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Data Supplement for Arteriosclerosis, Thrombosis, and Vascular Biology: Volume 20, Issue 12 -- Page 2533
This item has the following additional materials available:
Data Files
- Figure I. Concentration-dependent stimulation of MMP-1 secretion by U937 cells treated with LDL-ICs. U937 cells were incubated at 37°C for 24 h with increasing concentrations of LDL-ICs as indicated. After the incubation, the conditioned medium was subjected to ELISA to determine the amount of secreted MMP-1, and the cells were lysed for DNA assay as described in Methods.
- Figure II. Time course of LDL-IC–stimulated MMP-1 secretion by U937 cells. U937 cells were incubated with medium alone (control) or medium containing 150 μg/mL LDL-ICs for different times as indicated. After the incubation, the conditioned medium was subjected to ELISA to quantitate secreted MMP-1 as described in Methods.
- Figure III. Inhibition of LDL-IC–stimulated MMP-1 secretion by Ro-31-8220. U937 cells were incubated for 24 h with 150 μg/mL LDL-ICs in the absence or presence of increasing concentrations of Ro-31-8220 or DMSO as indicated. After the incubation, the conditioned medium was subjected to ELISA to quantitate secreted MMP-1. The amount of secreted MMP-1 by LDL-IC–stimulated cells in the absence of Ro-31-8220 was designated as 100%. 0.1% DMSO, a vehicle for 80 nmol/L Ro-31-8220, was used as control. The data presented represents the mean±SEM of 3 different experiments run in duplicate.
- Figure IV. Inhibition of MAPK phosphorylation in U937 cells by Ro-31-8220. U937 cells were incubated with 150 μg/mL LDL-ICs for the times indicated in the absence or presence of 80 nmol/L of Ro-31-8220. The cells were then lysed and cellular MAPK phosphorylation was analyzed by Western blot as described in Figure 4 legend.
- Figure V. Stimulation of MMP-1 secretion by IgG-containing immune complexes. U937 cells were incubated for 24 h with culture medium alone (control) or the culture medium containing 150 μg/mL IgG-containing immune complexes (IgG-ICs). After the incubation, conditioned medium was subjected to ELISA to quantitate secreted MMP-1 as described in Methods. The data presented represents the mean±SEM of 3 experiments run in duplicate.
- Figure VI. Stimulation of MMP-1 secretion from human monocyte–derived macrophages by LDL-ICs. Human monocyte–derived macrophages were incubated for 24 h with medium alone (control) or medium containing 150 μg/mL LDL-ICs, 100 μg/mL native LDL (nLDL), or 100 nmol/L PMA. After the incubation, conditioned medium was subjected to ELISA to determine amount of secretion MMP-1 as described in Methods. The data presented represents the mean±SEM of 3 experiments run in duplicate.
Prepared by: the Data Supplement Manager
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