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Data Supplement for Arteriosclerosis, Thrombosis, and Vascular Biology: Volume 20, Issue 11 -- Page 2366
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Data Files
Figure I. Increasing flow from 0.2 to 40 mL/min in the absence of pulsatility (2%) does not affect ERK1/2 activity in aortic segments kept at 80 mm Hg for 24 hours. Also, ERK1/2 activation by 10% pulsatility is not further enhanced by 20% pulsatility. Upper panel: representative autoradiogram of in-gel kinase assay of ERK1 and ERK2 activity. Lower panel: quantification of in-gel ERK1/2 assays. Results are representative of 4 to 6 separate experiments. *P<0.05 vs no pulsatility (0.5%).
Figure II. Activation of p38 MAP kinase by pulsatility (10%) in aortic segments kept at 80 mm Hg for 24 hours is prevented by polyethylene glycol-superoxide dismutase (SOD, 40 IU/mL). Representative Western blot using anti-phospho-p38 antibodies. Results are representative of 3 separate experiments.
Prepared by: the Data Supplement Manager
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