Donate Help Contact The AHA Sign In Home
American Heart Association
Arteriosclerosis, Thrombosis, and Vascular Biology
Search: search_blue_button Advanced Search
Published Online
on November 10, 2005

Arteriosclerosis, Thrombosis, and Vascular Biology. 2005
Published online before print November 10, 2005, doi: 10.1161/01.ATV.0000195791.83380.4c
A more recent version of this article appeared on February 1, 2006
This Article
Right arrow Full Text (PDF)
Right arrow Data Supplement
Right arrow All Versions of this Article:
26/2/365    most recent
01.ATV.0000195791.83380.4cv1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Teupser, D.
Right arrow Articles by Thiery, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Teupser, D.
Right arrow Articles by Thiery, J.
Right arrowPubmed/NCBI databases
*Substance via MeSH
Related Collections
Right arrowRelated Article

Submitted on June 8, 2005
Accepted on November 1, 2005

Identification of Macrophage Arginase I as a New Candidate Gene of Atherosclerosis Resistance

Daniel Teupser ; Ralph Burkhardt ; Wolfgang Wilfert ; Ivonne Haffner ; Klaus Nebendahl ; and Joachim Thiery *

From the Institute of Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics (D.T., R.B., W.W., I.H., J.T.), University Hospital Leipzig, Leipzig; and the Department of Experimental Animal Research (K.N.), University Hospital Goettingen, Germany.

* To whom correspondence should be addressed. E-mail: thiery{at}medizin.uni-leipzig.de.

Objective--Our laboratory has previously created 2 strains of rabbits with genetically determined high- and low- (LAR) atherosclerotic response. The aim of the present study was to identify new genes of atherosclerosis susceptibility in macrophages from the 2 strains.

Methods and Results--Suppression subtractive hybridization was used to screen for genes with higher expression in macrophages from LAR rabbits. We identified a cDNA fragment with high homology to human arginase I (AI; 91%) and subsequently cloned the full-length cDNA of the rabbit homologue. Quantitative RT-PCR revealed a significantly higher macrophage AI mRNA expression in LAR rabbits than in high-atherosclerotic response rabbits (77428±10941 versus 34344±4538; P=0.002; copies/106 copies {beta}-actin), which also correlated with a significantly higher arginase enzyme activity. Northern blot analysis led to the identification of a full-length polymorphism of AI mRNA. This was because of a 413 bp C-repeat insertion in the 3' untranslated region. The shorter transcript variant was predominantly expressed in LAR rabbits and associated with significantly higher AI mRNA expression levels. Transfection experiments indicated decreased mRNA stability of the long AI variant.

Conclusions--High expression of arginase I in macrophages may contribute to atherosclerosis resistance of LAR rabbits, possibly by conferring antiinflammatory effects in the vessel wall.
Key words: macrophages • gene expression • arginase I • animal models • atherosclerosis


Related Article:

Arginine/Arginase NO NO NO
Godfrey S. Getz and Catherine A. Reardon
Arterioscler Thromb Vasc Biol 2006 26: 237-239. [Extract] [Full Text] [PDF]



This article has been cited by other articles:


Home page
 J Am Coll CardiolHome page
R. I. Levin
Arginine and Old MACE Small Molecules in Atherogenesis Support the Concept of Coronary Artery Disease as a Complex Trait.
J. Am. Coll. Cardiol., June 2, 2009; 53(22): 2068 - 2069.
[Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
D. Teupser, S. Wolfrum, M. Tan, A. D. Persky, H. M. Dansky, and J. L. Breslow
Novel Strategy Using F1-Congenic Mice for Validation of QTLs: Studies at the Proximal Chromosome 10 Atherosclerosis Susceptibility Locus
Arterioscler Thromb Vasc Biol, May 1, 2009; 29(5): 678 - 683.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
A. Gallardo-Soler, C. Gomez-Nieto, M. L. Campo, C. Marathe, P. Tontonoz, A. Castrillo, and I. Corraliza
Arginase I Induction by Modified Lipoproteins in Macrophages: A Peroxisome Proliferator-Activated Receptor-{gamma}/{delta}-Mediated Effect that Links Lipid Metabolism and Immunity
Mol. Endocrinol., June 1, 2008; 22(6): 1394 - 1402.
[Abstract] [Full Text] [PDF]

Home page
 J. Med. Genet.Home page
J. Dumont, M. Zureik, D. Cottel, M. Montaye, P. Ducimetiere, P. Amouyel, and T. Brousseau
Association of arginase 1 gene polymorphisms with the risk of myocardial infarction and common carotid intima media thickness
J. Med. Genet., August 1, 2007; 44(8): 526 - 531.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
A. C. Thomas, G. B. Sala-Newby, Y. Ismail, J. L. Johnson, G. Pasterkamp, and A. C. Newby
Genomics of Foam Cells and Nonfoamy Macrophages From Rabbits Identifies Arginase-I as a Differential Regulator of Nitric Oxide Production
Arterioscler Thromb Vasc Biol, March 1, 2007; 27(3): 571 - 577.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
G. S. Getz and C. A. Reardon
Arginine/Arginase NO NO NO
Arterioscler Thromb Vasc Biol, February 1, 2006; 26(2): 237 - 239.
[Full Text] [PDF]


ATVB Home | Subscriptions | Archives | Feedback | Authors | Help | AHA Journals Home | Search
Copyright © 2005 American Heart Association, Inc. All rights reserved. Unauthorized use prohibited.