on November 4, 2004
Published online before print November 4, 2004, doi: 10.1161/01.ATV.0000149381.16166.c6
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on January 28, 2004
Accepted on September 30, 2004
Scavenger Receptor Class B Type I Mediates the Selective Uptake of High-Density Lipoprotein-Associated Cholesteryl Ester by the Liver in Mice
May Brundert ;
From University Hospital Hamburg-Eppendorf (M.B., A.E., J.H., B.B., H.G., M.M., F.R.), Hamburg, Germany; and the College of Physicians and Surgeons of Columbia University (R.R.), New York, NY.
* To whom correspondence should be addressed. E-mail: Rinninger{at}uke.uni-hamburg.de.
Objective--High-density lipoprotein (HDL) cholesteryl esters (CE) are taken up by liver and adrenals selectively, ie, independent from particle internalization. Class B type I scavenger receptor (SR-BI) mediates this uptake in vitro. The role of SR-BI in HDL metabolism was explored in mice.
Methods and Results--Mice with a mutation in the SR-BI gene (SR-BI KO) and wild-type (WT) littermates were used. Mutants had increased HDL cholesterol. HDL was labeled with 125I (protein) and [3H] (CE). After HDL injection, blood samples were drawn and finally the mice were euthanized. In WT, the plasma decay of HDL-associated [3H] is faster compared with 125I and this represents whole-body selective CE uptake. In SR-BI KO, the decay of both tracers is similar, yielding no selective CE removal. In WT liver and adrenals, uptake of [3H] is higher than 125I, showing selective uptake. In SR-BI KO, liver uptake of [3H] and 125I are similar, proposing no selective HDL CE uptake. In SR-BI KO adrenals, selective uptake is reduced; however, even in the absence of SR-BI, this uptake is detected using WT HDL.
Conclusions--SR-BI mediates selective uptake of HDL CE by the liver. In adrenals, an alternative mechanism or mechanisms can play a role in selective CE uptake.
This article has been cited by other articles:
 |
|
 |
|
  J. M. Karasinska, F. Rinninger, D. Lutjohann, P. Ruddle, S. Franciosi, J. K. Kruit, R. R. Singaraja, V. Hirsch-Reinshagen, J. Fan, L. R. Brunham, et al. Specific Loss of Brain ABCA1 Increases Brain Cholesterol Uptake and Influences Neuronal Structure and Function J. Neurosci., March 18, 2009; 29(11): 3579 - 3589. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Nijstad, H. Wiersma, T. Gautier, M. van der Giet, C. Maugeais, and U. J. F. Tietge Scavenger Receptor BI-mediated Selective Uptake Is Required for the Remodeling of High Density Lipoprotein by Endothelial Lipase J. Biol. Chem., March 6, 2009; 284(10): 6093 - 6100. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hoekstra, I. Meurs, M. Koenders, R. Out, R. B. Hildebrand, J. K. Kruijt, M. Van Eck, and T. J. C. Van Berkel Absence of HDL cholesteryl ester uptake in mice via SR-BI impairs an adequate adrenal glucocorticoid-mediated stress response to fasting J. Lipid Res., April 1, 2008; 49(4): 738 - 745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Dugue-Pujol, X. Rousset, D. Chateau, D. Pastier, C. Klein, J. Demeurie, C. Cywiner-Golenzer, M. Chabert, P. Verroust, J. Chambaz, et al. Apolipoprotein A-II is catabolized in the kidney as a function of its plasma concentration J. Lipid Res., October 1, 2007; 48(10): 2151 - 2161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Parathath, Y. F. Darlington, M. de la Llera Moya, D. Drazul-Schrader, D. L. Williams, M. C. Phillips, G. H. Rothblat, and M. A. Connelly Effects of amino acid substitutions at glycine 420 on SR-BI cholesterol transport function J. Lipid Res., June 1, 2007; 48(6): 1386 - 1395. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Harder, P. Lau, A. Meng, S. C. Whitman, and R. McPherson Cholesteryl Ester Transfer Protein (CETP) Expression Protects Against Diet Induced Atherosclerosis in SR-BI Deficient Mice Arterioscler Thromb Vasc Biol, April 1, 2007; 27(4): 858 - 864. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. C. Rideout, Z. Yuan, M. Bakovic, Q. Liu, R.-K. Li, Y. Mine, and M. Z. Fan Guar Gum Consumption Increases Hepatic Nuclear SREBP2 and LDL Receptor Expression in Pigs Fed an Atherogenic Diet J. Nutr., March 1, 2007; 137(3): 568 - 572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Tanaka, J. Delgado-Lista, J. Lopez-Miranda, F. Perez-Jimenez, C. Marin, P. Perez-Martinez, P. Gomez, and J. M. Ordovas Scavenger Receptor Class B Type I (SCARB1) c.1119C>T Polymorphism Affects Postprandial Triglyceride Metabolism in Men J. Nutr., March 1, 2007; 137(3): 578 - 582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Igarashi, J. C. DeMar Jr., K. Ma, L. Chang, J. M. Bell, and S. I. Rapoport Upregulated liver conversion of {alpha}-linolenic acid to docosahexaenoic acid in rats on a 15 week n-3 PUFA-deficient diet J. Lipid Res., January 1, 2007; 48(1): 152 - 164. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Brundert, J. Heeren, M. Bahar-Bayansar, A. Ewert, K. J. Moore, and F. Rinninger Selective uptake of HDL cholesteryl esters and cholesterol efflux from mouse peritoneal macrophages independent of SR-BI J. Lipid Res., November 1, 2006; 47(11): 2408 - 2421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Ikonen Mechanisms for cellular cholesterol transport: defects and human disease. Physiol Rev, October 1, 2006; 86(4): 1237 - 1261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Igarashi, K. Ma, L. Chang, J. M. Bell, S. I. Rapoport, and J. C. DeMar Jr. Low liver conversion rate of {alpha}-linolenic to docosahexaenoic acid in awake rats on a high-docosahexaenoate-containing diet J. Lipid Res., August 1, 2006; 47(8): 1812 - 1822. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. R. Singaraja, B. Stahmer, M. Brundert, M. Merkel, J. Heeren, N. Bissada, M. Kang, J. M. Timmins, R. Ramakrishnan, J. S. Parks, et al. Hepatic ATP-Binding Cassette Transporter A1 Is a Key Molecule in High-Density Lipoprotein Cholesteryl Ester Metabolism in Mice Arterioscler Thromb Vasc Biol, August 1, 2006; 26(8): 1821 - 1827. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Cuchel and D. J. Rader Macrophage Reverse Cholesterol Transport: Key to the Regression of Atherosclerosis? Circulation, May 30, 2006; 113(21): 2548 - 2555. [Full Text] [PDF]
|
|
|
|
|
|
A. Gauthier, P. Lau, X. Zha, R. Milne, and R. McPherson Cholesteryl Ester Transfer Protein Directly Mediates Selective Uptake of High Density Lipoprotein Cholesteryl Esters by the Liver Arterioscler Thromb Vasc Biol, October 1, 2005; 25(10): 2177 - 2184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Brodeur, V. Luangrath, G. Bourret, L. Falstrault, and L. Brissette Physiological importance of SR-BI in the in vivo metabolism of human HDL and LDL in male and female mice J. Lipid Res., April 1, 2005; 46(4): 687 - 696. [Abstract] [Full Text] [PDF]
|
|