Data Supplement for Arteriosclerosis, Thrombosis, and Vascular Biology: Volume 20, Issue 2
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Data Files
U990532FigI.jpg
- Figure I. A vector expressing murine FasL (AdFasL) causes apoptosis of rabbit SMCs. A and B, Phase-contrast micrographs of cultured SMCs 18 hours after transduction with AdNull (vector without a transgene) and AdFasL. Original magnification X200. C, Agarose gel electrophoresis of DNA extracted from SMC that were mock transduced or transduced with the two vectors. DNA size markers (HaeIII-digested (X-174 RF) are at left.
U990532FigII.jpg
- Figure II. Western blot detection of FasL expression. FasL expression was analyzed in lysates from untransduced 293 cells (293) endothelial cells (EC) transduced with AdFasL in vivo (EC-FasL), 293 cells transduced with FasL (293-FasL), EC transduced in vivo with AdRSVnLacZ (EC-lacZ), and arteries transduced in vivo with AdFasL or AdRSVnLacZ and denuded of endothelium ex vivo before analysis (Denuded-FasL and data not shown). Lysates were made 24 hours after gene transfer. The intensity of a nonspecific protein band present in all arterial lysates (not shown) confirmed equal loading. This experiment was repeated once with similar results.
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