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(Arteriosclerosis, Thrombosis, and Vascular Biology. 2005;25:359.)
© 2005 American Heart Association, Inc.

Vascular Biology

In Vitro Angiogenic Effects of Pancreatic Bile Salt-Dependent Lipase

Ouafa Rebaï; Josette Le Petit-Thevenin; Nadine Bruneau; Dominique Lombardo; Alain Vérine

From INSERM U-559 and IPHM, Marseille-cedex, France.

Correspondence to Dr D. Lombardo, INSERM, U-559, Faculté de Médecine-Timone, 27 bld Jean MOULIN, 13385, Marseille-cedex 05 France. E-mail dominique.lombardo{at}medecine.univ-mrs.fr

Objective— Bile salt-dependent lipase (BSDL), a lipolytic enzyme secreted in the duodenum by pancreatic acinar cells, has been detected in the serum of all patients and in atheromatous plaque, suggesting its potential implication in vascular pathophysiology.

Methods and Results— In vitro pancreatic BSDL evokes human umbilical vein endothelial cell (HUVEC) proliferation and chemotactic migration. BSDL at mitogen concentration is capable to heal wounded HUVEC monolayer and to promote capillary network formation. HUVEC proliferation depends on the displacement of basic fibroblast growth factor and vascular endothelial growth factor from the extracellular matrix and the activation of extracellular signal-regulated kinases (ERK1/2), p38 mitogen-activated protein kinase, and focal adhesion kinase signaling pathways.

Conclusion— For the first time to our knowledge, it is suggested that circulating BSDL could be involved in pathophysiological angiogenesis.

We delineate the in vitro effects of pancreatic BSDL on endothelial cells, and we show that BSDL promotes proliferation, migration, capillary network formation, and wound-healing of HUVECs via the displacement of bFGF and VEGF from the ECM, suggesting that BSDL could be involved in angiogenesis.

Key Words: angiogenesis • human umbilical vein endothelial cells • bile salt-dependent lipase • growth factors

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