on September 5, 2002
Published online before print September 5, 2002, doi: 10.1161/01.ATV.0000036418.04998.D5
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on August 7, 2002
Accepted on August 23, 2002
Endothelial Determinants of Dendritic Cell Adhesion and Migration. New Implications for Vascular Diseases
Michael Weis *;
From the Division of Cardiovascular Medicine (M.W., J.P.C.) and the Departments of Pathology and Medicine (C.L.S., E.G.E.), Stanford University School of Medicine, Stanford, Calif, and Medizinische Klinik I (M.W.), University Hospital Grosshadern, Ludwig-Maximilians-University of Munich, Munich, Germany.
* To whom correspondence should be addressed. E-mail: miweis{at}med.uni-muenchen.de.
Objective—Atherosclerosis is a chronic disease triggered by endothelial injury and sustained by inflammation. Dendritic cells (DCs) are critical for the cell-mediated arm of an immune response and are known to influence inflammatory immunity. A fundamental aspect of DC function is their capacity to adhere and migrate through vascular endothelial cells (ECs). We investigated the role of endothelial activation and dysregulation of the NO pathway on DC adhesion and migration.
Methods and Results—We discovered that DC adhesion and migration are modulated by changes in endothelial function. DC adhesion and transmigration were markedly increased after exposing ECs to hypoxia, oxidized low density lipoprotein, or tumor necrosis factor-
. Specifically, inhibition of endothelial NO synthase increased DC binding and transmigration. L-Arginine or 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibition partially decreased DC-EC interaction.
Conclusions—The results of this study suggest that the adhesion and migration of DCs are increased by stimuli known to accelerate atherogenesis. Vice versa, augmentation of endothelial NO synthase activity prevents DC adhesion. These findings may provide insight into the inflammatory processes occurring in atherosclerosis. Because DCs control immunity, regulating DC-EC interaction may be relevant to inflammation and atherogenesis.
Key words: atherosclerosis • 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors • inflammation • nitric oxide
This article has been cited by other articles:
 |
|
 |
|
  S. Negash, S. R. Narasimhan, W. Zhou, J. Liu, F. L. Wei, J. Tian, and J. U. Raj Role of cGMP-dependent protein kinase in regulation of pulmonary vascular smooth muscle cell adhesion and migration: effect of hypoxia Am J Physiol Heart Circ Physiol, July 1, 2009; 297(1): H304 - H312. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. R.S. Packard, E. Maganto-Garcia, I. Gotsman, I. Tabas, P. Libby, and A. H. Lichtman CD11c+ Dendritic Cells Maintain Antigen Processing, Presentation Capabilities, and CD4+ T-Cell Priming Efficacy Under Hypercholesterolemic Conditions Associated With Atherosclerosis Circ. Res., October 24, 2008; 103(9): 965 - 973. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. F. Bove, M. Hristova, U. V. Wesley, N. Olson, K. M. Lounsbury, and A. van der Vliet Inflammatory Levels of Nitric Oxide Inhibit Airway Epithelial Cell Migration by Inhibition of the Kinase ERK1/2 and Activation of Hypoxia-inducible Factor-1{alpha} J. Biol. Chem., June 27, 2008; 283(26): 17919 - 17928. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Wildenberg, C. G. van Helden-Meeuwsen, J. P. van de Merwe, C. Moreno, H. A. Drexhage, and M. A. Versnel Lack of CCR5 on dendritic cells promotes a proinflammatory environment in submandibular glands of the NOD mouse J. Leukoc. Biol., May 1, 2008; 83(5): 1194 - 1200. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Methe and M. Weis Atherogenesis and inflammation--was Virchow right? Nephrol. Dial. Transplant., July 1, 2007; 22(7): 1823 - 1827. [Full Text] [PDF]
|
|
|
|
 |
|
 R. Geyeregger, M. Zeyda, W. Bauer, E. Kriehuber, M. D. Saemann, G. J. Zlabinger, D. Maurer, and T. M. Stulnig Liver X receptors regulate dendritic cell phenotype and function through blocked induction of the actin-bundling protein fascin Blood, May 15, 2007; 109(10): 4288 - 4295. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S Ranjit and L Dazhu Potential role of dendritic cells for progression of atherosclerotic lesions. Postgrad. Med. J., September 1, 2006; 82(971): 573 - 575. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Yilmaz, J. Weber, I. Cicha, C. Stumpf, M. Klein, D. Raithel, W. G. Daniel, and C. D. Garlichs Decrease in Circulating Myeloid Dendritic Cell Precursors in Coronary Artery Disease J. Am. Coll. Cardiol., July 4, 2006; 48(1): 70 - 80. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Sharma and D.-Z. Li Role of Dendritic Cells in Atherosclerosis Asian Cardiovasc Thorac Ann, April 1, 2006; 14(2): 166 - 169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Yilmaz, C. Reiss, A. Weng, I. Cicha, C. Stumpf, A. Steinkasserer, W. G. Daniel, and C. D. Garlichs Differential effects of statins on relevant functions of human monocyte-derived dendritic cells J. Leukoc. Biol., March 1, 2006; 79(3): 529 - 538. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. I. van Leuven, J. J.P. Kastelein, A. C. Allison, M. R. Hayden, and E. S.G. Stroes Mycophenolate mofetil (MMF): Firing at the atherosclerotic plaque from different angles? Cardiovasc Res, February 1, 2006; 69(2): 341 - 347. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. De Palma, E. Meacci, C. Perrotta, P. Bruni, and E. Clementi Endothelial Nitric Oxide Synthase Activation by Tumor Necrosis Factor {alpha} Through Neutral Sphingomyelinase 2, Sphingosine Kinase 1, and Sphingosine 1 Phosphate Receptors: A Novel Pathway Relevant to the Pathophysiology of Endothelium Arterioscler Thromb Vasc Biol, January 1, 2006; 26(1): 99 - 105. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. V. Bobryshev Dendritic cells in atherosclerosis: current status of the problem and clinical relevance Eur. Heart J., September 1, 2005; 26(17): 1700 - 1704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. VanderLaan and C. A. Reardon Thematic review series: The Immune System and Atherogenesis. The unusual suspects:an overview of the minor leukocyte populations in atherosclerosis J. Lipid Res., May 1, 2005; 46(5): 829 - 838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Vassalli, A. Gallino, M. Weis, W. von Scheidt, L. Kappenberger, L.K. von Segesser, J.-J. Goy, and on behalf of the Working Group Microcirculation of Alloimmunity and nonimmunologic risk factors in cardiac allograft vasculopathy Eur. Heart J., July 1, 2003; 24(13): 1180 - 1188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Weis and J. P. Cooke Cardiac Allograft Vasculopathy and Dysregulation of the NO Synthase Pathway Arterioscler Thromb Vasc Biol, April 1, 2003; 23(4): 567 - 575. [Abstract] [Full Text] [PDF]
|
|