on May 23, 2002
Published online before print May 23, 2002, doi: 10.1161/01.ATV.0000022849.26083.FA
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on April 7, 2002
Accepted on April 28, 2002
Transvascular Low-Density Lipoprotein Transport in Patients With Diabetes Mellitus (Type 2). A Noninvasive In Vivo Isotope Technique
Karen Kornerup ;
From the Department of Nephrology and Endocrinology P (K.K., B.F.-R., K.S.J., J.S.J.), the National University Hospital; the "Copenhagen City Heart Study" (B.G.N., J.S.J.), Bispebjerg University Hospital; the Department of Clinical Biochemistry (B.G.N.), Herlev University Hospital; and Steno Diabetes Center (K.B.-J.), Copenhagen, Denmark.
* To whom correspondence should be addressed. E-mail: jsje{at}dadlnet.dk.
Objective—The increased risk of atherosclerosis associated with diabetes cannot be explained by conventional cardiovascular risk factors alone. We hypothesized that transvascular lipoprotein transport may be increased in patients with diabetes, possibly explaining increased intimal lipoprotein accumulation and, thus, atherosclerosis.
Methods and Results—We developed an in vivo method for measurement of transvascular transport of low density lipoprotein (LDL) and applied it in 16 patients with maturity-onset diabetes (type 2) and 29 healthy control subjects. Autologous 131I-labeled LDL was reinjected intravenously in addition to 125I-labeled albumin, and the 1-hour fractional escape rates were taken as indices of transvascular transport. Both parameters were normally distributed, and they were tightly correlated (R2=0.69, P<0.0001). Transvascular LDL transport was 5.4±2.9%/h and 4.1±1.5%/h in patients with diabetes and control subjects, respectively (P<0.05); equivalent values for albumin were 6.5±2.5%/h and 5.3±1.6%/h (P<0.05). This difference most likely was not caused by altered hepatic LDL receptor expression, glycosylation of LDL, small LDL size, nephropathy, statin use, or different plasma insulin levels in diabetic patients.
Conclusions—Transvascular LDL transport may be increased in patients with type 2 diabetes. This suggests that lipoprotein flux into the arterial wall is increased in people with diabetes, possibly explaining the accelerated development of atherosclerosis.
Key words: low density lipoprotein • transvascular transport • lipoprotein size • diabetes mellitus
This article has been cited by other articles:
 |
|
 |
|
  M. Benn, B. G. Nordestgaard, J. S. Jensen, and A. Tybjaerg-Hansen Polymorphisms in Apolipoprotein B and Risk of Ischemic Stroke J. Clin. Endocrinol. Metab., September 1, 2007; 92(9): 3611 - 3617. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Benn, B. G. Nordestgaard, J. S. Jensen, P. Grande, H. Sillesen, and A. Tybjaerg-Hansen Polymorphism in APOB Associated with Increased Low-Density Lipoprotein Levels in Both Genders in the General Population J. Clin. Endocrinol. Metab., October 1, 2005; 90(10): 5797 - 5803. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Jensen, B. Feldt-Rasmussen, K. Borch-Johnsen, K. S. Jensen, and B. G. Nordestgaard Increased Transvascular Lipoprotein Transport in Diabetes: Association with Albuminuria and Systolic Hypertension J. Clin. Endocrinol. Metab., August 1, 2005; 90(8): 4441 - 4445. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Benn, B. G. Nordestgaard, J. S. Jensen, K. Nilausen, H. Meinertz, and A. Tybjaerg-Hansen Mutation in Apolipoprotein B Associated with Hypobetalipoproteinemia Despite Decreased Binding to the Low Density Lipoprotein Receptor J. Biol. Chem., June 3, 2005; 280(22): 21052 - 21060. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kornerup, B. G. Nordestgaard, T. K. Jensen, B. Feldt-Rasmussen, J. P. Eiberg, K. S. Jensen, and J. S. Jensen Transendothelial exchange of low-density lipoprotein is unaffected by the presence of severe atherosclerosis Cardiovasc Res, November 1, 2004; 64(2): 337 - 345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Jensen, B. Feldt-Rasmussen, K. S. Jensen, P. Clausen, H. Scharling, and B. G. Nordestgaard Transendothelial lipoprotein exchange and microalbuminuria Cardiovasc Res, July 1, 2004; 63(1): 149 - 154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. M. Sacks and H. Campos Low-Density Lipoprotein Size and Cardiovascular Disease: A Reappraisal J. Clin. Endocrinol. Metab., October 1, 2003; 88(10): 4525 - 4532. [Full Text] [PDF]
|
|