on February 26, 2004
Published online before print February 26, 2004, doi: 10.1161/01.ATV.0000124103.86943.1e
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Submitted on December 24, 2003
Accepted on February 11, 2004
Rapid Perfusion and Network Remodeling in a Microvascular Construct After Implantation
Benjamin R. Shepherd ;
From the Biomedical Engineering Program (H.Y.S.C., C.M.S., S.K.W., J.B.H.) and Physiological Sciences Program (B.R.S., S.K.W., J.B.H.), Vascular Research Group, University of Arizona, Tucson. B.R.S. is currently at the Boyer Center for Molecular Medicine, Yale School of Medicine, New Haven, Conn.
* To whom correspondence should be addressed. E-mail: jhoying{at}u.arizona.edu.
Objective--We have previously demonstrated the ability to construct 3-dimensional microvascular beds in vitro via angiogenesis from isolated, intact, microvessel fragments that retain endothelial cells and perivascular cells. Our objective was to develop and characterize an experimental model of tissue vascularization, based on the implantation of this microvascular construct, which recapitulated angiogenesis, vessel differentiation, and network maturation.
Methods and Results--On implantation in a severe combined-immunodeficient mouse model, vessels in the microvascular constructs rapidly inosculated with the recipient host circulation. Ink perfusion of implants via the left ventricle of the host demonstrated that vessel inosculation begins within the first day after implantation. Evaluation of explanted constructs over the course of 28 days revealed the presence of a mature functional microvascular bed. Using a probe specific for the original microvessel source, 91.7%±11% and 88.6%±19% of the vessels by day 5 and day 28 after implantation, respectively, were derived from the original microvessel isolate. Similar results were obtained when human-derived microvessels were used to build the microvascular construct.
Conclusions--With this model, we reproduce the important aspects of vascularization, angiogenesis, inosculation, and network remodeling. Furthermore, we demonstrate that the model accommodates human-derived vessel fragments, enabling the construction of human-mouse vascular chimeras.
Key words: vascularization • microcirculation • angiogenesis • human • vascular remodeling
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