This Article Abstract Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Rotllan, N. Articles by Escolà-Gil, J. C. Search for Related Content PubMed Articles by Rotllan, N. Articles by Escolà-Gil, J. C.

(Arteriosclerosis, Thrombosis, and Vascular Biology. 2005;25:1896.)
© 2005 American Heart Association, Inc.

Atherosclerosis and Lipoproteins

Overexpression of Human Apolipoprotein A-II in Transgenic Mice Does Not Impair Macrophage-Specific Reverse Cholesterol Transport In Vivo

Noemí Rotllan; Vicent Ribas; Laura Calpe-Berdiel; Jesús M. Martín-Campos; Francisco Blanco-Vaca; Joan Carles Escolà-Gil

	Abstract

Top Abstract Web Site Feature

 
Background— Overexpression of human apolipoprotein (apo) A-II in transgenic mice induces high-density lipoprotein (HDL) deficiency, and increased atherosclerosis susceptibility only when fed an atherogenic diet. This may, in part, be caused by impairment in reverse cholesterol transport (RCT).

Methods and Results— [³H]cholesterol-labeled macrophages were injected intraperitoneally into mice maintained on a chow diet or an atherogenic diet. Plasma [³H]cholesterol did not differ from human apoA-II transgenic and control mice at 24 or 48 hours after the label injection. On the chow diet, human apoA-II transgenic mice presented increased [³H]cholesterol in liver (1.3-fold) and feces (6-fold) compared with control mice (P<0.05). The magnitude of macrophage-specific RCT did not differ between transgenic and control mice fed the atherogenic diet.

Conclusions— Human apoA-II maintains effective RCT from macrophages to feces in vivo despite an HDL deficiency. These findings suggest that the increased atherosclerotic lesions observed in apoA-II transgenic mice fed an atherogenic diet are not caused by impairment in macrophage-specific RCT.

Key Words: apolipoprotein A-II • atherosclerosis • high-density lipoprotein • macrophages • reverse cholesterol transport

	Web Site Feature

Top Abstract Web Site Feature

 
The full-length article can be found on the World Wide Web at http://www.atvbaha.org

This Article Abstract Full Text (PDF) Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Rotllan, N. Articles by Escolà-Gil, J. C. Search for Related Content PubMed Articles by Rotllan, N. Articles by Escolà-Gil, J. C.