on January 6, 2005
Published online before print January 6, 2005, doi: 10.1161/01.ATV.0000155017.60171.88
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Submitted on September 19, 2004
Accepted on December 12, 2004
Identification of Unique Lipoprotein Subclasses for Visceral Obesity by Component Analysis of Cholesterol Profile in High-Performance Liquid Chromatography
Mitsuyo Okazaki *;
From the Laboratory of Chemistry (M.O.), College of Liberal Arts and Sciences, Tokyo Medical and Dental University, Ichikawa; Faculty of Health Sciences (S.U.), Okayama University Medical School, Okayama; and the Department of Internal Medicine and Molecular Science (M.I., N.S., T.N., Y.M., S.Y.), Osaka University Graduate School of Medicine, Osaka, Japan.
* To whom correspondence should be addressed. E-mail: okazaki.las{at}tmd.ac.jp.
Objective--The contribution of visceral fat accumulation to the development of coronary heart disease was previously reported, but the relation between visceral fat accumulation and serum lipoprotein subclasses was unknown.
Methods and Results--We examined the relation of lipoprotein subclasses with visceral fat accumulation in 62 male subjects (aged 22 to 67 years) with visceral fat syndrome or obesity. Cholesterol levels in very low-density, low-density, and high-density lipoprotein subclasses (VLDL, LDL, and HDL) were determined by computer-assisted high-performance liquid chromatography. Subcutaneous fat area and visceral fat area were measured by computed tomographic scanning. There was no significant correlation between the subcutaneous fat area and the cholesterol levels in all lipoprotein subclasses. In contrast, the visceral fat area was correlated positively (P<0.002) with VLDL and LDL subclasses, except for large LDL, but negatively (P<0.001) with those in large and medium HDL subclasses. The observed positive correlations of small and very small LDL subclasses remained significant (P<0.005) after adjustment for serum cholesterol, triglycerides, HDL cholesterol, and LDL cholesterol, respectively, but a significant negative correlation (P<0.005) of large LDL was obtained after adjustment for LDL cholesterol.
Conclusion--These findings indicate that this simple high-performance liquid chromatography method may be applied for easy detection and evaluation of abnormal distribution of lipoprotein subclasses.
Key words: obesity • risk factors • particle size • triglyceride
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