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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2005;25:1280.)
© 2005 American Heart Association, Inc.

Thrombosis

Analysis of Gas6 in Human Platelets and Plasma

Istvan Balogh; Sassan Hafizi; Jonas Stenhoff; Karin Hansson; Björn Dahlbäck

From the Department of Laboratory Medicine, Division of Clinical Chemistry, Lund University, Wallenberg Laboratory, University Hospital Malmö, Sweden. Present address for I.B. is the Department of Clinical Biochemistry and Molecular Pathology, Medical and Health Science Center, University of Debrecen, Hungary.

Correspondence to Björn Dahlbäck MD, PhD, Professor of Blood Coagulation Research, University of Lund, Department of Laboratory Medicine, Division of Clinical Chemistry, Wallenberg laboratory, University Hospital, Malmö, S-20502 Malmö, Sweden. E-mail bjorn.dahlback{at}klkemi.mas.lu.se

Objective— Gas6 is a member of the vitamin K-dependent protein family. Gas6-deficient mice were found to be resistant to thrombosis because of defective platelet function. Mouse Gas6 was demonstrated to be present in platelets and found to be involved in platelet aggregation. The aim of this study was to investigate the presence of Gas6 in human platelets and plasma and determine its role in platelet function.

Methods and Results— The presence of Gas6 in human platelets and plasma was analyzed using sensitive immunologic methods. Mass spectrometry and ELISA were used to identify and quantify Gas6 in plasma. Gas6 was demonstrated to be present in human plasma, at a concentration determined to be 13 to 23 ng/mL (0.16 to 0.28 nM). Furthermore, plasma Gas6 levels were found to be lower in patients administered with warfarin. However, Gas6 was undetectable in human platelets.

Conclusions— This is the first report to identify and quantify Gas6 in human plasma. However, Gas6 protein was not detected in human platelets, suggesting that any potential platelet-specific function could be because of Gas6 from the circulation. These findings open up new directions regarding the role of Gas6 in normal and pathophysiological situations such as inflammation, autoimmune disease, thrombosis and arteriosclerosis.

The aim of this study was to analyze the presence of Gas6 in human plasma and platelets. Using sensitive immune-based methods, we detected Gas6 in the human circulation in the sub-nanomolar range, the concentration of which was slightly decreased in warfarin-treated patients. However, we could not detect Gas6 in human platelets.

Key Words: Gas6 • protein S • vitamin K • Gla • warfarin • platelets

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