Additional Materials

This data supplement includes supporting materials for this study.

Files in this Data Supplement:

• Supplemental Figure Legends
• Supplemental Table 3 - Methods to assay angiogenic responses in vitro
• Supplemental Table 4 - Methods to determine intracellular reactive oxygen species (ROS)
• Supplemental Table 5 - Immunohostochemical methods used
• Detailed Legends for Print Figures - Detailed Figure Legends for Illustrations Appearing in Hard-Print
• Supplemental Figure 1 - Double, but not single, transfection of HMEC with Dicer siRNA successfully down-regulated miRNA content
• Supplemental Figure 2 - Dicer knockdown induced miRNA deficiency increased VEGF expression
• Supplemental Figure 3 - Dicer knockdown induced miRNA deficiency impaired human microvascular endothelial cell migration
• Supplemental Figure 4 - Dicer knockdown induced miRNA deficiency limited ROS production under basal and activation conditions
• Supplemental Figure 5 - Significance of ROS in the angiogenic response of HMEC
• Supplemental Figure 6 - Exogenously administered H2O2 rescued Dicer knockdown HMEC from impaired Matrigel® tube formation
• Supplemental Table 1 - Primer sets used for real-time PCR
• Supplemental Table 2 - Methods for the detection of proteins