Donate Help Contact The AHA Sign In Home
American Heart Association
Arteriosclerosis, Thrombosis, and Vascular Biology
Search: search_blue_button Advanced Search
Published Online
on June 6, 2002

Arteriosclerosis, Thrombosis, and Vascular Biology. 2002
Published online before print June 6, 2002, doi: 10.1161/01.ATV.0000024684.67566.45
A more recent version of this article appeared on August 1, 2002
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
22/8/1286    most recent
01.ATV.0000024684.67566.45v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ichii, T.
Right arrow Articles by Nishizawa, Y.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Ichii, T.
Right arrow Articles by Nishizawa, Y.
Related Collections
Right arrow Other Ethics and Policy
Right arrow Glucose intolerance
Right arrow Coagulation and fibronolysis

Submitted on April 10, 2002
Accepted on May 10, 2002

Thrombospondin-1 Mediates Smooth Muscle Cell Proliferation Induced by Interaction With Human Platelets

Takuya Ichii ; Hidenori Koyama *; Shinji Tanaka ; Atsushi Shioi ; Yasuhisa Okuno ; Shuzo Otani ; and Yoshiki Nishizawa

From the Department of Biochemistry (T.I., S.O.), the Department of Metabolism, Endocrinology, and Molecular Medicine (H.K., S.T., Y.N.), and the Department of Cardiovascular Medicine (A.S., Y.O.), Osaka City University Graduate School of Medicine, Osaka, Japan.

* To whom correspondence should be addressed. E-mail: hidekoyama{at}med.osaka-cu.ac.jp.

Objectives—Platelet adherence and activation are associated with smooth muscle cell (SMC) proliferation and arterial restenosis. This study examined platelet-SMC interaction on fibrillar type I collagen and analyzed the role of thrombospondin (TSP)-1 in platelet-induced SMC proliferation.

Methods and Results—When SMCs cultured on fibrillar collagen were treated with human platelets (5 preparations), 7.45±2.94% of the cells passed through S phase within 24 hours, as determined by bromodeoxyuridine nuclear labeling. The addition of platelets markedly induced SMC TSP-1 mRNA expression and cell surface protein accumulation, which colocalized with adhered platelets, as determined by {alpha}IIb integrin immunostaining. Direct interaction of platelets with SMCs was necessary for its effect on proliferation and TSP-1 accumulation, as determined in the transwell culture system. The anti--TSP-1 blocking antibody strongly inhibited platelet-induced SMC proliferation by {approx}60%. Analysis of the receptors for TSP-1 accumulation on the SMC surface revealed that ß1 integrins are mainly involved. The anti--ß1 integrin blocking antibody, which potently suppressed TSP-1 accumulation on SMCs, also markedly inhibited platelet-stimulated SMC proliferation.

Conclusions—TSP-1 and ß1 integrin interaction is involved in platelet-stimulated SMC proliferation. This in vitro coculture system could prove useful for examining the molecular mechanism underlying platelet-induced vascular remodeling and for studying the mechanism of a tested drug for restenosis.
Key words: atherosclerosis • platelet-derived factors • integrins • collagen (type I) • vascular injury




This article has been cited by other articles:


Home page
 Molecular Cancer TherapeuticsHome page
M. K. McElroy, S. Kaushal, H. S. Tran Cao, A.R. Moossa, M. A. Talamini, R. M. Hoffman, and M. Bouvet
Upregulation of thrombospondin-1 and angiogenesis in an aggressive human pancreatic cancer cell line selected for high metastasis
Mol. Cancer Ther., July 1, 2009; 8(7): 1779 - 1786.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. C. Pitchford, Y. Riffo-Vasquez, A. Sousa, S. Momi, P. Gresele, D. Spina, and C. P. Page
Platelets are necessary for airway wall remodeling in a murine model of chronic allergic inflammation
Blood, January 15, 2004; 103(2): 639 - 647.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
H. Koyama, T. Maeno, S. Fukumoto, T. Shoji, T. Yamane, H. Yokoyama, M. Emoto, T. Shoji, H. Tahara, M. Inaba, et al.
Platelet P-Selectin Expression Is Associated With Atherosclerotic Wall Thickness in Carotid Artery in Humans
Circulation, August 5, 2003; 108(5): 524 - 529.
[Abstract] [Full Text] [PDF]


ATVB Home | Subscriptions | Archives | Feedback | Authors | Help | AHA Journals Home | Search
Copyright © 2002 American Heart Association, Inc. All rights reserved. Unauthorized use prohibited.