on December 9, 2004
Published online before print December 9, 2004, doi: 10.1161/01.ATV.0000152726.49229.bf
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Submitted on September 27, 2004
Accepted on November 24, 2004
Role of Mitochondrial Permeability Transition Pore in Coated-Platelet Formation
Gyula Remenyi ;
From the W.K. Warren Medical Research Institute, Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City.
* To whom correspondence should be addressed. E-mail: george-dale{at}ouhsc.edu.
Objective--Coated platelets are a subset of cells observed during costimulation of platelets with collagen and thrombin. Important characteristics of coated platelets include retention of multiple 
-granule proteins and expression of phosphatidylserine on the cell surface. The mitochondrial permeability transition pore (MPTP) is a key step in apoptosis and is suggested to be involved in some forms of platelet activation. The objective of this study was to examine the role of MPTP in the synthesis of coated platelets.
Methods and Results--Flow cytometric analysis of coated-platelet production was used to examine the impact of pharmacological effectors of MPTP formation. Cyclosporin A, coenzyme Q, and bongkrekic acid all inhibit MPTP formation as well as production of coated platelets. Phenylarsine oxide and diamide, both potentiators of MPTP formation, stimulate coated-platelet synthesis. Atractyloside, another inducer of MPTP formation, does not affect the percentage of coated platelets synthesized; however, it does increase the level of phosphatidylserine exposed on the surface of coated platelets.
Conclusions--These findings indicate that MPTP formation is an integral event in the synthesis of coated platelets. Although the exact function of the MPTP remains to be determined, these data support a growing body of evidence that apoptosis-associated events are vital components of the platelet activation process.
Key words: coated platelet • mitochondrial permeability transition pore • coenzyme Q • phosphatidylserine • cyclosporin A • phenylarsine oxide • diamide
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