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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2009;29:2090.)
© 2009 American Heart Association, Inc.

Integrative Physiology/Experimental Medicine

Mapping 3-Dimensional Neovessel Organization Steps Using Micro-Computed Tomography in a Murine Model of Hindlimb Ischemia–Brief Report

Pierre Oses; Marie-Ange Renault; Rémi Chauvel; Lionel Leroux; Cécile Allières; Benjamin Séguy; Jean-Marie Daniel Lamazière; Pascale Dufourcq; Thierry Couffinhal; Cécile Duplàa

From Inserm U828, Pessac, Université Victor Ségalen, Bordeaux, France (P.O., M.-A.R., R.C., L.L., C.A., B.S., J.-M.D.L., P.D., T.C., C.D.); CEPTA, Pôle Cardiothoracique, CHU Haut Lévêque, Pessac, France (P.O., B.S., T.C.); Laboratoire de Biochimie, UFR Sciences Pharmaceutiques, Université Victor Ségalen Bordeaux, France (P.D.).

Correspondence to Thierry Couffinhal, MD, PhD, Inserm U 828, Avenue du Haut-Lévêque, 33600 Pessac, France. E-mail thierry.couffinhal{at}inserm.fr

Objectives— Studying the mechanisms of neovascularization and evaluating the effects of proangiogenic strategies require accurate analysis of the neovascular network. We sought to evaluate the contribution of the microcomputed tomography (mCT) providing high-resolution 3-dimensional (3D) structural data, to a better comprehension of the well-studied mouse hindlimb postischemic neovascularization.

Methods and Results— We showed a predominant arteriogenesis process in the thigh and a predominant angiogenesis-related process in the tibiofibular region, in response to ischemia during the first 15 days. After 15 days, mCT quantitative analysis reveals a remodeling of arterial neovessels and a regression depending on the restoration of the blood flow. We provided also new mCT data on the rapid and potent angiogenic effects of mesenchymal stem cell therapy on vessel formation and organization. We discussed the contribution of this technique compared with or in addition to data generated by the more conventional approaches.

Conclusion— This study demonstrated that optimized mCT is a robust method for providing new insights into the 3D understanding of postischemic vessel formation.

We evaluated using microcomputed tomography (mCT) method mouse hindlimb postischemic neovascularization. We uncovered a predominant arteriogenesis and angiogenesis-related processes respectively in the thigh an tibiofibular regions. We provided new mCT data on the rapid and potent angiogenic effects of mesenchymal stem cell therapy on vessel formation and organization.

Key Words: computerized tomography • peripheral vascular disease • angiogenesis • mouse model of human disease