on November 23, 2005
Published online before print November 23, 2005, doi: 10.1161/01.ATV.0000197793.83391.91
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on August 2, 2005
Accepted on November 2, 2005
Isolation of "Side Population" Progenitor Cells From Healthy Arteries of Adult Mice
Julie Sainz *;
From INSERM E-0016, Faculté de Médecine Paris V, Université René Descartes, Paris, France.
* To whom correspondence should be addressed. E-mail: sainz{at}necker.fr.
Objective--Circulating progenitors and stem cells have been reported to contribute to angiogenesis and arterial repair after injury. In the present study, we investigated whether the arterial wall could host permanently residing progenitor cells under physiological context.
Methods and Results--Using the Hoechst-based flow cytometry method, we identified and isolated progenitor cells termed side population (SP) cells at a prevalence of 6.0±0.8% in the tunica media of adult mice aortas. Arterial SP cells expressed the ATP-binding cassette transporter subfamily G member 2, frequently present on SP cell surface, and displayed a Sca-1+ c-kit-/low Lin- CD34-/low profile. They did not form myeloid or lymphoid hematopoietic colonies after plating in methylcellulose-based medium. Importantly, cultured SP cells were able to acquire the phenotype of endothelial cells (CD31, VE-cadherin, and von Willebrand factor expression) or of smooth muscle cells (
-smooth muscle actin, calponin, and smooth muscle myosin heavy chain expression), in presence of either VEGF or transforming growth factor (TGF)-
1/PDFG-BB, respectively. Moreover, they generated vascular-like branching structures, composed of both VE-cadherin+ cells and -smooth muscle actin+ cells on Matrigel.
Conclusions--In this study, we provide the first evidence to our knowledge that in the adult mice, the normal arterial wall harbors SP cells with vascular progenitor properties.
Key words: endothelial cells • smooth muscle cells • SP cells • vascular progenitors • vasculogenesis
This article has been cited by other articles:
 |
|
 |
|
  Q. Liu, Z. Chen, T. Terry, J. M. McNatt, J. T. Willerson, and P. Zoldhelyi Intra-Arterial Transplantation of Adult Bone Marrow Cells Restores Blood Flow and Regenerates Skeletal Muscle in Ischemic Limbs Vascular and Endovascular Surgery, October 1, 2009; 43(5): 433 - 443. [Abstract] [PDF]
|
|
|
|
 |
|
 L. Rodriguez-Menocal, M. St-Pierre, Y. Wei, S. Khan, D. Mateu, M. Calfa, A. A. Rahnemai-Azar, G. Striker, S. M. Pham, and R. I. Vazquez-Padron The origin of post-injury neointimal cells in the rat balloon injury model Cardiovasc Res, January 1, 2009; 81(1): 46 - 53. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. N. Milovanova, V. M. Bhopale, E. M. Sorokina, J. S. Moore, T. K. Hunt, M. Hauer-Jensen, O. C. Velazquez, and S. R. Thom Lactate Stimulates Vasculogenic Stem Cells via the Thioredoxin System and Engages an Autocrine Activation Loop Involving Hypoxia-Inducible Factor 1 Mol. Cell. Biol., October 15, 2008; 28(20): 6248 - 6261. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Stevens, S. Phan, M. G. Frid, D. Alvarez, E. Herzog, and K. R. Stenmark Lung Vascular Cell Heterogeneity: Endothelium, Smooth Muscle, and Fibroblasts Proceedings of the ATS, September 15, 2008; 5(7): 783 - 791. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Xu Stem Cells and Transplant Arteriosclerosis Circ. Res., May 9, 2008; 102(9): 1011 - 1024. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Rienstra, C. J. Zeebregts, and J.-L. Hillebrands The Source of Neointimal Cells in Vein Grafts: Does the Origin Matter? Am. J. Pathol., March 1, 2008; 172(3): 566 - 570. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Maiellaro and W. R. Taylor The role of the adventitia in vascular inflammation Cardiovasc Res, September 1, 2007; 75(4): 640 - 648. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. W. Majesky Developmental Basis of Vascular Smooth Muscle Diversity Arterioscler Thromb Vasc Biol, June 1, 2007; 27(6): 1248 - 1258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Raisky, A. I. Nykanen, R. Krebs, M. Hollmen, M. A.I. Keranen, J. M. Tikkanen, R. Sihvola, L. Alhonen, P. Salven, Y. Wu, et al. VEGFR-1 and -2 Regulate Inflammation, Myocardial Angiogenesis, and Arteriosclerosis in Chronically Rejecting Cardiac Allografts Arterioscler Thromb Vasc Biol, April 1, 2007; 27(4): 819 - 825. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Xiao, L. Zeng, Z. Zhang, Y. Hu, and Q. Xu Stem cell-derived Sca-1+ progenitors differentiate into smooth muscle cells, which is mediated by collagen IV-integrin {alpha}1/beta1/{alpha}v and PDGF receptor pathways Am J Physiol Cell Physiol, January 1, 2007; 292(1): C342 - C352. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Paruchuri, J.-H. Yang, E. Aikawa, J. M. Melero-Martin, Z. A. Khan, S. Loukogeorgakis, F. J. Schoen, and J. Bischoff Human Pulmonary Valve Progenitor Cells Exhibit Endothelial/Mesenchymal Plasticity in Response to Vascular Endothelial Growth Factor-A and Transforming Growth Factor-{beta}2 Circ. Res., October 13, 2006; 99(8): 861 - 869. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Tso, G. Martinic, W.-H. Fan, C. Rogers, K.-A. Rye, and P. J. Barter High-Density Lipoproteins Enhance Progenitor-Mediated Endothelium Repair in Mice Arterioscler Thromb Vasc Biol, May 1, 2006; 26(5): 1144 - 1149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. P. Brandes Novel Faces to Old Friends: A Central Role of Inducible NO Synthase for Progenitor Cell Recruitment and a New Antiinflammatory Mechanisms of Statins Circ. Res., February 17, 2006; 98(3): 303 - 305. [Full Text] [PDF]
|
|