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Arteriosclerosis, Thrombosis, and Vascular Biology. 1989;9:242-246

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Arteriosclerosis, Vol 9, 242-246, Copyright © 1989 by American Heart Association

ARTICLES

Bsp 12861 restriction fragment length polymorphism detects Ag(c/g) locus of human apolipoprotein B in all 17 persons studied

YH Ma, XB Wang, R Butler and VN Schumaker
Department of Chemistry, University of California, Los Angeles.

Southern blot analysis of the genomic DNA from 17 individuals showed perfect correspondence between a Bsp 12861 restriction fragment length polymorphism (RFLP) and the Ag(c/g) locus on human apolipoprotein (apo) B. The RFLP polymorphism is caused by a C--------T transition at nucleotide 421 on the cDNA, resulting in a threonine-to-isoleucine conversion. Thus, three of the five Ag sites have now been tentatively located on the 4536 residue apo B peptide at amino acyl residues 71: Ag(c/g), 591 (Ag(a1/d), and 4154 Ag(t/z).


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