Published online before print September 4, 2008, doi: 10.1161/ATVBAHA.108.167965
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© 2008 American Heart Association, Inc.
Integrative Physiology/Experimental Medicine |
Estrogen-Stimulated Endothelial Repair Requires Osteopontin
From INSERM, U828 (L.L.S.L., S.J., F.R., D.D., C.A., C.D., P.D., C.D., A.-P.G.) and University of Bordeaux Victor Ségalen, IFR4 (L.L.S.L., S.J., F.R., D.D., C.A., C.D., P.D., C.D., A.-P.G.), Bordeaux, France; INSERM, U858 (C.F., A.B., B.G.-S., F.L., J.-F.A.) and University of Toulouse Paul Sabatier, CHU Toulouse-Rangueil, I2MR (C.F., A.B., B.G.-S., F.L., J.-F.A.), Toulouse, France; Forsyth Dental Institute (S.R.R.), Harvard University, Boston, Mass; Medizinische Klinik und Poliklinik II (N.W., G.N.), University of Bonn, Germany; and the Theodor Kocher Institute (U.D.), University of Bern, Switzerland.
Correspondence to AP Gadeau, INSERM U828, Avenue du Haut Lévèque, 33600 Pessac, France. E-mail alain.gadeau{at}inserm.fr
Objective— Estradiol (E2) is known to accelerate reendothelialization and thus prevent intimal thickening and in-stent restenosis after angioplasty. Transplantation experiments with ER
–/– mice have previously shown that E2 acts through local and bone marrow cell compartments to enhance endothelial healing. However, the downstream mechanisms induced by E2 to mediate endothelial repair are still poorly understood.
Methods and Results— We show here that after endovascular carotid artery injury, E2-enhanced endothelial repair is lost in osteopontin-deficient mice (OPN–/–). Transplantation of OPN–/– bone marrow into wild-type lethally irradiated mice, and vice versa, suggested that osteopontin plays a crucial role in both the local and the bone marrow actions of E2. In the vascular compartment, using transgenic mice expressing doxycyclin regulatable-osteopontin, we show that endothelial cell specific osteopontin overexpression mimics E2-enhanced endothelial cell migration and proliferation in the regenerating endothelium. In the bone marrow cell compartment, we demonstrate that E2 enhances bone marrow–derived mononuclear cell adhesion to regenerating endothelium in vivo, and that this effect is dependent on osteopontin.
Conclusions— We demonstrate here that E2 acceleration of the endothelial repair requires osteopontin, both for bone marrow–derived cell recruitment and for endothelial cell migration and proliferation.
Estradiol (E2) is known to accelerate endothelial repair and thus prevent intimal thickening and in-stent restenosis after angioplasty. However, the mechanisms are poorly understood.
Key Words: endothelium • endothelial cells • hormone • bone marrow cells • wound healing • osteopontin
Related Article:
Arterioscler Thromb Vasc Biol 2008 28: 2099-2100.
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  D. G.M. Molin, N. M.S van den Akker, and M. J. Post Affirmative Action of Osteopontin on Endothelial Progenitors Arterioscler Thromb Vasc Biol, December 1, 2008; 28(12): 2099 - 2100. [Full Text] [PDF]
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