Published online before print September 7, 2006, doi: 10.1161/01.ATV.0000244680.14653.9a
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
© 2006 American Heart Association, Inc.
Brief Reviews |
Regulation of Fibrinolysis by Thrombin Activatable Fibrinolysis Inhibitor, an Unstable Carboxypeptidase B That Unites the Pathways of Coagulation and Fibrinolysis
From the Department of Molecular and Experimental Medicine (L.O.M., B.N.B.), The Scripps Research Institute, La Jolla, Calif; and the Thrombosis and Haemostasis Laboratory (B.N.B.), Department of Haematology, University Medical Center, Utrecht, The Netherlands.
Correspondence to Bonno N Bouma, PhD, The Scripps Research Institute, Department of Molecular and Experimental Medicine (MEM-180), 10550 North Torrey Pines Road, La Jolla, CA 92037. Email bouma{at}scripps.edu
 |
Abstract |
|---|
Top
Abstract
IntroductionThe coagulation and fibrinolytic systems safeguard the patency of the vasculature and surrounding tissue. Cross regulation of coagulation and fibrinolysis plays an important role in preserving a balanced hemostatic process. Identification of Thrombin Activatable Fibrinolysis Inhibitor (TAFI) as an inhibitor of fibrinolysis and one of the main intermediates between coagulation and fibrinolysis, greatly improved our understanding of cross regulation of coagulation and fibrinolysis. As TAFI is an enzyme that is activated by thrombin generated by the coagulation system, its activation is sensitive to the dynamics of the coagulation system. Defects in coagulation, such as in thrombosis or hemophilia, resonate in TAFI-mediated regulation of fibrinolysis and imply that clinical symptoms of coagulation defects are amplified by unbalanced fibrinolysis. Thrombomodulin promotes the generation of both antithrombotic activated protein C (APC) and prothrombotic (antifibrinolytic) activated TAFI, illustrating the paradoxical effects of thrombomodulin on the regulation of coagulation and fibrinolysis. This review will discuss the role of TAFI in the regulation of fibrinolysis and detail its regulation of activation and its potential therapeutic applications in thrombotic disease and bleeding disorders.
Key Words: coagulation • fibrinolysis • carboxypeptidase B • TAFI • thrombomodulin • APC • hemophilia
|
Introduction |
|---|
Thrombin Activatable Fibrinolysis Inhibitor (TAFI) is an enzyme that is activated by thrombin generated by the coagulation system and which downregulates fibrinolysis. It is, therefore, considered to play an important role in the regulation of fibrinolysis by the coagulation system. Independent identification of TAFI by different groups resulted in different nomenclature for the same protein. Consequently, activated TAFI (TAFIa), plasma carboxypeptidase B (pCPB), carboxypeptidase U (CPU), or carboxypeptidase R (CPR) are all used in literature to denote the active enzyme TAFIa, whereas TAFI, procarboxypeptidase U (pro-CPU), plasma procarboxypeptidase B (pro-pCPB), or procarboxypeptidase R (pro-CPR) are used to denotes the zymogen, TAFI.1–4 In this review TAFI will be used to indicate the zymogen and TAFIa to indicate the enzyme.
Activation of TAFI by thrombin is greatly enhanced in the presence of thrombomodulin and this presents two distinct pathways for TAFI activation: (1) by relative low concentrations of thrombin in the presence of thrombomodulin, and (2) by relative high concentrations of thrombin generated via the intrinsic pathway of coagulation in the absence of thrombomodulin. Activation of TAFI is therefore sensitive to the dynamics of coagulation and so is its inhibition of fibrinolysis. The fact that TAFIa inhibits fibrinolysis by modulating the fibrin cofactor function for plasmin generation, rather than by direct irreversible inhibition, and the fact that the activity of TAFIa is short-lived contribute to the dynamics of this system.
The discovery of TAFI greatly facilitated our understanding of cross regulation between coagulation and fibrinolysis. The redundancy in the fibrinolytic pathway hampered much of the earlier work addressing the physiological role of TAFI.5–7 Initially, only a role for TAFI in exogenous (tPA-induced) fibrinolysis could be demonstrated.8–11 Nevertheless, its (anticipated) important role in the regulation of endogenous fibrinolysis is now slowly crystallizing.12–15 Increased risks of thrombotic complications associated with an overacting coagulation pathway or the chance of bleeding accompanying coagulation defects are generally accepted. Much less appreciated is the fact that changes in thrombin formation resonate in the degree of clot protection via the activation of TAFI. For instance, to what degree is the bleeding diathesis in hemophilia patients attributable to defects in clot protection versus defects in clot formation? How much does increased activation of TAFI contribute to the elevated risk of venous thrombosis associated with high levels coagulation factors II, VIII, IX, and XI? These questions highlight the importance and complexity of cross-regulatory mechanisms for coagulation and fibrinolysis by TAFI.
Besides having a role in the regulation of fibrinolysis, TAFIa has been implicated in the inactivation of the inflammatory mediator bradykinin, the anaphylatoxins C3a and C5a, annexin II, and osteopontin.16–19 These studies suggest that TAFI may also have an important function in the regulation of inflammation, wound healing, and blood pressure (for review see refs 20–22). The focus of this review is on the role of TAFI in fibrinolysis. In particular the emphasis will be on the regulation of TAFI activation, on the role of thrombomodulin in the regulation of TAFI activation, and on the dynamic regulation of TAFI-mediated clot protection.
|
The Fibrinolytic System |
|---|
Release of tissue-type plasminogen activator (t-PA) (tPA) from endothelial cells initiates limited conversion of plasminogen to plasmin when both plasminogen and tPA bind to the fibrin surface to generate plasmin (Figure 1).23–25 Specific interactions of the lysine-binding sites in plasminogen and tPA with C-terminal lysine residues in partially degraded fibrin result in the formation of a ternary complex and increased catalytic efficiency of plasmin formation.26–28 As a result, fibrinolysis proceeds from the initial phase into the propagation phase. Plasmin subsequently degrades the fibrin network into small soluble fragments. Regulation of fibrinolysis occurs at least at three levels: (1) by inhibition of proteolytic enzymes responsible for activation of plasminogen eg, plasminogen activator inhibitors (plasminogen activator inhibitor [PAI])29; (2) by inhibition of plasmin eg,
2-antiplasmin,30 and (3) by thrombin, in response to activation of the coagulation cascade.1,31,32 Clots with a fibrinolysis-resistant architecture are formed when the initial rate of thrombin formation is relatively high. In contrast, clots formed during relatively low rates of thrombin formation are made up of thick coarse fibers that are readily degraded by plasmin. Thrombin also activates factor XIII and TAFI to stabilize the fibrin clot. Cross linking of fibrin chains and linking 2-antiplasmin to fibrin by activated factor XIII makes the fibrin clot more resistant to fibrinolysis.33,34
|
|
Inhibition of Fibrinolysis by Activated TAFI |
|---|
TAFIa inhibits fibrinolysis by abrogating plasmin-mediated auto-feedback loops designed to generate a burst of plasmin formation. Plasmin promotes its own formation in at least two ways (Figure 1). On one hand, continuous cleavage of fibrin by plasmin generates new C-terminal lysine residues that act as binding sites for plasminogen and tPA and greatly enhance the efficiency of tPA-mediated plasminogen activation. Proteolysis of these C-terminal Lysine residues from partially degraded fibrin by TAFIa abrogates the fibrin cofactor function for tPA-mediated plasminogen activation and effectively shuts down plasmin formation. On the other hand, plasmin promotes its own formation by proteolytically truncating native Glu-plasminogen to generate Lys-plasminogen. Lys-plasminogen is a better substrate for tPA and it does not have the stringent requirement for partially degraded fibrin (C-terminal Lys) as a cofactor in the activation by tPA.35 The diminished cofactor role of partially degraded fibrin in the activation of Lys-plasminogen is most likely the reason why the activation of Lys-plasminogen is not (less) susceptible to downregulation by TAFIa.1,36 Therefore, on the generation of Lys-plasminogen fibrinolysis is no longer opposed by TAFIa and proceeds into the propagation phase (Figure 1). Nevertheless, TAFIa prevents the progression of fibrinolysis into the propagation phase by directly inhibiting the conversion of Glu to Lys-plasminogen by abrogating the fibrin cofactor function and indirectly by inhibition of plasmin formation from Glu-plasminogen.36
Recent data from two groups indicate that TAFI affects the clot dissolution through a threshold-dependent mechanism: as long as the TAFIa concentration remains above the threshold, TAFIa prevents the progression of lysis into the propagation phase.37,38 Removal of the available plasminogen binding sites on fibrin combined with the inhibition of Lys-plasminogen formation are the basis for this phenomenon. From the moment the TAFIa concentration drops below its threshold value, plasminogen binding sites become available, plasmin is generated and via plasmin-mediated feedback the number of C-terminal lysine residues increases exponentially. Plasmin can now truncate native Glu-plasminogen into Lys-plasminogen, which is not only a better substrate for tPA but its activation is no longer under control by TAFIa.1,35 Thus, a TAFIa concentration below the threshold results in an accelerated rate of lysis and abrogation of TAFIa-mediated inhibition of lysis. This threshold value for TAFIa-mediated inhibition of fibrinolysis is dependent on the rate of TAFIa formation, the intrinsic instability of TAFIa, the presence of plasmin inhibitors, and the tPA concentration.
Although the discussion in this review is limited to tPA-mediated fibrinolysis, TAFIa also inhibits fibrinolysis initiated by other plasminogen activators (such as urokinase).39 In addition, TAFIa inhibits plasminogen binding and activation on cells suggesting a possible role for TAFI in cellular fibrinolysis and possibly inflammation as well.40
|
Activation of TAFI by the Extrinsic and Intrinsic Coagulation Pathway |
|---|
Activation of TAFI by thrombin is an inefficient process and requires relatively high concentrations of thrombin. At high tissue factor concentration, the initial generation of factor Xa exceeds inhibition by its inhibitors tissue factor pathology inhibitor (TFPI) and ATIII and thrombin formation continues inside the fibrin clot (Figure 2). In contrast, at low tissue factor concentrations, when generation of factor Xa is limited, thrombin-catalyzed activation of factor XI continues the generation of thrombin via the intrinsic pathway after clot formation. Although only small amounts of factor XIa are initially formed, the intrinsic pathway, because of the continued activation of factor XI by thrombin and the amplification power of both the tenase and the prothrombinase complex, provides for a secondary burst of thrombin formation. These relatively high concentrations of thrombin are required for the activation of TAFI and contrast the small initial amounts of thrombin that are sufficient for fibrin formation. Thus dependent on the concentration of tissue factor during initiation of coagulation the secondary burst of thrombin formation required for activation of TAFI is generated by the extrinsic pathway (high tissue factor concentrations) or the intrinsic pathway (low tissue factor concentrations) (Figure 2). Although TAFIa-mediated prolongation of the clot lysis is indifferent as to how the thrombin is generated and the degree of downregulation of fibrinolysis remains the same over a broad range of tissue factor concentrations (Figure 3), the pathways responsible for TAFI activation shift from extrinsic pathway–mediated TAFI activation to intrinsic pathway–mediated TAFI activation with decreasing concentrations of tissue factor. The thrombin requirements for TAFI activation are different in the presence of thrombomodulin as discussed below.
|
|
) and absence (
) of the TAFIa inhibitor (CPI) from potato tubers represents inhibition of fibrinolysis by TAFIa. Disruption of thrombin formation via the intrinsic pathway by a blocking antibody against factor XI (
) decreased the clot lysis time at low tissue factor concentrations (<0.1 pmol/L), indicating dependence of TAFI activation on the intrinsic pathway. In contrast, the clot lysis time at higher tissue factor concentrations is not affected. This indicates that TAFIa-mediated prolongation of the clot lysis remains stable over a broad range of tissue factor concentrations, but that the pathway responsible for TAFI activation shift from extrinsic pathway-mediated TAFI activation to intrinsic pathway-mediated TAFI activation at lower tissue factor concentrations. Modified from Mosnier et al. |
Activation of TAFI by Thrombin–Thrombomodulin |
|---|
The endothelial cell receptor thrombomodulin stimulates the activation of TAFI by thrombin
1250-fold and eliminates the need for high thrombin concentrations.41 In the presence of thrombomodulin, the activation of TAFI is therefore independent of thrombin generation via the intrinsic pathway. Accordingly, thrombomodulin corrects the premature lysis of clots from factor X-, IX-, VIII-, and XI-deficient plasmas in vitro and potentially provides an additional way to correct the hyper fibrinolysis in patients with hemophilia (see below).41–43 The cofactor role of thrombomodulin in the activation of TAFI and regulation of fibrinolysis by TAFI adds an additional layer of complexity as thrombomodulin is also an essential cofactor for thrombin-mediated activation of anticoagulant protein C. APC, together with its cofactor protein S, inactivates the coagulation cofactors Va and VIIIa that are required for efficient thrombin generation. It is via inhibition of thrombin formation and subsequent decreased TAFI activation that APC exerts its profibrinolytic effect (Figure 4).44,45 This profibrinolytic effect of the protein C pathway emphasizes the duplicitous role of thrombomodulin in the regulation of protein C and TAFI activation. On one hand, thrombomodulin downregulates fibrinolysis by stimulation of TAFI activation but on the other hand, thrombomodulin upregulates fibrinolysis by stimulation of protein C activation resulting in the inhibition of TAFI activation (Figure 4). Support for in vivo relevance of the profibrinolytic effects of APC comes from a mouse model for thrombin-induced thromboembolism in which APC protects from thrombin-induced thromboembolism by rendering the formed fibrin more susceptible to plasmin degradation via inhibition of thrombin formation required for activation of TAFI.46
|
The factor V R506Q (factor V Leiden) mutation abolishes a cleavage site for APC, thereby attenuating the inactivation of factor Va resulting in increased thrombin formation.47 R506Q factor V, therefore, leads to an increased rate of activation of TAFI and downregulation of fibrinolysis.45,48 Impaired pulmonary clot lysis in mice expressing factor V R506Q confirmed the link between R506Q factor V and impaired fibrinolysis.49 On the other hand, inhibition of endogenous soluble thrombomodulin in plasmas of factor XI–deficient patients reduced APC-mediated downregulation of TAFI activation and increased TAFI-mediated clot protection.50 These studies emphasize the delicate balance between procoagulant (antifibrinolytic) and anticoagulant (profibrinolytic) properties of the coagulation system.
|
Regulation of Pro- and Antifibrinolytic Effects of Thrombomodulin |
|---|
The relative concentration of thrombomodulin is an important factor in the regulation of profibrinolytic (stimulation of protein C activation) and antifibrinolytic (stimulation of TAFI activation) effects by thrombomodulin. We found that at relative low thrombomodulin concentrations, fibrinolysis is downregulated by stimulation of TAFI activation, whereas the profibrinolytic effects of protein C activation becomes more pronounced at relative high concentrations of thrombomodulin.36 Inhibition of thrombin formation by APC then limits TAFI activation. Therefore, stimulation of protein C activation at relatively high concentrations of thrombomodulin results in an upregulation of fibrinolysis.45 The basis for the different thrombomodulin requirements is most likely in the fact that thrombomodulin directly stimulates TAFI activation, whereas inhibition of TAFI activation via stimulation of protein C activation by thrombomodulin is indirect. Thus, indirect inhibition of TAFI activation via protein C activation requires a more robust activation by thrombin–thrombomodulin than the direct activation of TAFI by thrombin–thrombomodulin. Competition between protein C and TAFI at physiological concentrations is not anticipated to make a significant difference given the similar activation kinetics of TAFI (KM=0.6 to 1 µmol/L; kcat 0.5 to 1.2=sec–1; plasma concentration=70 to 275 nM)41,51,52 and protein C (KM=1 to 8 µmol/L; kcat 250=sec–1; plasma concentration=65 nM)53,54 by the thrombin–thrombomodulin complex and their plasma concentrations of 5 to 50-fold below KM values.55
Endothelial cells derived from different tissues/organs display considerable heterogeneity in their expression of thrombomodulin.56 Expression of thrombomodulin is dependent on the extracellular environment, the components of the extracellular matrix, and the surface to blood ratio of the vessel. Hence, the effective thrombomodulin concentration on endothelial cells can rise from less than 1 nM in the large arteries to more than 100 nM in the capillary beds.56,57 The paradoxical effects of thrombomodulin imply that TAFI is more readily activated in the larger blood vessels with relatively low thrombomodulin expression, whereas TAFI activation in the smaller capillaries with relatively high thrombomodulin expression is inhibited by APC generation (Figure 5). However, this does not take into account the presence of additional cofactors for protein C generation and function and other modulators of thrombomodulin availability.
|
The endothelial protein C receptor (EPCR) is such an important cofactor for protein C activation as it enhances protein C activation by thrombin–thrombomodulin up to 20-fold.58 Physiological activation of protein C by thrombin occurs on the endothelial cell surface, and localization of protein C on the endothelial surface by binding to EPCR greatly facilitates activation of protein C by the thrombin–thrombomodulin complex.54 As the expression pattern of EPCR is opposite to that of thrombomodulin, in that EPCR is relatively abundant on larger vessels with low thrombomodulin expression but its expression on small capillaries is much lower, it is suggested that EPCR allows for efficient protein C activation on larger vessels where the thrombomodulin concentration is relatively low.59,60 Thus, in the presence of EPCR profibrinolytic effects of APC will be more pronounced at lower thrombomodulin concentration because of more efficient generation of APC in the presence of EPCR (Figure 5).
Platelet factor 4 (PF4) is released from the alpha granules on platelet activation and is a soluble cofactor for protein C activation. PF4 enhances protein C activation both in vitro and in vivo, presumably via the formation of an electrostatic bridge between protein C and thrombomodulin.61,62 In contrast to stimulation of protein C activation, PF4 inhibited the activation of TAFI by the thrombin–thrombomodulin complex, indicating that PF4 enhances profibrinolytic effects of thrombomodulin (Figure 5).63 PF4 did not interfere with thrombin-mediated TAFI activation in the absence of thrombomodulin. This suggests that PF4 selectively promotes thrombomodulin-mediated profibrinolytic effects and shift the pathway responsible for TAFI activation from thrombomodulin-dependent toward thrombin-dependent, either generated via the extrinsic pathway or the intrinsic pathway.
Protein C inhibitor (PCI) functions in plasma as an effective inhibitor of thrombin in complex with thrombomodulin. Inhibition of thrombin by PCI is enhanced by two orders of magnitude in the presence of thrombomodulin.64,65 PCI-mediated inhibition of thrombin bound to thrombomodulin decreases the availability of thrombin–thrombomodulin complexes and thereby the activation of protein C and TAFI.66,67 Because the effects of thrombomodulin-dependent protein C and TAFI activation on fibrinolysis are dependent on the thrombomodulin concentration, the effects of PCI on fibrinolysis are similarly dependent on the thrombomodulin concentration. At low thrombomodulin concentrations, when antifibrinolytic effects of thrombomodulin dominate, PCI has a profibrinolytic effect via inhibition of TAFI activation by thrombin–thrombomodulin. In contrast, at high concentrations of thrombomodulin, PCI has an antifibrinolytic effect. This antifibrinolytic effect of PCI is attributable to inhibition of protein C activation, which results in increased thrombin formation, and increased TAFI activation. Hence, the antifibrinolytic effect of PCI (Figure 5).
Protein S is a physiological important cofactor for APC anticoagulant activity in the inactivation of factors Va and VIIIa.68 In addition to its cofactor role for APC anticoagulant activity, protein S has also an APC-independent direct anticoagulant effect that was recently suggested to involve stimulation of TFPI-mediated inhibition of tissue factor induced thrombin formation.69 Accordingly, protein S has two effects on TAFI activation and TAFIa-mediated downregulation of fibrinolysis. The direct anticoagulant activity of protein S prolongs the lag-time of TAFI activation, whereas the APC cofactor function of protein S deceases the amplitude of TAFI activation.70 Collectively, these two effects of protein S on TAFI activation stimulate fibrinolysis markedly (Figure 5).
These examples highlight the complexity of TAFI activation and the regulation of fibrinolysis by TAFI. Notwithstanding, our current understanding of this system has led to the appreciation of coagulation and fibrinolysis crossregulation, and this will improve our ability to anticipate the effects of crossregulation in various diseases. One such area where our evolving understanding of TAFI-mediated cross regulation of coagulation and fibrinolysis could benefit is hemophilia.
|
Antifibrinolytic Treatment for Hemophilia |
|---|
Bleeding in hemophiliacs occurs predominantly hours or days after injury despite normal plasma levels of factors VII and X.71 These phenomena have been attributed to defective fibrin formation, but these observations also point to a potential role for impaired downregulation of fibrinolysis by TAFIa in the bleeding manifestation of hemophiliacs. Indeed, in a controlled trial of antifibrinolytic therapy in hemophilia, patients undergoing dental extractions characteristically bled from tooth sockets after 3 to 5 days, whereas bleeding was completely prevented in patients receiving the antifibrinolytic agent
-aminocaproic acid (ACA).72 This lysine analogue acts very similar to TAFIa in that it inhibits plasmin formation by preventing the binding of plasminogen to C-terminal Lysine residues on partially degraded fibrin that are required for efficient plasmin formation. These observations support the idea that the severe bleeding disorder in hemophilia patients may be attributable to triple defect: (1) reduced thrombin generation via the extrinsic pathway at low tissue factor concentrations; (2) a reduced secondary burst of thrombin generation via the intrinsic pathway, and (3) a defective downregulation of the fibrinolytic system by the intrinsic pathway.42,43 This triple defect results not only in an inadequate hemostatic response for initial clot formation but also in a lysis susceptible clot when a fibrin clot is eventually formed. Thrombomodulin corrected the premature lysis of clots from plasmas deficient in factors of the intrinsic pathway via stimulation of TAFI activation and provided, at least in vitro, support for the idea that stimulation of TAFI activation might help prevent bleeding in hemophilia.41–43 Application of thrombomodulin in hemophilia seems counter intuitive because of its anticoagulant effect. The use of variants of thrombomodulin that lack the ability to generate APC could, at least theoretically, overcome most of the undesired anticoagulant effects of thrombomodulin.73
Recombinant factor VIIa is a generally accepted treatment for hemophilia A and B patients with inhibitory antibodies. Factor VIIa not only improves clot formation but also improves TAFIa-dependent clot protection.74 Concentrations of factor VIIa required to normalize TAFIa-dependent clot protection varied greatly in individual plasmas of hemophilia A patients, a phenomenon that could be attributed to variations in TFPI levels. Molecular engineering of factor VIIa variants with increased catalytic activity greatly improved efficiency of factor VIIa-mediated stimulation of TAFI activation as the concentration of factor VIIa required for normalization of TAFIa-dependent clot protection decreased by two orders of magnitude.75 Therefore, enhanced inhibition of fibrinolysis by TAFI in hemophilia patients receiving recombinant factor VIIa is likely to contribute to the prevention of bleeding complications in these patients.
TAFI levels in plasma correlate with TAFIa-mediated inhibition of fibrinolysis in normal individuals, indicating that activation of TAFI by thrombin is dependent on its concentration.51 Extrapolation of this phenomenon to hemophilia A suggests that increasing the TAFI concentration should improve TAFIa-mediated inhibition of fibrinolysis in these patient plasmas. Indeed, a two-fold increase of the TAFI plasma concentration resulted in a normalization of TAFIa-dependent clot protection.43 This suggests that hemophilia patients could potentially benefit from therapeutic application of TAFI to enhance clot stability. Recent advances in molecular engineering of TAFI variants with increased enzymatic stability have reignited an interest for antifibrinolytic therapeutic applications for TAFI.
|
Advances in Molecular Engineering of TAFIa Variants |
|---|
The enzymatic activity of TAFIa is unstable and highly sensitive to temperature (the half-life of TAFIa at 37°C is
10 minutes, 1 to 2 hours at 22°C, and several hours at 4°C).52 Inactivation of TAFIa is caused by a spontaneous conformational change. Proteolytic inactivation of TAFIa is not involved in decay of TAFIa enzymatic activity as mutagenesis of the sites responsible for proteolytic degradation of TAFIa (Arg302 for thrombin and Lys327 and/or Arg330 for plasmin) does not prevent the decay of TAFIa activity, although it prevents proteolytic degradation.76–78 In fact, mutation at these residues further decreased the stability of enzymatic activity of TAFIa. A naturally occurring TAFI polymorphism encodes a TAFI isoform (Thr325Ile) that after activation has a 2-fold prolonged half life compared with its counterpart (Thr325). Accordingly, Ile325-TAFIa inhibited fibrinolysis 50% more effectively than Thr325-TAFIa, both in the presence and absence of thrombomodulin. This confirms that TAFI variants with a prolonged half-life inhibit fibrinolysis more effectively. It also indicates that a relatively small structural change in TAFI, such as a single amino acid change, can have marked effects on the enzymatic stability and suggests that molecular engineering approaches to construct stable TAFIa variants are ultimately feasible.
Attempts to stabilize TAFIa were first directed toward exploration of the homology with the enzymatic stable carboxypeptidase B from pancreas (CPB). Pancreatic CPB is 40% identical to TAFIa (note: plasma CPB [pCPB] is 100% identical to TAFIa) but in contrast to TAFIa its enzymatic activity is stable over time. Construction of a TAFI-CPB chimera (TAFI containing residues 293 to 401 of pancreatic CPB) increased the half-life of TAFIa enzymatic activity by 10-fold.79 Unfortunately, the antifibrinolytic activity of the chimeric molecule was severely compromised.
Recent molecular engineering approaches identified several residues that either alone or in combination greatly increase the TAFIa enzymatic activity without compromising antifibrinolytic activity.80,81 TAFIa variants Ser305Cys-Thr325Ile-Thr329Ile and Ser305Cys-His333Tyr-His335Gln have a prolonged half-life of 10- and 25-fold, respectively. These residues are generally located in a region (residues 300 to 340) that was previously suspected to mediate the enzymatic stability of TAFIa. Although the molecular mechanism behind TAFIa’s instability remains elusive, it is likely that the flexibility of the -helix and connecting segments (blue residues, 300 to 340; Figure 6) affects the orientation of the substrate binding residues Tyr341 and Asp349 and thus TAFIa enzymatic activity. The most stable TAFIa variant (Ser305Cys-His333Tyr-His335Gln, T1/25.5 hour) to date is still less stable than carboxypeptidase B from pancreas, but continuing effort will no doubt result in even more stable TAFIa variants. As for correcting the premature lysis of clots in hemophilia, further studies will be needed to determine the optimal stability of TAFIa for prevention of bleeding as ultimately stable TAFI also conveys a possible risk for thrombotic complications.
|
|
Clinical Implications in Relation to Possible Therapeutic Applications for TAFI |
|---|
Treatment of Bleeding Disorders
A potential complication of the therapeutic application of TAFI in the treatment of bleeding disorders would be the inappropriate inhibition of fibrinolysis by increase in TAFI levels or by the use of stable TAFIa variants that may lead to thrombotic complications. High TAFI levels (above the 90th percentile) were associated with a mild increased risk for venous thrombosis.82 Elevated levels of factors II, VIII, IX, and XI are also associated with mild increased risks for venous thrombosis and upregulated thrombin-mediated TAFI activation might contribute to the increased risk for thrombosis.83–86 In contrast, elevated TAFI levels seemed to convey a decreased risk for arterial thrombosis.87,88 This remarkable observation prompted the hypothesis that the antiinflammatory activities of TAFI via inactivation of complement mediators C3a and C5a and protection against bradykinin-induced hypotension might contribute to a decreased risk for arterial thrombosis (for review see refs 21,89). This hypothesis is further supported by the observation that patients with factor XI deficiency are not protected against arterial thrombosis.90 Because of the impaired activation of TAFI via the intrinsic pathway, these patients may lack the potential beneficial effects of TAFIa on the inhibition of inflammation, which plays a role in the early development of atherosclerosis. These observations illustrate our limited understanding of the interactions between TAFI and thrombosis, but also suggest that major thrombotic complications associated with therapeutic application of TAFI or stable TAFIa variants is not anticipated.
Thrombolytic Therapy
Inhibition of TAFIa by inhibitors (such as CPI from potato tubers) increased the efficiency of tPA-mediated thrombolysis in several in vivo animal models and accelerated the development of novel inhibitors for TAFIa.8–11 An alternative or adjuvant approach is to target the activation of TAFI by using inhibitors of factor XIa. The advantage of factor XIa inhibitors over, eg, thrombin inhibitors, is that inhibition of factor XIa inhibits the generation of thrombin via the intrinsic pathway responsible for clot protection while leaving the extrinsic pathway responsible for clot formation intact. Presumably, the risk of bleeding associated with inhibition of factor XIa is less compared with the bleeding risk conveyed by the use of thrombin inhibitors. However, one should keep in mind that inhibition of TAFI activity and/or activation during thrombolysis might also result in inhibition of the beneficial effects of TAFI on inflammation (C3a, C5a, and bradykinin) and other yet unknown substrates. It may be worthwhile to pay attention to the specificity of these novel inhibitors for the different TAFIa substrates. Ideally, such thrombolytic TAFIa inhibitor will inhibit downregulation of fibrinolysis by TAFIa without affecting its reactivity toward small peptides in order to preserve antiinflammatory effects of TAFI.
|
Future Directions and Conclusions |
|---|
The discovery of TAFI greatly facilitated our understanding of crossregulation between coagulation and fibrinolysis. Our understanding of the regulation of TAFI activation and activity is evolving and aids in the unraveling of the role for TAFI in health and disease. The possibilities of therapeutic applications of TAFIa inhibitors for the improvement of thrombolysis are promising. Recent advancements in the engineering of TAFI variants with increased enzymatic stability have given new hope for possible applications where inhibition of fibrinolysis would be beneficial such as for hemophilia.
|
Acknowledgments |
|---|
We apologize to our colleagues whose work could not be cited because of limitations of space, but have contributed to our current views and understanding of the regulation and interactions of these pathways. We thank Dr Vendrell (University of Barcelona, Spain) for providing the coordinates of the TAFI homology model.
Source of Funding
This work was supported, in part, by the American Society of Hematology (Basic Research Scholar Award to L.O.M.).
Disclosures
None.
|
Footnotes |
|---|
Original received June 5, 2006; final version accepted August 24, 2006.
|
References |
|---|
1. Bajzar L, Manuel R, Nesheim ME. Purification and characterization of TAFI, a thrombin-activable fibrinolysis inhibitor. J Biol Chem. 1995; 270: 14477–14484.
2. Eaton DL, Malloy BE, Tsai SP, Henzel W, Drayna D. Isolation, molecular cloning, and partial characterization of a novel carboxypeptidase B from human plasma. J Biol Chem. 1991; 266: 21833–21838.
3. Wang W, Hendriks DF, Scharpé SS. Carboxypeptidase U, a plasma carboxypeptidase with high affinity for plasminogen. J Biol Chem. 1994; 269: 15937–15944.
4. Campbell W, Okada H. An arginine specific carboxypeptidase generated in blood during coagulation or inflammation which is unrelated to carboxypeptidase N or its subunits. Biochem Biophys Res Commun. 1989; 162: 933–939.[CrossRef][Medline] [Order article via Infotrieve]
5. Nagashima M, Yin ZF, Zhao L, White K, Zhu Y, Lasky N, Halks-Miller M, Broze GJ Jr, Fay WP, Morser J. Thrombin-activatable fibrinolysis inhibitor (TAFI) deficiency is compatible with murine life. J Clin Invest. 2002; 109: 101–110.[CrossRef][Medline] [Order article via Infotrieve]
6. Wagenaar GT, Girma M, Havik SR, Voskuilen MC, Bouma BN, and Meijers JCM. Generation and characterization of thrombin activatable fibrinolysis inhibitor deficient mice (Abstract). Thromb Haemost. 2001; Suppl: OC1759.
7. Biemond BJ, Havik SR, and Meijers JCM. Absence of enhanced lysis of pulmonary microemboli in TAFI deficient mice. Blood. 2001; 98: 255. Abstract.
8. Minnema MC, Friederich PW, Levi M, Von dem Borne PAK, Mosnier LO, Meijers JCM, Biemond BJ, Hack CE, Bouma BN, ten Cate H. Enhancement of rabbit jugular vein thrombolysis by neutralization of factor XI - In vivo evidence for a role of factor XI as an anti-fibrinolytic factor. J Clin Invest. 1998; 101: 10–14.[Medline] [Order article via Infotrieve]
9. Nagashima M, Werner M, Wang M, Zhao L, Light DR, Pagila R, Morser J, Verhallen P. An inhibitor of activated thrombin-activatable fibrinolysis inhibitor potentiates tissue-type plasminogen activator-induced thrombolysis in a rabbit jugular vein thrombolysis model. Thromb Res. 2000; 98: 333–342.[CrossRef][Medline] [Order article via Infotrieve]
10. Refino CJ, DeGuzman L, Schmitt D, Smyth R, Jeet S, Lipari MT, Eaton DL, Bunting S. Consequences of inhibition of plasma carboxypeptidase B on in vivo thrombolysis, thrombosis and hemostasis. Fibrinolysis Proteolysis. 2000; 14: 305–314.[CrossRef]
11. Klement P, Liao P, Bajzar L. A novel approach to arterial thrombolysis. Blood. 1999; 94: 2735–2743.
12. Swaisgood CM, Schmitt D, Eaton D, Plow EF. In vivo regulation of plasminogen function by plasma carboxypeptidase B. J Clin Invest. 2002; 110: 1275–1282.[CrossRef][Medline] [Order article via Infotrieve]
13. te Velde EA, Wagenaar GT, Reijerkerk A, Roose-Girma M, Borel-Rinkes I, Voest EE, Bouma BN, Gebbink MF, Meijers JCM. Impaired healing of cutaneous wounds and colonic anastomoses in mice lacking thrombin-activatable fibrinolysis inhibitor. J Thromb Haemost. 2003; 1: 2087–2096.[CrossRef][Medline] [Order article via Infotrieve]
14. Mao SS, Holahan MA, Bailey C, Wu G, Colussi D, Carroll SS, Cook JJ. Demonstration of enhanced endogenous fibrinolysis in thrombin activatable fibrinolysis inhibitor-deficient mice. Blood Coagul Fibrinolysis. 2005; 16: 407–415.[Medline] [Order article via Infotrieve]
15. Renckens R, Roelofs JJ, Ter Horst SA, van ’t Veer C, Havik SR, Florquin S, Wagenaar GT, Meijers JCM, van der Poll T. Absence of thrombin-activatable fibrinolysis inhibitor protects against sepsis-induced liver injury in mice. J Immunol. 2005; 175: 6764–6771.
16. Shinohara T, Sakurada C, Suzuki T, Takeuchi O, Campbell W, Ikeda S, Okada N, Okada H. Pro-carboxypeptidase R cleaves bradykinin following activation. Int Arch Allergy Immunol. 1994; 103: 400–404.[Medline] [Order article via Infotrieve]
17. Myles T, Nishimura T, Yun TH, Nagashima M, Morser J, Patterson AJ, Pearl RG, Leung LL. Thrombin activatable fibrinolysis inhibitor, a potential regulator of vascular inflammation. J Biol Chem. 2003; 278: 51059–51067.
18. Campbell WD, Lazoura E, Okada N, Okada H. Inactivation of C3a and C5a octapeptides by carboxypeptidase R and carboxypeptidase N. Microbiol Immunol. 2002; 46: 131–134.[Medline] [Order article via Infotrieve]
19. Fogg DK, Bridges DE, Cheung KK, Kassam G, Filipenko NR, Choi KS, Fitzpatrick SL, Nesheim ME, Waisman DM. The p11 subunit of annexin II heterotetramer is regulated by basic carboxypeptidase. Biochemistry. 2002; 41: 4953–4961.[CrossRef][Medline] [Order article via Infotrieve]
20. Bajzar L, Jain N, Wang P, Walker JB. Thrombin activatable fibrinolysis inhibitor: not just an inhibitor of fibrinolysis. Crit Care Med. 2004; 32: S320–S324.[CrossRef][Medline] [Order article via Infotrieve]
21. Bouma BN, Mosnier LO. Thrombin activatable fibrinolysis inhibitor (TAFI) at the interface between coagulation and fibrinolysis. Pathophysiol Haemost Thromb. 2003; 33: 375–381.[CrossRef][Medline] [Order article via Infotrieve]
22. Marx PF. Thrombin-activatable fibrinolysis inhibitor. Curr Med Chem. 2004; 11: 2335–2348.[Medline] [Order article via Infotrieve]
23. Rosnobelet C, Vischer UM, Gerhard RD, Irminger JC, Halban PA, Kruithof EK. Storage of tissue-type plasminogen activator in Weibel-Palade bodies of human endothelial cells. Arterioscler Thromb Vasc Biol. 1999; 19: 1796–1803.
24. Wall U, Jern C, Jern S. High capacity for tissue-type plasminogen activator release from vascular endothelium in vivo. J Hypertens. 1997; 15: 1641–1647.[CrossRef][Medline] [Order article via Infotrieve]
25. Lijnen HR, Bachmann F, Collen D, Ellis V, Pannekoek H, Rijken DC, Thorsen S. Mechanisms of plasminogen activation. J Intern Med. 1994; 236: 415–424.[Medline] [Order article via Infotrieve]
26. Hoylaerts M, Rijken DC, Lijnen HR, Collen D. Kinetics of the activation of plasminogen by tissue plasminogen activator. Role of fibrin. J Biol Chem. 1982; 257: 2912–2919.
27. Christensen U. C-terminal lysine residues of fibrinogen fragments essential for binding to plasminogen. FEBS Lett. 1985; 182: 43–46.[CrossRef][Medline] [Order article via Infotrieve]
28. Fleury V, Angles Cano E. Characterization of the binding of plasminogen to fibrin surfaces: the role of carboxy-terminal lysines. Biochemistry. 1991; 30: 7630–7638.[CrossRef][Medline] [Order article via Infotrieve]
29. Nordt TK, Peter K, Ruef J, Kubler W, Bode C. Plasminogen activator inhibitor type-1 (PAI-1) and its role in cardiovascular disease. Thromb Haemost. 1999; 82 (Suppl 1): 14–18.[Medline] [Order article via Infotrieve]
30. Wiman B Human 2-antiplasmin. Methods Enzymol. 1981; 80 Pt C: 395–408.[CrossRef]
31. Muszbek L, Yee VC, Hevessy Z. Blood coagulation factor XIII: structure and function. Thromb Res. 1999; 94: 271–305.[CrossRef][Medline] [Order article via Infotrieve]
32. Von dem Borne PAK, Bajzar L, Meijers JCM, Nesheim ME, Bouma BN. Thrombin-mediated activation of factor XI results in a thrombin-activatable fibrinolysis inhibitor-dependent inhibition of fibrinolysis. J Clin Invest. 1997; 99: 2323–2327.[Medline] [Order article via Infotrieve]
33. Sakata Y, Aoki N. Significance of cross-linking of alpha 2-plasmin inhibitor to fibrin in inhibition of fibrinolysis and in hemostasis. J Clin Invest. 1982; 69: 536–542.[Medline] [Order article via Infotrieve]
34. Lorand L. Sol Sherry Lecture in Thrombosis: research on clot stabilization provides clues for improving thrombolytic therapies. Arterioscler Thromb Vasc Biol. 2000; 20: 2–9.
35. Miles LA, Castellino FJ, Gong Y. Critical role for conversion of Glu-plasminogen to Lys-plasminogen for optimal stimulation of plasminogen activation on cell surfaces. Trends Cardiovasc Med. 2003; 13: 21–30.[CrossRef][Medline] [Order article via Infotrieve]
36. Wang W, Boffa PB, Bajzar L, Walker JB, Nesheim ME. A study of the mechanism of inhibition of fibrinolysis by activated thrombin-activable fibrinolysis inhibitor. J Biol Chem. 1998; 273: 27176–27181.
37. Leurs J, Nerme V, Sim Y, Hendriks D. Carboxypeptidase U (TAFIa) prevents lysis from proceeding into the propagation phase through a threshold-dependent mechanism. J Thromb Haemost. 2004; 2: 416–423.[CrossRef][Medline] [Order article via Infotrieve]
38. Walker JB, Bajzar L. The intrinsic threshold of the fibrinolytic system is modulated by basic carboxypeptidases, but the magnitude of the antifibrinolytic effect of activated thrombin-activable fibrinolysis inhibitor is masked by its instability. J Biol Chem. 2004; 279: 27896–27904.
39. Mutch NJ, Moore NR, Wang E, Booth NA. Thrombus lysis by uPA, scuPA and tPA is regulated by plasma TAFI. J Thromb Haemost. 2003; 1: 2000–2007.[CrossRef][Medline] [Order article via Infotrieve]
40. Redlitz A, Tan AK, Eaton DL, Plow EF. Plasma carboxypeptidases as regulators of the plasminogen system. J Clin Invest. 1995; 96: 2534–2538.[Medline] [Order article via Infotrieve]
41. Bajzar L, Morser J, Nesheim ME. TAFI, or plasma procarboxypeptidase B, couples the coagulation and fibrinolytic cascades through the thrombin-thrombomodulin complex. J Biol Chem. 1996; 271: 16603–16608.
42. Broze GJ Jr, Higuchi DA. Coagulation-dependent inhibition of fibrinolysis: role of carboxypeptidase-U and the premature lysis of clots from hemophilic plasma. Blood. 1996; 88: 3815–3823.
43. Mosnier LO, Lisman T, van den Berg HM, Nieuwenhuis HK, Meijers JCM, Bouma BN. The defective down regulation of fibrinolysis in haemophilia A can be restored by increasing the TAFI plasma concentration. Thromb Haemost. 2001; 86: 1035–1039.[Medline] [Order article via Infotrieve]
44. Bajzar L, Nesheim ME, Tracy PB. The profibrinolytic effect of activated protein C in clots formed from plasma is TAFI-dependent. Blood. 1996; 88: 2093–2100.
45. Mosnier LO, Meijers JCM, Bouma BN. Regulation of fibrinolysis in plasma by TAFI and protein C is dependent on the concentration of thrombomodulin. Thromb Haemost. 2001; 85: 5–11.[Medline] [Order article via Infotrieve]
46. Gresele P, Momi S, Berrettini M, Nenci GG, Schwarz HP, Semeraro N, Colucci M. Activated human protein C prevents thrombin-induced thromboembolism in mice—Evidence that activated protein C reduces intravascular fibrin accumulation through the inhibition of additional thrombin generation. J Clin Invest. 1998; 101: 667–676.[Medline] [Order article via Infotrieve]
47. Bertina RM, Koeleman BPC, Koster T, Rosendaal FR, Dirven RJ, de Ronde H, van der Velden PA, Reitsma PH. Mutations in blood coagulation factor V associated with resistance to activated protein C. Nature. 1994; 369: 64–67.[CrossRef][Medline] [Order article via Infotrieve]
48. Bajzar L, Kalafatis M, Simioni P, Tracy PB. An antifibrinolytic mechanism describing the prothrombotic effect associated with factor VLeiden. J Biol Chem. 1996; 271: 22949–22952.
49. Parker AC, Mundada LV, Schmaier AH, Fay WP. Factor V Leiden inhibits fibrinolysis in vivo. Circulation. 2004; 110: 3594–3598.
50. Dai L, Mitchell M, Savidge G, Alhaq A. The profibrinolytic effect of plasma thrombomodulin in factor XI deficiency and its implications in hemostasis. J Thromb Haemost. 2004; 2: 2200–2204.[CrossRef][Medline] [Order article via Infotrieve]
51. Mosnier LO, Von dem Borne PAK, Meijers JCM, Bouma BN. Plasma TAFI levels influence the clot lysis time in healthy individuals in the presence of an intact pathway of coagulation. Thromb Haemost. 1998; 80: 829–835.[Medline] [Order article via Infotrieve]
52. Boffa MB, Wang W, Bajzar L, Nesheim ME. Plasma and recombinant thrombin-activable fibrinolysis inhibitor (TAFI) and activated TAFI compared with respect to glycosylation, thrombin/thrombomodulin-dependent activation, thermal stability, and enzymatic properties. J Biol Chem. 1998; 273: 2127–2135.
53. Esmon CT, Esmon NL, Le Bonniec BF, Johnson AE. Protein C activation. Methods Enzymol. 1993; 222: 359–385.[Medline] [Order article via Infotrieve]
54. Stearns-Kurosawa DJ, Kurosawa S, Mollica JS, Ferrell GL, Esmon CT. The endothelial cell protein C receptor augments protein C activation by the thrombin-thrombomodulin complex. Proc Natl Acad Sci U S A. 1996; 93: 10212–10216.
55. Kokame K, Zheng XL, Sadler JE. Activation of thrombin-activable fibrinolysis inhibitor requires epidermal growth factor-like domain 3 of thrombomodulin and is inhibited competitively by protein C. J Biol Chem. 1998; 273: 12135–12139.
56. Aird WC. Mechanisms of endothelial cell heterogeneity in health and disease. Circ Res. 2006; 98: 159–162.
57. Esmon CT. The roles of protein C and thrombomodulin in the regulation of blood coagulation. J Biol Chem. 1989; 264: 4743–4746.
58. Fukudome K, Esmon CT. Identification, cloning, and regulation of a novel endothelial cell protein C/activated protein C receptor. J Biol Chem. 1994; 269: 26486–26491.
59. Fukudome K, Ye X, Tsuneyoshi N, Tokunaga O, Sugawara K, Mizokami H, Kimoto M. Activation mechanism of anticoagulant protein C in large blood vessels involving the endothelial cell protein C receptor. J Exp Med. 1998; 187: 1029–1035.
60. Ye X, Fukudome K, Tsuneyoshi N, Satoh T, Tokunaga O, Sugawara K, Mizokami H, Kimoto M. The endothelial cell protein C receptor (EPCR) functions as a primary receptor for protein C activation on endothelial cells in arteries, veins, and capillaries. Biochem Biophys Res Commun. 1999; 259: 671–677.[CrossRef][Medline] [Order article via Infotrieve]
61. Dudek AZ, Pennell CA, Decker TD, Young TA, Key NS, Slungaard A. Platelet factor 4 binds to glycanated forms of thrombomodulin and to protein C. A potential mechanism for enhancing generation of activated protein C. J Biol Chem. 1997; 272: 31785–31792.
62. Slungaard A, Fernández JA, Griffin JH, Key NS, Long JR, Piegors DJ, Lentz SR. Platelet factor 4 enhances generation of activated protein C in vitro and in vivo. Blood. 2003; 102: 146–151.
63. Mosnier LO, Bouma BN, and Griffin JH Platelet factor 4 up-regulates protein C activation relative to TAFI activation (Abstract). J Thromb Haemost. 2003; 1, Supplement 1: OC376.
64. Rezaie AR, Cooper ST, Church FC, Esmon CT. Protein C inhibitor is a potent inhibitor of the thrombin-thrombomodulin complex. J Biol Chem. 1995; 270: 25336–25339.
65. Yang L, Manithody C, Walston TD, Cooper ST, Rezaie AR. Thrombomodulin enhances the reactivity of thrombin with protein C inhibitor by providing both a binding site for the serpin and allosterically modulating the activity of thrombin. J Biol Chem. 2003; 278: 37465–37470.
66. Elisen MG, Von dem Borne PAK, Bouma BN, Meijers JCM. Protein C inhibitor acts as a procoagulant by inhibiting the thrombomodulin-induced activation of protein C in human plasma. Blood. 1998; 91: 1542–1547.
67. Mosnier LO, Elisen MG, Bouma BN, Meijers JCM. Protein C inhibitor regulates the thrombin-thrombomodulin complex in the up- and down regulation of fibrinolysis. Thromb Haemost. 2001; 86: 1057–1064.[Medline] [Order article via Infotrieve]
68. Dahlbäck B, Villoutreix BO. Regulation of blood coagulation by the protein C anticoagulant pathway: novel insights into structure-function relationships and molecular recognition. Arterioscler Thromb Vasc Biol. 2005; 25: 1311–1320.
69. Hackeng TM, Sere KM, Tans G, Rosing J. Protein S stimulates inhibition of the tissue factor pathway by tissue factor pathway inhibitor. Proc Natl Acad Sci U S A. 2006; 103: 3106–3111.
70. Mosnier LO, Meijers JCM, Bouma BN. The role of protein S in the activation of thrombin activatable fibrinolysis inhibitor (TAFI) and regulation of fibrinolysis. Thromb Haemost. 2001; 86: 1040–1046.[Medline] [Order article via Infotrieve]
71. Sixma JJ, van den Berg A. The haemostatic plug in haemophilia A: a morphological study of haemostatic plug formation in bleeding time skin wounds of patients with severe haemophilia A. Br J Haematol. 1984; 58: 741–753.[Medline] [Order article via Infotrieve]
72. Walsh PN, Rizza CR, Matthews JM, Eipe J, Kernoff PB, Coles MD, Bloom AL, Kaufman BM, Beck P, Hanan CM, Biggs R. Epsilon-Aminocaproic acid therapy for dental extractions in haemophilia and Christmas disease: a double blind controlled trial. Br J Haematol. 1971; 20: 463–475.[Medline] [Order article via Infotrieve]
73. Wang W, Nagashima M, Schneider M, Morser J, Nesheim ME. Elements of the primary structure of thrombomodulin required for efficient thrombin-activable fibrinolysis inhibitor activation. J Biol Chem. 2000; 275: 22942–22947.
74. Lisman T, Mosnier LO, Lambert T, Mauser-Bunschoten E, Meijers JCM, Nieuwenhuis HK, de Groot PG. Inhibition of fibrinolysis by recombinant factor VIIa in plasma from patients with severe haemophilia A. Blood. 2002; 99: 175–179.
75. Lisman T, de Groot PG, Lambert T, Rojkjaer R, Persson E. Enhanced in vitro procoagulant and antifibrinolytic potential of superactive variants of recombinant factor VIIa in severe hemophilia A. J Thromb Haemost. 2003; 1: 2175–2178.[CrossRef][Medline] [Order article via Infotrieve]
76. Boffa MB, Bell R, Stevens WK, Nesheim ME. Roles of thermal instability and proteolytic cleavage in regulation of activated thrombin-activable fibrinolysis inhibitor. J Biol Chem. 2000; 275: 12868–12878.
77. Marx PF, Hackeng TM, Dawson PE, Griffin JH, Meijers JCM, Bouma BN. Inactivation of active thrombin-activable fibrinolysis inhibitor takes place by a process that involves conformational instability rather than proteolytic cleavage. J Biol Chem. 2000; 275: 12410–12415.
78. Marx PF, Dawson PE, Bouma BN, Meijers JCM. Plasmin-mediated activation and inactivation of thrombin-activatable fibrinolysis inhibitor. Biochemistry. 2002; 41: 6688–6696.[CrossRef][Medline] [Order article via Infotrieve]
79. Marx PF, Havik SR, Marquart JA, Bouma BN, Meijers JCM. Generation and characterization of a highly stable form of activated thrombin-activable fibrinolysis inhibitor. J Biol Chem. 2004; 279: 6620–6628.
80. Knecht W, Willemse J, Stenhamre H, Andersson M, Berntsson P, Furebring C, Harrysson A, Hager AC, Wissing BM, Hendriks D, Cronet P. Limited mutagenesis increases the stability of human carboxypeptidase U (TAFIa) and demonstrates the importance of CPU stability over proCPU concentration in down-regulating fibrinolysis. FEBS J. 2006; 273: 778–792.[CrossRef][Medline] [Order article via Infotrieve]
81. Ceresa E, Van de BK, Peeters M, Lijnen HR, Declerck PJ, Gils A. Generation of a stable TAFIa (activated thrombin activatable fibrinolysis inhibitor) variant. J Biol Chem. 2006; 281: 15878–15883.
82. van Tilburg NH, Rosendaal FR, Bertina RM. Thrombin activatable fibrinolysis inhibitor and the risk for deep vein thrombosis. Blood. 2000; 95: 2855–2859.
83. Koster T, Blann AD, Vandenbroucke JP, Rosendaal FR. Role of clotting factor VIII in effect of von Willebrand factor on occurence of deep-vein thrombosis. Lancet. 2000; 345: 152–155.
84. Vlieg AV, van der Linden IK, Bertina RM, Rosendaal FR. High levels of factor IX increase the risk of venous thrombosis. Blood. 2000; 95: 3678–3682.
85. Meijers JCM, Tekelenburg WL, Bouma BN, Bertina RM, Rosendaal FR. High levels of coagulation factor XI as a risk factor for venous thrombosis. N Engl J Med. 2000; 342: 696–701.
86. Eichinger S, Schonauer V, Weltermann A, Minar E, Bialonczyk C, Hirschl M, Schneider B, Quehenberger P, Kyrle PA. Thrombin-activatable fibrinolysis inhibitor and the risk for recurrent venous thromboembolism. Blood. 2004; 103: 3773–3776.
87. Juhan-Vague I, Morange PE. Very high TAFI antigen levels are associated with a lower risk of hard coronary events: the PRIME Study. J Thromb Haemost. 2003; 1: 2243–2244.[CrossRef][Medline] [Order article via Infotrieve]
88. Juhan-Vague I, Morange PE, Aubert H, Henry M, Aillaud MF, Alessi MC, Samnegard A, Hawe E, Yudkin J, Margaglione M, Di MG, Hamsten A, Humphries SE. Plasma thrombin-activatable fibrinolysis inhibitor antigen concentration and genotype in relation to myocardial infarction in the north and south of Europe. Arterioscler Thromb Vasc Biol. 2002; 22: 867–873.
89. Leurs J, Hendriks D. Carboxypeptidase U (TAFIa): a metallocarboxypeptidase with a distinct role in haemostasis and a possible risk factor for thrombotic disease. Thromb Haemost. 2005; 94: 471–487.[Medline] [Order article via Infotrieve]
90. Salomon O, Steinberg DM, Dardik R, Rosenberg N, Zivelin A, Tamarin I, Ravid B, Berliner S, Seligsohn U. Inherited factor XI deficiency confers no protection against acute myocardial infarction. J Thromb Haemost. 2003; 1: 658–661.[CrossRef][Medline] [Order article via Infotrieve]
91. Bouma BN, Meijers JCM. New insights into factors affecting clot stability: A role for thrombin activatable fibrinolysis inhibitor (TAFI; plasma procarboxypeptidase B, plasma procarboxypeptidase U, procarboxypeptidase R). Semin Hematol. 2004; 41: 13–19.[Medline] [Order article via Infotrieve]
92. Barbosa Pereira PJ, Segura-Martin S, Oliva B, Ferrer-Orta C, Aviles FX, Coll M, Gomis-Ruth FX, Vendrell J. Human procarboxypeptidase B: three-dimensional structure and implications for thrombin-activatable fibrinolysis inhibitor (TAFI). J Mol Biol. 2002; 321: 537–547.[CrossRef][Medline] [Order article via Infotrieve]
This article has been cited by other articles:
 |
|
 |
|
  M. E. Meltzer, C. J.M. Doggen, P. G. de Groot, J. C.M. Meijers, F. R. Rosendaal, and T. Lisman Low thrombin activatable fibrinolysis inhibitor activity levels are associated with an increased risk of a first myocardial infarction in men Haematologica, June 1, 2009; 94(6): 811 - 818. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Tanaka, N. S. Key, and J. H. Levy Blood Coagulation: Hemostasis and Thrombin Regulation Anesth. Analg., May 1, 2009; 108(5): 1433 - 1446. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. I. Tucker, U. M. Marzec, T. C. White, S. Hurst, S. Rugonyi, O. J. T. McCarty, D. Gailani, A. Gruber, and S. R. Hanson Prevention of vascular graft occlusion and thrombus-associated thrombin generation by inhibition of factor XI Blood, January 22, 2009; 113(4): 936 - 944. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Biscetti Glycosylation of Thrombin Activatable Fibrinolysis Inhibitor: Why Is it So Important? Circ. Res., February 15, 2008; 102(3): 278 - 279. [Full Text] [PDF]
|
|
|
|
|
|
M. B. Boffa, D. Maret, J. D. Hamill, N. Bastajian, P. Crainich, N. S. Jenny, Z. Tang, E. M. Macy, R. P. Tracy, R. F. Franco, et al. Effect of single nucleotide polymorphisms on expression of the gene encoding thrombin-activatable fibrinolysis inhibitor: a functional analysis Blood, January 1, 2008; 111(1): 183 - 189. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. O. Mosnier, X. V. Yang, and J. H. Griffin Activated Protein C Mutant with Minimal Anticoagulant Activity, Normal Cytoprotective Activity, and Preservation of Thrombin Activable Fibrinolysis Inhibitor-dependent Cytoprotective Functions J. Biol. Chem., November 9, 2007; 282(45): 33022 - 33033. [Abstract] [Full Text] [PDF]
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||