on June 19, 2003
Arteriosclerosis, Thrombosis, and Vascular Biology. 2003
Published online before print June 19, 2003, doi: 10.1161/01.ATV.0000081743.35125.05
Published online before print June 19, 2003, doi: 10.1161/01.ATV.0000081743.35125.05
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Submitted on April 1, 2003
Accepted on May 21, 2003
Caveolin-1 Can Regulate Vascular Smooth Muscle Cell Fate by Switching Platelet-Derived Growth Factor Signaling From a Proliferative to an Apoptotic Pathway
Timothy E. Peterson ;
From the Division of Cardiovascular Diseases (T.E.P., R.G., L.S.K., C.S.M., M.M., R.D.S.), Department of Biochemistry and Molecular Biology and the Molecular Medicine Program, and the Division of Gastroenterology and Hepatology (M.E.G., G.J.G.), Mayo Clinic and Foundation, Rochester, Minn; and the Department of Pharmacology (G.S., W.C.S.), Yale University School of Medicine, Boyer Center for Molecular Medicine, New Haven, Conn.
* To whom correspondence should be addressed. E-mail: simari.robert{at}mayo.edu.
Background--Caveolin-1 is a regulator of signaling events originating from plasma membrane microdomains termed caveolae. This study was performed to determine the regulatory role of caveolin-1 on the proliferative events induced by platelet-derived growth factor (PDGF) in vascular smooth muscle cells (VSMCs).
Methods and Results--Treatment of VSMCs with PDGF for 24 hours resulted in a loss of caveolin-1 protein expression and plasma membrane-associated caveolae, despite a 3-fold increase in caveolin-1 mRNA. Pretreatment of VSMCs with chloroquine, an inhibitor of lysosomal function, inhibited the PDGF-induced loss of caveolin-1. These studies demonstrated that caveolin-1 was a target of PDGF signaling events. Adenoviral overexpression of caveolin-1 was associated with a switch in PDGF-induced signaling events from a proliferative response to an apoptotic response. This overexpression inhibited PDGF-induced expression of cyclin D1 in the presence of unaffected mitogen-activated protein kinase activation.
Conclusions--Taken together, these studies suggest that caveolin-1 is an inhibitor of PDGF proliferative responses and might be capable of transforming PDGF-induced proliferative signals into death signals.
Key words: caveolae • smooth muscle • proliferation • platelet-derived growth factor • apoptosis
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