This Article Full Text Full Text (PDF) Data Supplement All Versions of this Article: 26/10/2337    most recent 01.ATV.0000238357.60338.90v1 Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ditiatkovski, M. Articles by Bobik, A. Search for Related Content PubMed PubMed Citation Articles by Ditiatkovski, M. Articles by Bobik, A.

(Arteriosclerosis, Thrombosis, and Vascular Biology. 2006;26:2337.)
© 2006 American Heart Association, Inc.

Atherosclerosis and Lipoproteins

GM-CSF Deficiency Reduces Macrophage PPAR- Expression and Aggravates Atherosclerosis in ApoE-Deficient Mice

Michael Ditiatkovski; Ban-Hock Toh; Alex Bobik

From the Cell Biology Laboratory (M.D., A.B.), Baker Heart Research Institute and Autoimmunity Laboratory (M.D., B.-H.T.), Department of Immunology, Monash University, AMREP, Alfred Campus, Melbourne, Victoria, Australia. Present address for B.-H.T.: Centre for Inflammatory Diseases, Monash Institute of Medical Research, Monash University, Clayton, Victoria 3168, Australia.

Correspondence to Michael Ditiatkovski, Cell Biology Laboratory, Baker Heart Research Institute, PO Box 6492, St Kilda Road Central, Melbourne, Victoria, 8008, Australia. E-mail Michael.Ditiatkovski{at}baker.edu.au

Objective— Granulocyte-macrophage colony-stimulating factor (GM-CSF) is expressed in atherosclerotic lesions but its significance for lesion development is unknown. Consequently, we investigated the significance of GM-CSF expression for development of atherosclerotic lesions in apolipoprotein E-deficient (apoE^–/–) mice.

Methods and Results— We generated apoE^–/– mice deficient in GM-CSF (apoE^–/–.GM-CSF^–/– mice), fed them a high-fat diet, and compared lesion development with apoE^–/– mice. We measured lesion size, macrophage, smooth muscle cell, and collagen accumulation at the aortic sinus, and expression of genes that regulate cholesterol transport and inflammation. No differences in serum cholesterol were found between the 2 groups. Lesion size in hyperlipidemic apoE^–/–.GM-CSF^–/– increased by 30% (P<0.05), macrophage accumulation doubled, and collagen content reduced by 15% (P<0.05); smooth muscle cell accumulation and vascularity were unaffected. Analysis of PPAR-, ABCA1, and CD36 in lesions showed reduced expression (50%, 65%, and 55%, respectively), whereas SR-A doubled. In peritoneal macrophages, PPAR- and ABCA1 expression was also reduced by 50% and 70%, respectively, as was cholesterol efflux, by 50%. In lesions, pro-inflammatory MCP-1 and tumor necrosis factor (TNF)- expression increased 2- and 3.5-fold, respectively, vascular cell adhesion molecule (VCAM)-1 expression enhanced and interleukin (IL)-1 receptor antagonist reduced by 50%.

Conclusions— GM-CSF deficiency increases atherosclerosis under hypercholesterolemic conditions, indicating antiatherogenic role for GM-CSF. We suggest this protective role is mediated by PPAR- and ABCA1, molecules that affect cholesterol homeostasis and inflammation.

We studied effect of GM-CSF deletion on atherosclerosis in apoE^–/– mice. We observed increases in lesion size, macrophage accumulation, MCP-1, TNF-, and VCAM-1; decreases in collagen content, PPAR-, ABCA1, and cholesterol efflux from macrophages. GM-CSF promotes smaller stable atherosclerotic lesions by mechanisms dependant on PPAR- and ABCA1.

Key Words: apoE^–/– mice • atherosclerotic lesions • GM-CSF deficiency • hyperlipidemia • inflammatory cytokines • PPAR-

This article has been cited by other articles:

				J. A. Wingrove, S. E. Daniels, A. J. Sehnert, W. Tingley, M. R. Elashoff, S. Rosenberg, L. Buellesfeld, E. Grube, L. K. Newby, G. S. Ginsburg, et al. Correlation of Peripheral-Blood Gene Expression With the Extent of Coronary Artery Stenosis Circ Cardiovasc Genet, October 1, 2008; 1(1): 31 - 38. [Abstract] [Full Text] [PDF]

				R. Kleemann, S. Zadelaar, and T. Kooistra Cytokines and atherosclerosis: a comprehensive review of studies in mice Cardiovasc Res, August 1, 2008; 79(3): 360 - 376. [Abstract] [Full Text] [PDF]

				S. W. Waldo, Y. Li, C. Buono, B. Zhao, E. M. Billings, J. Chang, and H. S. Kruth Heterogeneity of Human Macrophages in Culture and in Atherosclerotic Plaques Am. J. Pathol., April 1, 2008; 172(4): 1112 - 1126. [Abstract] [Full Text] [PDF]

				M. J. Thomassen, B. P. Barna, A. G. Malur, T. L. Bonfield, C. F. Farver, A. Malur, H. Dalrymple, M. S. Kavuru, and M. Febbraio ABCG1 is deficient in alveolar macrophages of GM-CSF knockout mice and patients with pulmonary alveolar proteinosis J. Lipid Res., December 1, 2007; 48(12): 2762 - 2768. [Abstract] [Full Text] [PDF]

				A. Haghighat, D. Weiss, M. K. Whalin, D. P. Cowan, and W. R. Taylor Granulocyte Colony-Stimulating Factor and Granulocyte Macrophage Colony-Stimulating Factor Exacerbate Atherosclerosis in Apolipoprotein E-Deficient Mice Circulation, April 17, 2007; 115(15): 2049 - 2054. [Abstract] [Full Text] [PDF]