on July 31, 2003
Published online before print July 31, 2003, doi: 10.1161/01.ATV.0000089329.09061.07
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Submitted on April 3, 2003
Accepted on June 23, 2003
Serum Chitotriosidase Activity Is Increased in Subjects With Atherosclerosis Disease
Marta Artieda *;
From Laboratorio de Investigación Molecular (M.A., A.C., C.G., F.C.), Servicio de Cardiología (J.M.C.), and Servicio de Medicina Interna (I.V., F.C.), Hospital Universitario Miguel Servet, Zaragoza; Departamento de Bioquímica y Biología Molecular y Celular (A.G., M.P.), Universidad de Zaragoza; Servicio de Neurología (I.J.), Hospital Clínico Universitario Lozano Blesa, Zaragoza; and Servicio de Bioquímica (J.P.), Hospital San Jorge, Huesca, Spain.
* To whom correspondence should be addressed. E-mail: martieda{at}hmservet.insalud.es.
Objective--This study was undertaken to analyze the relation between serum activity of chitotriosidase enzyme, a protein synthesized exclusively by activated macrophages, and atherosclerotic lesion extent in subjects with atherothrombotic stroke (ATS) and in subjects with ischemic heart disease (IHD).
Methods and Results--We assayed the serum chitotriosidase activity and a common chitotriosidase gene polymorphism that causes deficiency in chitotriosidase activity in 3 Spanish populations, ATS (n=153), IHD (n=124), and control (n=148) subjects. Statistical differences were found in serum chitotriosidase activity between ATS (88.1±4.6 nmol/mL · h, P<0.0001) and IHD subjects (79.0±6.3, P=0.002) versus control group (70.9±5.2). These observed differences were not attributable to a distinct allelic or genotype distribution. The extension of the atherosclerotic lesion in carotids of ATS subjects was measured by duplex sonography. Chitotriosidase activities were 66.9±9.6, 88.7±8.3, and 107.7±11.8 for subjects with carotid stenosis 
30%, 31% to 60%, and >60%, respectively. Statistical differences were observed between subjects with major and intermediate stenosis grade compared with subjects with minor stenosis, P=0.005 and P=0.016, respectively.
Conclusions--Serum chitotriosidase activity is significantly increased in individuals suffering from atherosclerosis disease and is related to the severity of the atherosclerotic lesion, suggesting a possible role as atherosclerotic extent marker.
Key words: chitotriosidase • macrophage • atherothrombotic stroke • ischemic heart disease • atherosclerosis
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