This Article Full Text Full Text (PDF) All Versions of this Article: 25/8/1659    most recent 01.ATV.0000172660.24580.b4v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Caligiuri, G. Articles by Lafont, A. Search for Related Content PubMed PubMed Citation Articles by Caligiuri, G. Articles by Lafont, A. Related Collections Arterial thrombosis Platelet function inhibitors Animal models of human disease Pathophysiology

(Arteriosclerosis, Thrombosis, and Vascular Biology. 2005;25:1659.)
© 2005 American Heart Association, Inc.

Atherosclerosis and Lipoproteins

Reduced Immunoregulatory CD31⁺ T Cells in the Blood of Atherosclerotic Mice With Plaque Thrombosis

Giuseppina Caligiuri; Emilie Groyer; Jamila Khallou-Laschet; Ayman Al Haj Zen; Julie Sainz; Dominique Urbain; Anh-Thu Gaston; Mathilde Lemitre; Antonino Nicoletti; Antoine Lafont

From EMI0016 (G.C., A.A.H.Z., J.S., D.U., M.L., A.L.), Faculté de Médecine Paris, France; U681 (E.G., J.K.-L., A.-T.G., A.N.), Institut des Cordeliers, INSERM, Paris, France.

Correspondence to Giuseppina Caligiuri, MD, PhD, INSERM U681, Institut de Cordeliers; 15, rue de l’Ecole de Médecine, 75006 Paris, France. E-mail caligiuri{at}necker.fr or giuseppina.Caligiuri@u430.bhdc.jussieu.fr

Objective— Lymphocyte activation is thought to play a major role in the pathogenesis of atherosclerotic complications such as plaque thrombosis. Circulating CD31⁺ T cells have been shown to regulate human T cell activation. Aim of this study was to evaluate whether the proportion of circulating immunoregulatory CD31⁺ T cells is correlated to the occurrence of plaque thrombosis in aged apolipoprotein (apo) E knockout (KO) mice.

Methods and Results— CD31⁺ T cell depletion of spleen T cells enhanced proliferation (P<0.05) and interferon- production (P<0.01) while reducing interleukin (IL)-4 (P<0.001) and IL-10 (P=0.001) secretion in response to minimally modified low-density lipoprotein. CD31⁺ T cells were counted in 65 apoE KO mice (46-week-old) by flow cytometry. Organizing thrombi could be documented in 28 of 195 (14%) lesions and in at least one of the aorta root lesions in 23 of 65 mice (35%). CD31⁺ T cell count was significantly reduced in mice showing plaque thrombosis (72.3±1.5% versus 84.1±1.2%; P<0.0001), but such reduction did not follow induced plaque rupture or experimentally controlled thrombosis.

Conclusions— Reduced CD31⁺ T cells in circulating blood is a hallmark of atherosclerotic plaque thrombosis. Our data suggest that CD31⁺ T cells may play an important regulatory role in the development of plaque thrombosis.

Lymphocyte activation plays a major role in the pathogenesis of plaque thrombosis. CD31⁺ T cells regulate T cells activation. We found that circulating CD31⁺ T cells were reduced in apolipoprotein E knockout mice with plaque thrombosis. Our data suggest that CD31⁺ T cells may play a regulatory role in plaque thrombosis.

Key Words: atherosclerosis • lymphocytes • thrombosis

This article has been cited by other articles:

				J. Hur, H.-M. Yang, C.-H. Yoon, C.-S. Lee, K.-W. Park, J.-H. Kim, T.-Y. Kim, J.-Y. Kim, H.-J. Kang, I.-H. Chae, et al. Identification of a Novel Role of T Cells in Postnatal Vasculogenesis: Characterization of Endothelial Progenitor Cell Colonies Circulation, October 9, 2007; 116(15): 1671 - 1682. [Abstract] [Full Text] [PDF]

				E. Groyer, A. Nicoletti, H. Ait-Oufella, J. Khallou-Laschet, A. Varthaman, A.-T. Gaston, O. Thaunat, S. V. Kaveri, R. Blatny, H. Stockinger, et al. Atheroprotective Effect of CD31 Receptor Globulin Through Enrichment of Circulating Regulatory T-Cells J. Am. Coll. Cardiol., July 24, 2007; 50(4): 344 - 350. [Abstract] [Full Text] [PDF]

				A. Mor, G. Luboshits, D. Planer, G. Keren, and J. George Altered status of CD4+CD25+ regulatory T cells in patients with acute coronary syndromes Eur. Heart J., November 1, 2006; 27(21): 2530 - 2537. [Abstract] [Full Text] [PDF]

				G. Caligiuri and A. Nicoletti Lymphocyte responses in acute coronary syndromes: lack of regulation spawns deviant behaviour Eur. Heart J., November 1, 2006; 27(21): 2485 - 2486. [Full Text] [PDF]

				A.-K. L. Robertson and G. K Hansson T Cells in Atherogenesis: For Better or For Worse? Arterioscler. Thromb. Vasc. Biol., November 1, 2006; 26(11): 2421 - 2432. [Abstract] [Full Text] [PDF]

				G. Caligiuri, P. Rossignol, P. Julia, E. Groyer, D. Mouradian, D. Urbain, N. Misra, V. Ollivier, M. Sapoval, P. Boutouyrie, et al. Reduced Immunoregulatory CD31+ T Cells in Patients With Atherosclerotic Abdominal Aortic Aneurysm Arterioscler. Thromb. Vasc. Biol., March 1, 2006; 26(3): 618 - 623. [Abstract] [Full Text] [PDF]