on January 13, 2005
Published online before print January 13, 2005, doi: 10.1161/01.ATV.0000155987.26583.9b
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Submitted on July 23, 2004
Accepted on January 5, 2005
Platelet Heterogeneity. Variation in Coagulation Complexes on Platelet Subpopulations
Christine L. Kempton ;
From the University of North Carolina, Chapel Hill, Department of Medicine, Division of Hematology-Oncology (C.L.K., H.R.R., D.M.M.); and Durham Veterans Affairs Medical Center (M.H.), Department of Pathology, Carolina Cardiovascular Biology Center, North Carolina.
* To whom correspondence should be addressed. E-mail: dmonroe{at}med.unc.edu.
Objective--Previous work has shown that platelets stimulated with the combination of thrombin and convulxin, a glycoprotein VI agonist, develop 2 populations with different levels of 
-granule factor V bound to the platelet surface. To evaluate whether this phenomenon is restricted to -granule components or is a feature that can be generalized to other coagulation factors, we studied the binding of factors V, VIII, IX, and X on the surface of platelets stimulated by convulxin and thrombin.
Methods and Results--The relative amount of factors V, VIII, IX, and X on the surface of platelets stimulated with thrombin or convulxin plus thrombin was measured using flow cytometry. Simultaneous measurements of factor Xa and thrombin generation were obtained and correlated with the binding of coagulation factors on the platelet surface. The binding of factors V, VIII, IX, and X all increased on a subpopulation of platelets when stimulated with the combined agonists. The development of this platelet subpopulation is dependent on convulxin concentration and correlates with increases in factor Xa and thrombin generation.
Conclusions--The development of increased coagulation factor binding to a subpopulation of platelets is not limited to -granule components. Convulxin-induced increases in thrombin generation are regulated by the proportion of platelets with increased coagulation factor binding.
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