, and J. R. Lindner Molecular Imaging of Endothelial Vascular Cell Adhesion Molecule-1 Expression and Inflammatory Cell Recruitment During Vasculogenesis and Ischemia-Mediated Arteriogenesis Circulation, June 3, 2008; 117(22): 2902 - 2911. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Zampetaki, J. P. Kirton, and Q. Xu Vascular repair by endothelial progenitor cells Cardiovasc Res, June 1, 2008; 78(3): 413 - 421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I.-Y. Oh, C.-H. Yoon, J. Hur, J.-H. Kim, T.-Y. Kim, C.-S. Lee, K.-W. Park, I.-H. Chae, B.-H. Oh, Y.-B. Park, et al. Involvement of E-selectin in recruitment of endothelial progenitor cells and angiogenesis in ischemic muscle Blood, December 1, 2007; 110(12): 3891 - 3899. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. R. Stenmark, K. A. Fagan, and M. G. Frid Hypoxia-Induced Pulmonary Vascular Remodeling: Cellular and Molecular Mechanisms Circ. Res., September 29, 2006; 99(7): 675 - 691. [Abstract] [Full Text] [PDF]
|
|