Additional Materials

This data supplement includes supporting materials for this study.

Files in this Data Supplement:

• Supplemental materials, methods, and figures - Supplemental materials and methods. Supplemental figures and detailed figure legends. Figure 1A--Native gel electrophoresis (NativePage Novex Bis-Tris gel 4-16%, Invitrogen) of 5µg human C-reactive protein (lane 1), buffer solution (lane 2), and NativeMark 8 standard proteins (lane 3) combined with Coomassie staining. Figure 1B--Native gel electrophoresis of standard proteins (lane 1), buffer (lane 2, 4, 6, 8, 10), and increasing amounts of human C-reactive protein 0.05 µg (lane 3), 0.1 µg (lane 5), 0.25 µg (lane 7), 0.5µg (lane 9) and 5µg (lane 11) followed by silver staining (SilverQuest Kit, Invitrogen). Figure 2--Expression of CD80 and CD40 after 6, 24 and 48h incubation with control buffer (CTR) or 60µg/ml CRP (n=4).