on May 1, 2008
Published online before print May 1, 2008, doi: 10.1161/ATVBAHA.107.160382
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Submitted on November 27, 2007
Accepted on April 21, 2008
Androgen Increases AT1a Receptor Expression in Abdominal Aortas to Promote Angiotensin II–Induced AAAs in Apolipoprotein E–Deficient Mice
Tracy Henriques ;
From the Graduate Center for Toxicology (T.H., X.Z.), Cardiovascular Research Center (A.D.), and the Graduate Center for Nutritional Sciences (F.B.Y., L.A.C.), University of Kentucky, Lexington.
* To whom correspondence should be addressed. E-mail: lcassis{at}uky.edu.
Objective—Castration of male apolipoprotein E–deficient (apoE-/-) mice reduces angiotensin II (Ang II)–induced abdominal aorta aneurysms (AAAs) to that of female mice. The purpose of this study was to determine whether this reduction is attributable to androgen-mediated regulation of aortic Ang II type 1A receptors (AT1aR).
Methods and Results—AT1aR mRNA abundance in the AAA-prone region of abdominal aortas was 8-fold greater compared to thoracic aortas of male but not female mice. AT1aR mRNA abundance decreased after castration in abdominal but not thoracic aortas of male mice. Dihydrotestosterone (DHT, 0.16 mg/d) administration to castrated male mice restored AT1aR mRNA abundance in abdominal aortas but had no effect in thoracic aortas. DHT also increased AT1aR mRNA abundance in abdominal aortas from female mice. Castrated male or female apoE-/- mice were administered DHT during infusion of saline or Ang II (1000 ng/kg/min for 28 days). DHT administration did not alter serum cholesterol concentrations, lipoprotein distributions, or atherosclerotic lesion areas in either male or female mice. However, administration of DHT increased AAA incidence in male (27% placebo versus 75% DHT) and female mice (28% placebo versus 64% DHT).
Conclusions—Androgen promotes AT1aR mRNA abundance in abdominal aortas associated with increased Ang II–induced AAAs.
Key words: angiotensin • aneurysms • androgen • atherosclerosis • sex hormones